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The map label for this gene is dnaT [H]

Identifier: 157163810

GI number: 157163810

Start: 4604292

End: 4604831

Strand: Reverse

Name: dnaT [H]

Synonym: EcHS_A4594

Alternate gene names: 157163810

Gene position: 4604831-4604292 (Counterclockwise)

Preceding gene: 229220671

Following gene: 157163809

Centisome position: 99.17

GC content: 56.3

Gene sequence:

>540_bases
ATGTCTTCCAGAGTTTTGACCCCGGACGTCGTTGGTATTGACGCCCTGGTACACGATCACCAAACCGTTCTGGCAAAAGC
TGAAGGCGGTGTGGTTGCCGTATTTGCTAACAATGCCCCGGCGTTTTATGCCGTCACGCCTGCACGCCTGGCTGAACTGC
TGGCGCTGGAAGAAAAGCTGGCGCGTCCGGGAAGCGATGTCGCTCTGGACGATCAACTCTATCAGGAACCGCAAGCTGCT
CCCGTTGCTGTACCCATGGGGAAATTCGCCATGTATCCGGACTGGCAACCCGATGCCGACTTTATCCGCCTGGCGGCGCT
ATGGGGCGTGGCACTAAGAGAGCCGGTGACCACCGAAGAACTGACCTCATTCATTGCCTACTGGCAGGCGGAAGGTAAAG
TCTTTCACCATGTACAGTGGCAACAAAAACTGGCGCGCAGCCTGCAAATCGGTCGTGCCAGCAACGGCGGACTGCCGAAA
CGAGATGTGAATACGGTCAGTGAACCTGACAGCCAAATTCCACCAGGATTCAGAGGGTAA

Upstream 100 bases:

>100_bases
TCGCTTAAAGCACGCGGATGAGAGACAGCCACCTCTCCTCCGTGTGTTACTATAAAAGTTATCTCCCTTCTCGTTCATCG
TTCCATATTTGAGAAACAGT

Downstream 100 bases:

>100_bases
CGATGAAAAACGTTGGCGACCTGATGCAACGCCTGCAAAAAATGATGCCTGCCCATATCAAACCTGCATTCAAAACGGGT
GAAGAACTACTGGCCTGGCA

Product: primosomal protein DnaI

Products: NA

Alternate protein names: Primosomal protein I [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAA
PVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
RDVNTVSEPDSQIPPGFRG

Sequences:

>Translated_179_residues
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAA
PVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
RDVNTVSEPDSQIPPGFRG
>Mature_178_residues
SSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAP
VAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKR
DVNTVSEPDSQIPPGFRG

Specific function: This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaT family [H]

Homologues:

Organism=Escherichia coli, GI1790824, Length=179, Percent_Identity=99.4413407821229, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020917 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 19485; Mature: 19354

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKL
CCCCCCCCCHHHHHHHHHCCHHEEEECCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHH
ARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEE
CCCCCCCEECHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
LTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKL
CCCCCCCCHHHHHHHHHCCHHEEEECCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHH
ARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEE
CCCCCCCEECHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
LTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA