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The map label for this gene is alr [H]

Identifier: 157163522

GI number: 157163522

Start: 4297526

End: 4298605

Strand: Direct

Name: alr [H]

Synonym: EcHS_A4293

Alternate gene names: 157163522

Gene position: 4297526-4298605 (Clockwise)

Preceding gene: 157163521

Following gene: 157163523

Centisome position: 92.55

GC content: 55.65

Gene sequence:

>1080_bases
ATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCACCTGCCAG
TAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTTCTTGAGACCGCGCGAACGCTCCCCGATGCTGACG
CCTTTGGCGTAGCCCGTCTCGAAGAAGCTCTGCGACTGCGTGCGGGGGGAATCACCAAACCTGTACTGTTACTCGAAGGC
TTTTTTGATGCCAGAGATCTGCCGACGATTTCTGCGCAACATTTTCATACCGCCGTGCATAACGAAGAACAGCTGGCTGC
GCTGGAAGAGGCTAGCCTGGACGAGCCGGTTACCGTCTGGATGAAACTCGATACCGGTATGCACCGTCTGGGCGTAAGGC
CGGAACAGGCTGAGGCGTTTTATCATCGCCTGACCCAGTGCAAAAACGTTCGTCAGCCGGTGAATATCGTCAGCCATTTT
GCGCGCGCGGATGAACCAAAATGTGGCGCAACCGAGAAACAACTCGCTATCTTTAATACCTTTTGCGAAGGCAAACCTGG
TCAACGTTCCATTGCCGCATCGGGTGGCATTCTGCTGTGGCCACAGTCGCATTTTGACTGGGTGCGCCCGGGCATCATTC
TTTATGGCGTCTCGCCGCTGGAAGATCGCTCCACCGGTGCCGATTTTGGCTGTCAGCCAGTGATGTCACTAACCTCCAGC
CTGATTGCCGTGCGTGAGCATAAAGCCGGAGAACCTGTTGGTTATGGTGGAACCTGGGTAAGCGAACGTGATACCCGTCT
TGGCGTAGTCGCGATGGGCTATGGCGATGGTTATCCGCGCGCCGCGCCGTCCGGTACGCCAGTGCTGGTGAACGGTCGCG
AAGTACCGATTGTCGGGCGCGTGGCGATGGATATGATCTGCGTAGACTTAGGTCCACAGGCGCAGGACAAAGCCGGGGAT
CCGGTCATTTTATGGGGCGAAGGTTTGCCCGTAGAACGTATCGCTGAAATGACGAAAGTAAGCGCTTACGAACTTATTAC
GCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAA

Upstream 100 bases:

>100_bases
TGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAA
ACACTTATCAAGGAACACAA

Downstream 100 bases:

>100_bases
TCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCCTAAAAATTCAATA
AGTTGATGTTCTTTCATGCT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD

Sequences:

>Translated_359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
>Mature_359_residues
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEG
FFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
LIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGD
PVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=359, Percent_Identity=100, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1787439, Length=346, Percent_Identity=42.1965317919075, Blast_Score=277, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 39153; Mature: 39153

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHH
EEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVW
HHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
MKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT
EEECCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
HHCCCCCCCEEECCCCEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCEEHH
VAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARL
CCCEEEEHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHH
EEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVW
HHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEE
MKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT
EEECCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSS
HHCCCCCCCEEECCCCEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCCEEHH
VAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVAMKYVD
HHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]