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The map label for this gene is dusA [H]

Identifier: 157163519

GI number: 157163519

Start: 4293407

End: 4294450

Strand: Direct

Name: dusA [H]

Synonym: EcHS_A4290

Alternate gene names: 157163519

Gene position: 4293407-4294450 (Clockwise)

Preceding gene: 157163518

Following gene: 157163521

Centisome position: 92.46

GC content: 53.45

Gene sequence:

>1044_bases
TTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAG
TGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATA
CGTTGCTTTATACCGAAATGGTGACCACAGGGGCGATTATTCACGGTAAAGGTGATTATCTGGCGTACAGTGAAGAAGAA
CATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATA
TGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATG
CGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATC
GACGACCAGGACAGCTATGAATTTCTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCAT
CCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGT
ATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACAC
CTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGAT
CTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCC
AGGGGACGTATCTCGGCCATATTACCCGGCATATGCTGGGTTTGTTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGT
TATTTAAGTGAAAATGCCCATAAAGCGGGTGCTGACATTAACGTGCTGGAACACGCGCTCAAACTGGTGGCGGATAAGCG
TTAA

Upstream 100 bases:

>100_bases
GCTTCCTGTTGTTATGGTATAGTACCCCGCTATTGAGCCTCCTGAACAGTGAAGCTGAATAACATAACCCCATGATATAT
TGATAAAATAATCTCTACAT

Downstream 100 bases:

>100_bases
CTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATT
TTTGGCATGATTCTTGTAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR

Sequences:

>Translated_347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR
>Mature_347_residues
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEE
HPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGI
DDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRR
YLSENAHKAGADINVLEHALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=24.025974025974, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=100, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=25.8964143426295, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=310, Percent_Identity=22.9032258064516, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920448, Length=310, Percent_Identity=22.9032258064516, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38728; Mature: 38728

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEM
CCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEH
VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDR
HHHCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
VQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG
HCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
CEEEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGH
HHHHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
ITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEM
CCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEEH
VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDR
HHHCEEEECCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHH
VQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG
HCCCCEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH
CEEEEEEEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHH
LQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGH
HHHHCCHHCCHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
ITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]