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The map label for this gene is aceB [H]

Identifier: 157163479

GI number: 157163479

Start: 4244634

End: 4246235

Strand: Direct

Name: aceB [H]

Synonym: EcHS_A4248

Alternate gene names: 157163479

Gene position: 4244634-4246235 (Clockwise)

Preceding gene: 157163478

Following gene: 157163480

Centisome position: 91.41

GC content: 51.25

Gene sequence:

>1602_bases
ATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCATAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGC
CGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGC
AGCAGCAAGATATTGATAACGGAACTTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATACGGACTGGAAAATT
CGTGGTATTCCCGCGGACTTACAAGATCGTCGAGTCGAGATAACTGGCCCGGTTGAGCGCAAGATGGTGATCAACGCACT
GAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAA
TTAACCTGCGCGATGCGGTTAACGGCACCATCAGCTATACCAATGAAGCAGGCAAAATTTATCAGCTCAAGCCCAATCCA
GCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGTAG
CCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGCGGTCCCTATTTCTATCTGC
CGAAAACCCAGTCCTGGCAGGAAGCGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGC
GGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGA
CCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCG
TCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCAT
AAACGCGGTGCTTTTGCGATGGGCGGCATGGCAGCGTTTATTCCGAGCAAAGATGAAGAGCGCAATAACCAGGTGCTCAA
CAAAGTAAAAGCGGATAAAGCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATTGCTCACCCAGGCCTTGCGGATA
CGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGTGCCGATT
ACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCAGTGCA
GTACATCGAAGCATGGATCTCCGGCAACGGCTGCGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT
CCCGTACCTCAATCTGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCAAAGCCTTGTTCCGC
CAGATGCTGGGTGAAGAGATGAAAGTCATTGCCAGCGAACTGGGCGAAGAACGTTTCTCCCAGGGGCGTTTTGACGATGC
CGCACGCTTGATGGAACAGATCACCACTTCCGATGAGTTAATTGATTTCCTGACCCTGCCAGGCTACCGCCTGTTAGCGT
AA

Upstream 100 bases:

>100_bases
CATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGCGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGAT
CCATGACGAGGAGCTGCACG

Downstream 100 bases:

>100_bases
ACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAA
CCGCGTTGGGAAGGCATTAC

Product: malate synthase

Products: NA

Alternate protein names: MSA [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKI
RGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCH
KRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDVPI
TADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA

Sequences:

>Translated_533_residues
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKI
RGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCH
KRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDVPI
TADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
>Mature_532_residues
TEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIR
GIPADLQDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPA
VLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPRG
TIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHK
RGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDVPIT
ADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFRQ
MLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=533, Percent_Identity=98.874296435272, Blast_Score=1102, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17561814, Length=516, Percent_Identity=51.1627906976744, Blast_Score=508, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71982926, Length=410, Percent_Identity=53.9024390243902, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=47.4708171206226, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6324212, Length=528, Percent_Identity=46.0227272727273, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60346; Mature: 60215

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
GTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFED
CCCCHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
CCCCCHHHHCCCCEEEHHHCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHCCCC
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
CCCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCE
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNG
EECCCHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDVPITADQLLAPCDGERTEEGMRA
CCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCC
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
TEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
GTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKMVINALNANVKVFMADFED
CCCCHHHHHHCCCCCCCEEEECCCCCCCCCEEEEECCHHHHHHHHHHCCCEEEEEECCCC
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
CCCCCHHHHCCCCEEEHHHCCCEEEEECCCCEEEEECCCCEEEEEEECCCCCCCHHCCCC
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
CCCCCCHHHHHHHHHHHCCHHEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCE
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEERNNQVLNKVKADKALEANNG
EECCCHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDVPITADQLLAPCDGERTEEGMRA
CCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCC
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3050899; 3060852; 8265357; 9278503 [H]