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The map label for this gene is mtlA

Identifier: 157163078

GI number: 157163078

Start: 3798811

End: 3800724

Strand: Direct

Name: mtlA

Synonym: EcHS_A3806

Alternate gene names: 157163078

Gene position: 3798811-3800724 (Clockwise)

Preceding gene: 157163077

Following gene: 157163079

Centisome position: 81.81

GC content: 53.76

Gene sequence:

>1914_bases
ATGTCATCCGATATTAAGATCAAAGTGCAAAGCTTTGGTCGTTTCCTCAGCAACATGGTGATGCCAAATATCGGCGCGTT
TATCGCGTGGGGTATCATCACCGCATTATTTATTCCAACAGGGTGGTTACCGAACGAGACGCTGGCGAAGCTAGTCGGAC
CGATGATCACTTACCTCCTGCCGCTGCTGATCGGTTATACCGGTGGTAAGCTGGTAGGCGGCGAACGTGGCGGCGTAGTC
GGTGCCATCACCACCATGGGCGTTATCGTCGGCGCAGACATGCCGATGTTCCTCGGTTCTATGATTGCAGGTCCGCTGGG
CGGCTGGTGCATTAAGCACTTCGACCGCTGGGTAGACGGTAAGATCAAATCCGGTTTTGAGATGCTGGTGAATAACTTCT
CCGCTGGCATCATCGGGATGATCCTCGCCATTCTGGCATTCCTCGGCATTGGCCCGATTGTTGAAGCCCTGTCCAAAATG
CTGGCTGCGGGCGTTAACTTCATGGTTGTCCATGACATGCTGCCACTGGCATCTATCTTTGTTGAACCGGCGAAAATCCT
GTTCCTCAACAACGCCATTAACCACGGTATCTTCTCGCCGCTGGGTATTCAGCAGTCCCATGAACTGGGTAAATCCATCT
TCTTCCTGATTGAAGCTAACCCAGGTCCAGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGTGCTAAA
CAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGTGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCC
GCGTCTGATCCTGGCCGTTATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGGCGGTGGTCTGGTTTCTC
CAGCATCTCCGGGGTCTATCCTTGCGGTACTGGCGATGACGCCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGT
GCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGC
AGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTGACTAACGACC
TGAGCCACGTACGTAAAATCATCGTTGCCTGTGACGCCGGTATGGGTTCCAGTGCGATGGGCGCAGGCGTTCTGCGTAAG
AAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGT
CATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCG
ACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGAC
AGCCTGAAAGACAGCTTTGACGATTCCAGTGCTAACCTGTTCAAGCTAGGCGCGGAGAACATCTTCCTCGGTCGCAAAGC
GGCAACCAAAGAAGAAGCGATTCGTTTTGCTGGCGAGCAGCTGGTGAAAGGCGGTTACGTTGAGCCGGAATACGTTCAGG
CGATGCTGGATCGTGAAAAACTGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAA
GATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGC
CCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATG
AGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAA

Upstream 100 bases:

>100_bases
TTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCTACATTAACAAAAAACCTCGGGCTTCCAGCCTGCGCGACAGCAA
ACATAAGAAGGGGTGTTTTT

Downstream 100 bases:

>100_bases
TCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATC
ACAATTTGTTGAATTTGCAC

Product: PTS system, mannitol-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component; Mannitol-specific phosphotransferase enzyme IIA component; PTS system mannitol-specific EIIA component

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVV
GAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVC
AAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRK
KIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTANEEKVKD
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAK
DRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK

Sequences:

>Translated_637_residues
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVV
GAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVC
AAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRK
KIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTANEEKVKD
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAK
DRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
>Mature_636_residues
SSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVG
AITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKML
AAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAKQ
SAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCA
AMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKK
IQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTANEEKVKDS
LKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKD
RVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1790027, Length=637, Percent_Identity=100, Blast_Score=1292, Evalue=0.0,
Organism=Escherichia coli, GI1789301, Length=469, Percent_Identity=52.6652452025586, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1788494, Length=145, Percent_Identity=46.2068965517241, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTM3C_ECOLI (P00550)

Other databases:

- EMBL:   V01503
- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X06794
- PIR:   A00661
- RefSeq:   AP_004193.1
- RefSeq:   NP_418056.1
- PDB:   1A3A
- PDB:   1J6T
- PDB:   1VKR
- PDB:   1VRV
- PDB:   2FEW
- PDBsum:   1A3A
- PDBsum:   1J6T
- PDBsum:   1VKR
- PDBsum:   1VRV
- PDBsum:   2FEW
- ProteinModelPortal:   P00550
- DIP:   DIP-10267N
- IntAct:   P00550
- MINT:   MINT-245984
- STRING:   P00550
- EnsemblBacteria:   EBESCT00000000300
- EnsemblBacteria:   EBESCT00000015432
- GeneID:   948118
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3573
- KEGG:   eco:b3599
- EchoBASE:   EB0610
- EcoGene:   EG10615
- eggNOG:   COG2213
- GeneTree:   EBGT00050000009675
- HOGENOM:   HBG296680
- OMA:   GMIMPNI
- ProtClustDB:   PRK15083
- BioCyc:   EcoCyc:MTLA-MONOMER
- Genevestigator:   P00550
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718
- Gene3D:   G3DSA:3.40.930.10
- TIGRFAMs:   TIGR00851

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB; SSF55804 PTrfase/Anion_transptr

EC number: =2.7.1.69

Molecular weight: Translated: 67973; Mature: 67842

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 384-384 ACT_SITE 554-554

Signals:

None

Transmembrane regions:

HASH(0x18554628)-; HASH(0x16dea16c)-; HASH(0x1880c644)-; HASH(0x1880decc)-; HASH(0x16b326ec)-; HASH(0x18813a04)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLL
CCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDG
HHHHCCCCCEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
KIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
HCCHHEEEECCHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSI
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQISVTNSAINNL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHCC
PPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTANEEKVKD
CCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
HHHHCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEH
CCHHHCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCH
IQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCH
>Mature Secondary Structure 
SSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVGPMITYLL
CCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
PLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAGPLGGWCIKHFDRWVDG
HHHHCCCCCEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
KIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAGVNFMVVHDMLPLASIF
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPGMGVLLAYMFFGRGSAK
HCCHHEEEECCHHHCCCCCCCCCCHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCC
QSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTLTILGGGLVSPASPGSI
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
LAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDDIEAATRRMQDMKAESK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQDAGLSQISVTNSAINNL
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHCC
PPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTERLVAAQRHTANEEKVKD
CCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
SLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
HHHHCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEH
CCHHHCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCH
IQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK
HHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6309813; 8041620; 9278503; 3135464; 3142516; 1946374; 9551558