Click here to switch to the map view.

The map label for this gene is malS

Identifier: 157163048

GI number: 157163048

Start: 3766800

End: 3768830

Strand: Direct

Name: malS

Synonym: EcHS_A3774

Alternate gene names: 157163048

Gene position: 3766800-3768830 (Clockwise)

Preceding gene: 157163046

Following gene: 157163049

Centisome position: 81.12

GC content: 52.83

Gene sequence:

>2031_bases
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGC
CTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTG
ACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCAT
CCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCA
GCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATG
TTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCA
TAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAAAACGGCGATCCCAGTAATGACCAGAGTTACGGACGTCATA
AAGACGGTATGGCGGAAATTGGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAG
TTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTT
CCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTAC
GGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACG
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAAGTGAAAAAATCGCTGGGTGAACGCTG
GAGCGACTGGAAACCTGCCGCCGGGCAAACCTGGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGG
ATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCG
CTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATAC
CCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGA
TTGATGGTTTTCGGGTCGATACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCG
CTTCGCGAATGGAAAAAAGCTAACCCCGACAAAGCATTAGATGACAAACCTTTCTGGATGACCGGTGAAGCCTGGGGCCA
CGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAG
CAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGT
ACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATA
TGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACTGGCAGAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGCGGGCAAACAAACGACACTTTTGCTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAA
AGTGCTGGTCGTCTGGGCAGGGCAACAGTAA

Upstream 100 bases:

>100_bases
CCTTGCTTCTCATCCTGTGGACTTACCGCTCAGGGATGAGTTTTGTTTGGCTTATCGCTGGCAAACTGTCTGAAATCGCA
GCAATAAGGACTCATCCGCC

Downstream 100 bases:

>100_bases
CTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGA
TAAAGCACCGCTCACTCTTC

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ

Sequences:

>Translated_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ
>Mature_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSY
LSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=100, Blast_Score=1405, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=143, Percent_Identity=30.0699300699301, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMY1_ECOLI (P25718)

Other databases:

- EMBL:   X58994
- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S23807
- RefSeq:   AP_004221.1
- RefSeq:   NP_418028.1
- ProteinModelPortal:   P25718
- SMR:   P25718
- DIP:   DIP-10148N
- MINT:   MINT-1293484
- STRING:   P25718
- EnsemblBacteria:   EBESCT00000002245
- EnsemblBacteria:   EBESCT00000016789
- GeneID:   948088
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3543
- KEGG:   eco:b3571
- EchoBASE:   EB1292
- EcoGene:   EG11316
- eggNOG:   COG0366
- GeneTree:   EBGT00050000009459
- HOGENOM:   HBG298608
- OMA:   RISQFRA
- ProtClustDB:   PRK09505
- BioCyc:   EcoCyc:ALPHA-AMYL-PERI-MONOMER
- BioCyc:   MetaCyc:ALPHA-AMYL-PERI-MONOMER
- Genevestigator:   P25718
- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80
- PIRSF:   PIRSF036917
- SMART:   SM00642

Pfam domain/function: PF00128 Alpha-amylase; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.1

Molecular weight: Translated: 75713; Mature: 75713

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: ACT_SITE 460-460 ACT_SITE 503-503

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHH
CPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIMLQPAATSNGLLLLERAETDT
CCCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCEE
YKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHH
REHGDDKVLVVWAGQQ
HHCCCCEEEEEECCCC
>Mature Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPTLMISIQNAAEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHH
CPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIMLQPAATSNGLLLLERAETDT
CCCCCCCEEEEEEECCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGNEADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCHHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDEVKKSLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCEE
YKNKMDTHAKAIDGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANPDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQGFNVLSYLSSHDTRLFREGGDKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHH
REHGDDKVLVVWAGQQ
HHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356