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The map label for this gene is mdtE [H]

Identifier: 157162991

GI number: 157162991

Start: 3700543

End: 3701700

Strand: Direct

Name: mdtE [H]

Synonym: EcHS_A3715

Alternate gene names: 157162991

Gene position: 3700543-3701700 (Clockwise)

Preceding gene: 157162990

Following gene: 157162992

Centisome position: 79.69

GC content: 54.58

Gene sequence:

>1158_bases
ATGAACAGAAGAAGAAAGCTGTTAATACCGTTGTTATTCTGCGGCGCGATGCTCACCGCCTGCGATGACAAATCGGCGGA
AAACGCCGCCGCCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCG
GTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGC
GATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTC
GCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGAAGACCAACTACG
TTAGCCGTCAGGATTACGACACCGCGCGCACCCAGTTGAATGAAGCAGAAGCCAATGTCACCGTCGCCAAAGCGGCTGTT
GAACAGGCGACGATCAATCTGCAATACGCGAATGTCACCTCGCCGATTACGGGCGTCAGCGGGAAATCGTCGGTGACCGT
CGGCGCACTCGTTACCGCTAATCAGGCAGATTCGCTGGTTACCGTACAACGTCTGGACCCGATTTATGTCGATCTCACGC
AGTCGGTGCAAGATTTCTTACGCATGAAAGAAGAGGTCGCCAGTGGGCAAATCAAACAGGTTCAGGGCAGTACGCCAGTA
CAGCTCAATCTGGAAAATGGTAAACGCTACAGCCAGACCGGCACGCTGAAATTCTCCGACCCGACAGTGGATGAAACCAC
GGGCTCCGTGACGTTACGGGCGATTTTCCCCAACCCAAATGGTGACTTGCTGCCTGGCATGTACGTCACGGCATTAGTGG
ATGAAGGTAGCCGCCAGAATGTATTACTGGTGCCGCAGGAAGGCGTCACCCACAACGCCCAGGGTAAAGCAACGGCGCTC
ATTCTGGATAAAGACGATGTCGTGCAGCTACGCGAAATTGAAGCCAGCAAAGCCATCGGCGACCAGTGGGTCGTCACCTC
TGGCTTGCAGGCTGGCGATCGGGTGATCGTTTCCGGTTTGCAACGCATTCGTCCGGGTATCAAAGCACGAGCAATTTCCT
CCAGCCAGGAAAACGCCAGCACCGAATCGAAACAATAA

Upstream 100 bases:

>100_bases
GCTGACAACCCCACTATGCCGCTGGTCTGTAAATCCCTCATATCTCTCCTCGCGCGCAATTTAAAGAACCGTTATTTCTC
AAGAATTTTCAGGGACTAAA

Downstream 100 bases:

>100_bases
CGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTAT
GATGCTTGCAGGTGGTCTGG

Product: multidrug efflux system protein MdtE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ

Sequences:

>Translated_385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ
>Mature_385_residues
MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEG
DKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATAL
ILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=100, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1786668, Length=382, Percent_Identity=54.4502617801047, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789665, Length=382, Percent_Identity=49.4764397905759, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI87082036, Length=311, Percent_Identity=28.6173633440514, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081796, Length=346, Percent_Identity=24.2774566473988, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081951, Length=130, Percent_Identity=35.3846153846154, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41191; Mature: 41191

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCH
VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARI
HHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEE
TFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVS
EECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHEEEEEEEECCCCCCCCCC
GKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
CCCCEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQN
EEEECCCCEECCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
VLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGL
EEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
QRIRPGIKARAISSSQENASTESKQ
HHHCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCCCCH
VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARI
HHHCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEE
TFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVS
EECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHEEEEEEEECCCCCCCCCC
GKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPV
CCCCEEEEEEEECCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCE
QLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQN
EEEECCCCEECCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
VLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGL
EEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
QRIRPGIKARAISSSQENASTESKQ
HHHCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]