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The map label for this gene is acpT [H]

Identifier: 157162952

GI number: 157162952

Start: 3659239

End: 3659826

Strand: Direct

Name: acpT [H]

Synonym: EcHS_A3674

Alternate gene names: 157162952

Gene position: 3659239-3659826 (Clockwise)

Preceding gene: 157162951

Following gene: 157162953

Centisome position: 78.8

GC content: 56.46

Gene sequence:

>588_bases
ATGTATCGGATAGTTCTGGGGAAAGTTTCGACCTTAAGCGCAGCTCCACTGCCACCGGGTTTACGCGAGCAAGCACCGCA
AGGTCCACGACGCGAACGCTGGCTGGCGGGGCGTGCATTGCTTTCGCACACGCTTTCCCCGCTACCGGAGATCATCTATG
GCGAACAAGGCAAACCTGCATTTGCGCCGGAAATGCCGCTATGGTTCAACTTAAGCCATAGCGGTGACGATATCGCCCTG
CTGTTGAGTGATGAAGGCGAAGTCGGCTGCGATATCGAAGTGATTCGCCCGCGCGCCAACTGGCGCTGGCTGGCGAACGC
CGTATTCAGCCTCGGGGAACACGCTGAGATGGACGCCGTGCATCCTGATCAGCAACTGGAAATGTTCTGGCGCATCTGGA
CGCGCAAAGAAGCCATCGTTAAACAGCGTGGCGGCAGCGCCTGGCAAATCGTCAGCGTAGACAGCACCTATCACTCCTCG
CTGTCAGTCAGCCATTGTCAGCTTGAAAATTTAAGCCTTGCGATCTGCACCCCTACTCCCTTTACGCTCACCGCCGACAG
TGTGCAATGGATCGATTCAGTTAACTGA

Upstream 100 bases:

>100_bases
GATTTTACTGGGGCCGGGCAGACCGAAAAGTCGGTTACCGGGATGAGGCGACAAGTGATACGATACGCACTTTCATTTTC
CATTAAACGTTGGCCCTGAT

Downstream 100 bases:

>100_bases
TCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTC
CGCCATCTATTTTAATCCAT

Product: holo-(acyl carrier protein) synthase 2

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Sequences:

>Translated_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN
>Mature_195_residues
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIAL
LLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN

Specific function: May be involved in an alternative pathway for phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The native apo-protein substrate is unknown. Is able to functionally replace AcpS in vivo but only when expressed at high levels [H]

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Escherichia coli, GI1789886, Length=195, Percent_Identity=100, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 21768; Mature: 21768

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCHHHHCCCC
>Mature Secondary Structure
MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHTLSPLPEIIYGEQGKPA
CEEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
FAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWRWLANAVFSLGEHAEMDAV
CCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
HPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSSLSVSHCQLENLSLAICTPTP
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECEEEECCCEEEEECCCC
FTLTADSVQWIDSVN
CEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]