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The map label for this gene is gntU

Identifier: 157162914

GI number: 157162914

Start: 3622757

End: 3624097

Strand: Reverse

Name: gntU

Synonym: EcHS_A3635

Alternate gene names: 157162914

Gene position: 3624097-3622757 (Counterclockwise)

Preceding gene: 157162915

Following gene: 157162912

Centisome position: 78.05

GC content: 55.48

Gene sequence:

>1341_bases
GTGACTACATTAACGCTTGTTTTAACAGCAGTAGGGTCTGTTTTACTGCTGCTGTTTTTAGTCATGAAGGCGCGTATGCA
CGCTTTCCTGGCTTTAATGGTGGTGTCCATGGGGGCTGGCCTTTTTTCCGGTATGCCGCTCGATAAAATCGCAGCGACGA
TGGAAAAAGGGATGGGAGGCACCCTCGGCTTCCTGGCGGTGGTTGTCGCCCTGGGAGCTATGTTTGGCAAGATCTTACAT
GAAACCGGCGCAGTCGATCAGATTGCCGTCAAAATGCTCAAATCCTTCGGTCACAGCCGCGCGCATTATGCCATCGGCCT
TGCGGGGCTGGTCTGTGCGCTACCGCTGTTCTTTGAAGTGGCGATTGTTCTGCTGATTAGCGTTGCTTTCTCAATGGCGC
GCCACACCGGTACGAACCTGGTGAAGCTGGTAATCCCATTATTCGCAGGCGTGGCGGCAGCGGCGGCGTTCCTGGTGCCA
GGGCCAGCGCCAATGCTGCTGGCATCGCAGATGAACGCCGATTTTGGCTGGATGATCCTGATTGGCCTGTGTGCGGCAAT
TCCGGGAATGATTATTGCCGGGCCGCTGTGGGGTAATTTCATCAGCCGTTACGTGGAGTTGCATATTCCTGACGACATCA
GCGAACCGCATCTCGGCGAAGGCAAAATGCCGTCCTTCGGATTCAGCCTGTCGCTGATCCTGTTGCCGCTGGTGCTGGTG
GGGCTGAAAACCATTGCCGCGCGTTTTGTGCCAGAAGGCTCTACCGCTTACGAATGGTTCGAGTTTATTGGTCATCCGTT
TACCGCGATTCTGGTTGCTTGTCTGGTGGCGATTTATGGCCTGGCGATGCGTCAGGGCATGCCAAAAGACAAAGTGATGG
AGATTTGCGGTCACGCGCTGCAACCGGCGGGGATCATTCTGCTGGTGATTGGTGCGGGCGGCGTGTTCAAACAGGTGCTG
GTTGACTCTGGCGTAGGTCCGGCACTGGGCGAAGCGTTAACCGGCATGGGCCTGCCGATTGCTATCACCTGCTTCGTGCT
GGCCGCTGCAGTGCGCATCATTCAGGGGTCTGCCACCGTAGCCTGTTTAACGGCGGTGGGACTGGTGATGCCAGTCATTG
AACAACTGAACTACTCTGGTGCGCAAATGGCGGCACTGTCGATTTGTATCGCTGGTGGTTCGATTGTTGTCAGCCACGTT
AACGACGCTGGTTTCTGGTTGTTCGGTAAATTTACCGGCGCGACCGAAGCCGAAACGCTGAAAACCTGGACCATGATGGA
AACCATTCTCGGCACTGTCGGTGCCATCGTTGGGATGATTGCGTTCCAGCTGTTGAGTTAA

Upstream 100 bases:

>100_bases
GGGTGCGGACGAAACCGATGTACTGGTGGTGGATATCGATCAACCGCTGGAAGGTGTTGTGGCAAGCACCATTGAGGTTA
TTAAAAAAGGCAAATAAGTA

Downstream 100 bases:

>100_bases
GTTTGTTCGCCCGGTAGTTGTGACGCTACCGGGTTCTTTTCGAAAAACTCTCCTCGTTACCCCTTCATCCACATTCGAAT
GCCGTCGAGGAACATCTGGG

Product: low affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILH
ETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVL
VDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHV
NDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS

Sequences:

>Translated_446_residues
MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILH
ETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVL
VDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHV
NDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS
>Mature_445_residues
TTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHE
TGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPG
PAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLVG
LKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLV
DSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVN
DAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS

Specific function: Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family

Homologues:

Organism=Escherichia coli, GI48994939, Length=446, Percent_Identity=100, Blast_Score=875, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=430, Percent_Identity=37.2093023255814, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI48994935, Length=434, Percent_Identity=36.405529953917, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI2367370, Length=429, Percent_Identity=33.5664335664336, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789097, Length=450, Percent_Identity=32.6666666666667, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788707, Length=429, Percent_Identity=35.4312354312354, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1790776, Length=431, Percent_Identity=30.8584686774942, Blast_Score=204, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GNTU_ECO57 (P0AC97)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E86010
- PIR:   E91164
- RefSeq:   NP_289984.1
- RefSeq:   NP_312312.1
- ProteinModelPortal:   P0AC97
- EnsemblBacteria:   EBESCT00000026621
- EnsemblBacteria:   EBESCT00000060368
- GeneID:   915858
- GeneID:   961623
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4804
- KEGG:   ecs:ECs4285
- GeneTree:   EBGT00050000009137
- HOGENOM:   HBG649941
- OMA:   VIQGSAT
- ProtClustDB:   PRK10472
- BioCyc:   ECOL83334:ECS4285-MONOMER
- InterPro:   IPR003474
- TIGRFAMs:   TIGR00791

Pfam domain/function: PF02447 GntP_permease

EC number: NA

Molecular weight: Translated: 46417; Mature: 46285

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17fdb174)-; HASH(0x17a090bc)-; HASH(0x181090e4)-; HASH(0x182552fc)-; HASH(0x17e13354)-; HASH(0x17b96170)-; HASH(0x17723b04)-; HASH(0x18147f64)-; HASH(0x1828297c)-; HASH(0x175717b4)-; HASH(0x17f786a8)-; HASH(0x17eb7818)-; HASH(0x17ddf93c)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCC
TLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMIL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHH
IGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
HHHHHHCCHHHEECHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHAL
HHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV
CCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHH
ACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCHHHH
KTWTMMETILGTVGAIVGMIAFQLLS
HHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796