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The map label for this gene is glgX [H]

Identifier: 157162910

GI number: 157162910

Start: 3616383

End: 3618356

Strand: Reverse

Name: glgX [H]

Synonym: EcHS_A3631

Alternate gene names: 157162910

Gene position: 3618356-3616383 (Counterclockwise)

Preceding gene: 157162911

Following gene: 157162909

Centisome position: 77.92

GC content: 54.15

Gene sequence:

>1974_bases
ATGACACAACTCGCCATTGGCAAACCCGCTCCCCTCGGCGCGCATTACGACGGTCAGGGCGTCAACTTCACACTTTTCTC
CGTTCATGCCGAGCGGGTAGAACTGTGTGTCTTTGACGCCAATGGCCAGGAACATCGCTATGACTTGCCAGGGCACAGTG
GCGACATTTGGCACGGTTATCTGCCGGATGCGCGCCCGGGTTTGCGTTATGGTTATCGCGTTCATGGCCCCTGGCAACCC
GCCGAGGGGCATCGCTTTAACCCGGCGAAGTTGTTGATTGATCCTTGCGCGCGGCAAATTGACGGGGAGTTTAAAGATAA
CCCGCTGCTGCACGCCGGTCATAATGAACCTGACTATCGCGACAACGCCGCCATTGCGCCGAAATGCGTAGTGGTGGTTG
ATCACTATGACTGGGAAGATGATGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGA
TTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTA
TTTGAAACAATTGGGCATTACCGCGCTGGAACTGCTGCCAGTGGCGCAGTTTGCCAGTGAACCACGTCTGCAACGCATGG
GGCTAAGTAACTACTGGGGTTACAACCCGGTGGCGATGTTTGCGCTGCATCCGGCGTATGCCTGCTCGCCAGAAACGGCG
CTGGATGAGTTTCGCGATGCAATCAAAGCACTGCATAAAGCGGGTATCGAAGTCATTCTTGATATCGTGCTCAACCATAG
TGCGGAACTGGACCTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACG
GCGATTATCACAACTGGACCGGTTGCGGCAACACGCTCAATTTGAGTCATCCGGCGGTGGTGGATTATGCCAGCGCCTGC
CTGCGTTATTGGGTAGAAACCTGCCACGTCGATGGTTTCCGCTTTGATCTGGCGGCAGTCATGGGCCGTACGCCAGAGTT
CCGTCAGGATGCGCCGTTGTTTACCGCTATCCAGAACTGCCCGGTGCTCTCGCAGGTGAAGTTAATTGCTGAACCGTGGG
ATATCGCTCCTGGTGGTTATCAGGTGGGAAATTTCCCGCCGCTGTTTGCCGAGTGGAACGATCATTTCCGCGATGCTGCC
CGTCGTTTCTGGCTGCATTATGATTTGCCTCTGGGGGCGTTTGCCGGGCGTTTTGCTGCCTCCAGCGATGTTTTTAAACG
TAATGGTCGTCTGCCGAGTGCCGCGATTAATCTCGTCACCGCACATGACGGTTTTACGCTTCGCGACTGCGTTTGCTTCA
ACCATAAACACAATGAAGCAAACGGAGAAGAAAATCGCGACGGGACCAACAACAATTACAGTAACAATCATGGTAAAGAA
GGGTTAGGTGGTACTCTTGATCTGGTTGAACGGCGGCGCGACAGCATTCATGCCCTGTTAACAACGTTGTTGCTCTCCCA
GGGCACGCCGATGTTACTGGCCGGTGACGAACATGGTCACAGCCAGCATGGCAATAACAATGCCTACTGTCAGGATAACC
AATTAACCTGGTTGGACTGGTCGCAGGCAAGCAGTGGTTTAACCGCATTTACCGCCGCGTTAATCCATCTGCGCAAGCGT
ATTCCCGCTTTGGTGGAGAATCGCTGGTGGGAAGAAGGCGACGGCAATGTCCGTTGGCTAAATCGATATGCTCAACCTTT
AAGCACGGATGAGTGGCAAAACGGGCCGAAACAGCTGCAAATTCTGCTCTCGGATCGCTTTTTGATCGCAATTAACGCCA
CGCTTGAGGTAACAGAGATTGTTTTACCTGCTGGGGAGTGGCACGCCATTCCCCCATTCGCTGGAGAGGATAACCCAGTG
ATTACGGCTGTCTGGCAGGGACCTGCACACGGATTGTGTGTGTTCCAGAGATGA

Upstream 100 bases:

>100_bases
GCACGGTACACAGCGATGAGATTGCCAGCCACGGTCGTCAGCATTCACTAAGCCTGACGCTACCACCGCTGGCCACTATC
TGGCTGGTTCGGGAGGCAGA

Downstream 100 bases:

>100_bases
TAAAAAAGGAGTTAGTCATGGTTAGTTTAGAGAAGAACGATCACTTAATGTTGGCGCGCCAGCTGCCATTGAAATCTGTT
GCCCTGATACTGGCGGGAGG

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MTQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQEHRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQP
AEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETA
LDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASAC
LRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAA
RRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKE
GLGGTLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKR
IPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPV
ITAVWQGPAHGLCVFQR

Sequences:

>Translated_657_residues
MTQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQEHRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQP
AEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETA
LDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASAC
LRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAA
RRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKE
GLGGTLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKR
IPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPV
ITAVWQGPAHGLCVFQR
>Mature_656_residues
TQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQEHRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPA
EGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGL
TYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETAL
DEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACL
RYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAAR
RFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKEG
LGGTLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRI
PALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPVI
TAVWQGPAHGLCVFQR

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=99.6955859969559, Blast_Score=1358, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73619; Mature: 73488

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQEHRYDLPGHSGDIWHGY
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCCCCEEECCCCCCCEEECC
LPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
DNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTYLHPEIPVEIRGTYKALG
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEECCHHHHHC
HPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHH
LDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCC
GCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNC
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCC
PVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAARRFWLHYDLPLGAFAGRFAA
CCHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCC
SSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKE
CCHHHHHCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGTLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEH
SQASSGLTAFTAALIHLRKRIPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCHHHCCCHHHEE
ILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR
EEEECCEEEEEECEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECC
>Mature Secondary Structure 
TQLAIGKPAPLGAHYDGQGVNFTLFSVHAERVELCVFDANGQEHRYDLPGHSGDIWHGY
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECEEEEEEECCCCCCCEEECCCCCCCEEECC
LPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDGEFKDNPLLHAGHNEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
DNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKGLTYLHPEIPVEIRGTYKALG
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEECCHHHHHC
HPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNYWGYNPVAMFALHPAYACSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHH
LDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPLFSLRGIDNRSYYWIREDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCC
GCGNTLNLSHPAVVDYASACLRYWVETCHVDGFRFDLAAVMGRTPEFRQDAPLFTAIQNC
CCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCC
PVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEWNDHFRDAARRFWLHYDLPLGAFAGRFAA
CCHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCC
SSDVFKRNGRLPSAAINLVTAHDGFTLRDCVCFNHKHNEANGEENRDGTNNNYSNNHGKE
CCHHHHHCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGTLDLVERRRDSIHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEH
SQASSGLTAFTAALIHLRKRIPALVENRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCHHHCCCHHHEE
ILLSDRFLIAINATLEVTEIVLPAGEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR
EEEECCEEEEEECEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA