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The map label for this gene is glpR [H]

Identifier: 157162900

GI number: 157162900

Start: 3604117

End: 3604875

Strand: Reverse

Name: glpR [H]

Synonym: EcHS_A3620

Alternate gene names: 157162900

Gene position: 3604875-3604117 (Counterclockwise)

Preceding gene: 157162901

Following gene: 157162898

Centisome position: 77.63

GC content: 54.55

Gene sequence:

>759_bases
ATGAAACAAACACAACGTCACAACGGTATTATCGAACTGGTTAAACAGCAGGGTTATGTCAGTACCGAAGAGCTGGTAGA
GCATTTCTCCGTCAGCCCGCAGACTATTCGCCGCGACCTCAATGAGCTGGCGGAGCAAAACCTGATCCTGCGCCATCATG
GCGGTGCGGCGCTGCCTTCCAGTTCGGTTAACACGCCGTGGCACGATCGCAAGGCCACCCAGACCGAAGAAAAAGAGCGC
ATCGCCCGCAAAGTGGCGGAGCAAATCCCCAATGGCTCGACGCTGTTTATCGATATCGGCACCACGCCGGAAGCGGTAGC
GCACGCACTGCTCAATCACAGCAATTTGCGCATTGTCACCAACAATCTCAACGTTGCTAACACGTTGATGGTAAAAGAAG
ATTTTCGCATCATTCTCGCCGGTGGCGAATTACGCAGCCGCGATGGCGGGATCATTGGCGAAGCGACGCTCGATTTTATC
TCCCAGTTCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCA
CGAAGTTCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTA
ACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCAGCCGCCAGTAAGCGTGATGCAG
GTGCTGACGGACCACCATATTCAACTGGAGCTGTGCTGA

Upstream 100 bases:

>100_bases
GCGAACGGAGCACACATCGCCGGGTTAGCCGTGGGTTTAGCGATGGCTTTTGTTGATTCGCTCAATGCGCGAAAACGAAA
ATAATTCCAGGGATTTATAA

Downstream 100 bases:

>100_bases
TCCTGCAATGCTTCCCAGGTCAGACCAAAACGCGCCAGGTATTTGCGTAGCCGATCCGCGTCATTGACGCTGGCTTTGCC
CTGGCGCGAAACATCAAAAA

Product: DNA-binding transcriptional repressor GlpR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAQPPVSVMQ
VLTDHHIQLELC

Sequences:

>Translated_252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAQPPVSVMQ
VLTDHHIQLELC
>Mature_252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAQPPVSVMQ
VLTDHHIQLELC

Specific function: Repressor of the glycerol-3-phosphate regulon [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=252, Percent_Identity=99.6031746031746, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87082344, Length=246, Percent_Identity=44.7154471544715, Blast_Score=230, Evalue=8e-62,
Organism=Escherichia coli, GI1788069, Length=251, Percent_Identity=29.0836653386454, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789059, Length=251, Percent_Identity=29.8804780876494, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789519, Length=251, Percent_Identity=28.2868525896414, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI226510968, Length=250, Percent_Identity=30.4, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789170, Length=232, Percent_Identity=28.448275862069, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787063, Length=253, Percent_Identity=26.8774703557312, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790753, Length=253, Percent_Identity=24.1106719367589, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787540, Length=230, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790635, Length=247, Percent_Identity=24.6963562753036, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 28079; Mature: 28079

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPS
CCCCCHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
SSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
NNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFISQFRLDFGILGISGIDSDGS
CCCCEEEEEEEECCCEEEEECCCEECCCCCEEHHHHHHHHHHHHHHHEEEEEECCCCCCC
LLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAQPPVSVMQ
EEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHH
VLTDHHIQLELC
HHHCCCEEEEEC
>Mature Secondary Structure
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPS
CCCCCHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
SSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
NNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFISQFRLDFGILGISGIDSDGS
CCCCEEEEEEEECCCEEEEECCCEECCCCCEEHHHHHHHHHHHHHHHEEEEEECCCCCCC
LLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAQPPVSVMQ
EEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHH
VLTDHHIQLELC
HHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8955387; 3045764; 9278503 [H]