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The map label for this gene is envZ

Identifier: 157162881

GI number: 157162881

Start: 3578875

End: 3580227

Strand: Reverse

Name: envZ

Synonym: EcHS_A3601

Alternate gene names: 157162881

Gene position: 3580227-3578875 (Counterclockwise)

Preceding gene: 157162882

Following gene: 157162879

Centisome position: 77.1

GC content: 57.13

Gene sequence:

>1353_bases
ATGAGGCGATTGCGCTTCTCGCCACGAAGTTCATTTGCCCGTACGTTATTGCTCATCGTCACCTTGCTGTTCGCCAGCCT
GGTGACGACTTATCTGGTGGTGCTGAACTTCGCGATTTTGCCGAGCCTCCAGCAGTTTAATAAAGTCCTCGCGTACGAAG
TGCGTATGTTGATGACCGACAAACTGCAACTGGAGGACGGCACGCAGTTGGTTGTGCCTCCCGCTTTCCGTCGGGAGATC
TACCGTGAGCTGGGGATCTCTCTCTACTCCAACGAGGCTGCCGAAGAGGCAGGTCTGCGTTGGGCGCAACACTATGAATT
CTTAAGCCATCAGATGGCGCAGCAACTGGGCGGCCCGACGGAAGTGCGCGTTGAGGTCAACAAAAGTTCGCCTGTCGTCT
GGCTGAAAACCTGGCTGTCGCCCAATATCTGGGTACGCGTGCCGCTGACCGAAATTCATCAGGGCGATTTCTCTCCGCTG
TTCCGCTATACGCTGGCGATTATGCTATTGGCGATAGGCGGGGCGTGGCTGTTTATTCGTATCCAGAACCGACCGTTGGT
CGATCTCGAACACGCAGCCTTGCAGGTTGGTAAAGGGATTATTCCGCCGCCGCTGCGTGAGTATGGCGCGTCCGAGGTGC
GTTCCGTTACCCGTGCCTTTAACCATATGGCGGCTGGTGTTAAGCAACTGGCGGATGACCGCACGCTGCTGATGGCGGGG
GTAAGTCACGACTTGCGCACGCCGCTGACGCGTATTCGCCTGGCGACTGAGATGATGAGCGAGCAGGATGGCTATCTGGC
AGAATCGATCAATAAAGATATCGAAGAGTGCAACGCCATCATTGAGCAGTTTATCGACTACCTGCGCACCGGGCAGGAGA
TGCCGATGGAAATGGCGGATCTTAATGCAGTACTCGGTGAGGTGATTGCTGCCGAAAGTGGCTATGAGCGGGAAATTGAA
ACCGCGCTTTACCCCGGCAGCATTGAAGTGAAAATGCACCCGCTGTCGATCAAACGCGCGGTGGCGAATATGGTGGTCAA
CGCCGCCCGTTATGGCAATGGCTGGATCAAAGTCAGCAGCGGAACGGAGCCGAATCGCGCCTGGTTCCAGGTGGAAGATG
ACGGTCCGGGAATTGCGCCGGAACAACGTAAGCACCTGTTCCAGCCGTTTGTCCGCGGCGACAGTGCGCGCACCATTAGC
GGCACGGGATTAGGGCTGGCAATTGTGCAGCGTATCGTGGATAACCATAACGGGATGCTGGAGCTTGGCACCAGCGAGCG
GGGCGGGCTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTAACGCGGGCGCAGGGCACGACAAAAGAAGGGTAA

Upstream 100 bases:

>100_bases
CGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCGTCTGGGGTCTGGGCTACGTCTTT
GTACCGGACGGCTCTAAAGC

Downstream 100 bases:

>100_bases
ATAAACGGGAGGCGAAGGTGCCTCCCGTTTTGCTTTCTATAAGATACTGGATAGATATTCTCCAGCTTCAAATCATTACA
GTTTCGGACCAGCCGCTACC

Product: osmolarity sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG

Sequences:

>Translated_450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
>Mature_450_residues
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREI
YRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIE
TALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
GTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1789808, Length=450, Percent_Identity=100, Blast_Score=921, Evalue=0.0,
Organism=Escherichia coli, GI1787894, Length=265, Percent_Identity=32.4528301886792, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=30.2919708029197, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=26.605504587156, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790551, Length=270, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=291, Percent_Identity=24.3986254295533, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=31.3084112149533, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786783, Length=283, Percent_Identity=27.5618374558304, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=27.5229357798165, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=27.9352226720648, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789403, Length=248, Percent_Identity=27.0161290322581, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=210, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=240, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=26.1648745519713, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=251, Percent_Identity=23.5059760956175, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787374, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=217, Percent_Identity=27.6497695852535, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENVZ_ECOLI (P0AEJ4)

Other databases:

- EMBL:   J01656
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B25024
- RefSeq:   AP_004386.1
- RefSeq:   NP_417863.1
- PDB:   1BXD
- PDB:   1JOY
- PDB:   1NJV
- PDBsum:   1BXD
- PDBsum:   1JOY
- PDBsum:   1NJV
- ProteinModelPortal:   P0AEJ4
- SMR:   P0AEJ4
- DIP:   DIP-48357N
- IntAct:   P0AEJ4
- STRING:   P0AEJ4
- EnsemblBacteria:   EBESCT00000002321
- EnsemblBacteria:   EBESCT00000002322
- EnsemblBacteria:   EBESCT00000002323
- EnsemblBacteria:   EBESCT00000002324
- EnsemblBacteria:   EBESCT00000015113
- GeneID:   947272
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3367
- KEGG:   eco:b3404
- EchoBASE:   EB0265
- EcoGene:   EG10269
- eggNOG:   COG0642
- GeneTree:   EBGT00050000008662
- HOGENOM:   HBG334875
- OMA:   KPSYQQI
- ProtClustDB:   PRK09467
- BioCyc:   EcoCyc:ENVZ-MONOMER
- Genevestigator:   P0AEJ4
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 50335; Mature: 50335

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: BINDING 347-347

Signals:

None

Transmembrane regions:

HASH(0x170c9e18)-; HASH(0x17e0ff08)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
CEEECCCCEEEECHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
EEEEEECCCCCEEEEEECCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSS
HHHHHHHHHHCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECC
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCEE
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
EECCCCCCCEEEEEEECCCEECCCCCCCCH
>Mature Secondary Structure
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
CEEECCCCEEEECHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
EEEEEECCCCCEEEEEECCCCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEE
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
ECCCCCCHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSS
HHHHHHHHHHCCCCCCHHHHHEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECC
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCEE
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
EECCCCCCCEEEEEEECCCEECCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948