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The map label for this gene is dusB

Identifier: 157162741

GI number: 157162741

Start: 3454623

End: 3455588

Strand: Direct

Name: dusB

Synonym: EcHS_A3453

Alternate gene names: 157162741

Gene position: 3454623-3455588 (Clockwise)

Preceding gene: 157162740

Following gene: 157162742

Centisome position: 74.4

GC content: 52.28

Gene sequence:

>966_bases
ATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGAC
GTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAT
CTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCA
GATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGATTATTGATATCAATATGGGTTGCCCGGCTAAAAAAGTGAATCG
CAAGCTCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACG
TTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAACTGCGAAGAGATTGCCCAACTGGCTGAAGAC
TGTGGCATTCAGGCTCTGACCATTCATGGCCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCG
GGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCG
ACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTAT
CTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCA
TGACTTTTATGGTCCGGCAAAAGGGTACCGAATTGCACGTAAACACGTTTCCTGGTATCTCCAGGAACACGCTCCAAATG
ACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTT
GCGTAA

Upstream 100 bases:

>100_bases
ATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACA
GTATGGTCATTTCTTAACTC

Downstream 100 bases:

>100_bases
ACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTC
AGGATCAGGTAACCCAAAAA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENF
A

Sequences:

>Translated_321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENF
A
>Mature_321_residues
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMA
DAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENF
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=320, Percent_Identity=30.3125, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=30.7053941908714, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=31.6455696202532, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=31.0204081632653, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.2631578947368, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=100, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=33.6032388663968, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=29.7619047619048, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=199, Percent_Identity=33.6683417085427, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=266, Percent_Identity=30.4511278195489, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=29.7413793103448, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24585320, Length=244, Percent_Identity=31.5573770491803, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=31.7596566523605, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=31.7596566523605, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=33.0645161290323, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSB_ECO57 (P0ABT7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91145
- PIR:   H85990
- RefSeq:   NP_289828.1
- RefSeq:   NP_312159.1
- ProteinModelPortal:   P0ABT7
- SMR:   P0ABT7
- EnsemblBacteria:   EBESCT00000024493
- EnsemblBacteria:   EBESCT00000059334
- GeneID:   916020
- GeneID:   958697
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4620
- KEGG:   ecs:ECs4132
- GeneTree:   EBGT00050000009544
- HOGENOM:   HBG557545
- OMA:   QLAGCEP
- ProtClustDB:   PRK10415
- BioCyc:   ECOL83334:ECS4132-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00737

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 35867; Mature: 35867

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
IDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDV
ECCCCCEEEEECCCCHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
IDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDV
ECCCCCEEEEECCCCHHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAEDCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGYRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796