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The map label for this gene is ptsO

Identifier: 157162690

GI number: 157162690

Start: 3398893

End: 3399165

Strand: Direct

Name: ptsO

Synonym: EcHS_A3399

Alternate gene names: 157162690

Gene position: 3398893-3399165 (Clockwise)

Preceding gene: 157162689

Following gene: 157162691

Centisome position: 73.2

GC content: 49.08

Gene sequence:

>273_bases
ATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAAT
GCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGA
TGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATC
GCCCTCTTTAATTCTGGTTTTGATGAAGATTAA

Upstream 100 bases:

>100_bases
AGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGT
ACGCTGGAAAAACGTAAACC

Downstream 100 bases:

>100_bases
TCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCA
ACGTCATTTGTACATTTTGT

Product: phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr)

Products: NA

Alternate protein names: Nitrogen-related HPr

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI
ALFNSGFDED

Sequences:

>Translated_90_residues
MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI
ALFNSGFDED
>Mature_89_residues
TVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIA
LFNSGFDED

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the p

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1789599, Length=90, Percent_Identity=100, Blast_Score=181, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTSO_ECO57 (P0A9N2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91139
- PIR:   H85984
- RefSeq:   NP_289780.1
- RefSeq:   NP_312112.1
- ProteinModelPortal:   P0A9N2
- SMR:   P0A9N2
- EnsemblBacteria:   EBESCT00000024909
- EnsemblBacteria:   EBESCT00000055368
- GeneID:   916066
- GeneID:   958646
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4569
- KEGG:   ecs:ECs4085
- GeneTree:   EBGT00050000008931
- HOGENOM:   HBG653254
- OMA:   SVIAMLM
- ProtClustDB:   PRK10897
- BioCyc:   ECOL83334:ECS4085-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: NA

Molecular weight: Translated: 9810; Mature: 9679

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 16-16

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKG
CCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
RQIEVEATGPQEEEALAAVIALFNSGFDED
CEEEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKG
CCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
RQIEVEATGPQEEEALAAVIALFNSGFDED
CEEEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796