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The map label for this gene is yfiA

Identifier: 157162687

GI number: 157162687

Start: 3397100

End: 3397387

Strand: Direct

Name: yfiA

Synonym: EcHS_A3396

Alternate gene names: 157162687

Gene position: 3397100-3397387 (Clockwise)

Preceding gene: 157162686

Following gene: 157162688

Centisome position: 73.16

GC content: 44.79

Gene sequence:

>288_bases
ATGCAGCTCAACATTACCGGAAATAACGTCGAGATCACCGAGGCACTGCGCGAATTTGTTACAGCCAAATTTGCCAAACT
TGAGCAATATTTTGACCGAATCAACCAGGTCTATGTTGTTCTGAAAGTGGAGAAAGTCACCCACACCTCAGATGCAACAC
TGCATGTAAACGGTGGTGAAATTCATGCCAGCGCGGAAGGTCAGGACATGTACGCTGCCATTGATGGCTTAATTGATAAG
CTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAA

Upstream 100 bases:

>100_bases
GTGGCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGACC
CAACCGATAAGGAAGACACT

Downstream 100 bases:

>100_bases
TTGTCCGGGCAATTAGCATGTGCATGGCGGCCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGCTCCGAGCCTG
TTCCACTGTTTGAGTGGGCA

Product: putative sigma(54) modulation protein

Products: NA

Alternate protein names: ORF3; ORF95

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVEKVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDK
LARQLTKHKDKLKQH

Sequences:

>Translated_95_residues
MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVEKVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDK
LARQLTKHKDKLKQH
>Mature_95_residues
MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVEKVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDK
LARQLTKHKDKLKQH

Specific function: Probably modulates the activity of sigma(54) (rpoN)

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family

Homologues:

Organism=Escherichia coli, GI1789595, Length=95, Percent_Identity=100, Blast_Score=194, Evalue=9e-52,
Organism=Escherichia coli, GI1788949, Length=87, Percent_Identity=40.2298850574713, Blast_Score=69, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RP5M_ECO57 (P0AFX2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91139
- PIR:   E85984
- RefSeq:   NP_289777.1
- RefSeq:   NP_312109.1
- ProteinModelPortal:   P0AFX2
- SMR:   P0AFX2
- MINT:   MINT-1249557
- EnsemblBacteria:   EBESCT00000024236
- EnsemblBacteria:   EBESCT00000059995
- GeneID:   916070
- GeneID:   958642
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4566
- KEGG:   ecs:ECs4082
- GeneTree:   EBGT00050000009890
- HOGENOM:   HBG675832
- OMA:   DQINNVH
- ProtClustDB:   PRK10470
- BioCyc:   ECOL83334:ECS4082-MONOMER
- GO:   GO:0005488
- InterPro:   IPR003489
- Gene3D:   G3DSA:3.30.160.100
- TIGRFAMs:   TIGR00741

Pfam domain/function: PF02482 Ribosomal_S30AE; SSF69754 Ribosomal_S30Ae/sigma54_mod

EC number: NA

Molecular weight: Translated: 10750; Mature: 10750

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVEKVTHTSDATLHVNGGE
CEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEECCCE
IHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVEKVTHTSDATLHVNGGE
CEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCEEEECCCE
IHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796