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The map label for this gene is agaS [H]

Identifier: 157162621

GI number: 157162621

Start: 3332901

End: 3334055

Strand: Direct

Name: agaS [H]

Synonym: EcHS_A3328

Alternate gene names: 157162621

Gene position: 3332901-3334055 (Clockwise)

Preceding gene: 157162620

Following gene: 157162622

Centisome position: 71.78

GC content: 56.54

Gene sequence:

>1155_bases
ATGCCAAAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGACTGAAGAAGAGATCCGCCATCAGCCTCGCGCATG
GATCCGTTCACTCACCAACATCGACGCGCTACGTTCCGCGCTCAATAACTTCCTTGAACCGTTACTGCGCAAAGAGAATC
TGCGGATCATCCTGACCGGAGCCGGAACCTCGGCATTTATCGGTGACATCATCGCGCCGTGGCTCGCCAGCCATACCGGT
AAAAACTTCAGCGCCGTACCGACCACCGATCTGGTCACTAATCCGATGGACTACCTGAACCCAGCCCATCCGCTGCTGTT
GATCTCCTTCGGTCGATCCGGCAACAGCCCGGAAAGCGTCGCAGCCGTGGAACTGGCAAATCAATTTGTACCGGAATGCT
ATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTG
ATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTT
CGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATT
TCAGCGAAGGTGTGTTTGGTTACGCACCGTGGAAACGGATCGTTTATCTCGGTAGCGGTGGCTTACAGGGCGCAGCACGC
GAGTCGGCGCTGAAAGTGCTGGAACTGACGGCGGGTAAACTGGCGGCCTTTTATGATTCTCCAACCGGATTCCGTCATGG
ACCAAAATCGCTGGTCGATGACGAAACGCTGGTGGTGGTATTTGTCTCCAGCCACCCTTACACCCGTCAGTATGATCTTG
ATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCT
GCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCA
GACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGG
TGCAAGGCGTAATCATTCATCCGTGGCAGGCATAA

Upstream 100 bases:

>100_bases
AGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTC
ATCAGACTAAGGATTGAGTT

Downstream 100 bases:

>100_bases
GAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTT
TAACATTCATAACGCCGAGA

Product: AgaS family sugar isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFLEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTG
KNFSAVPTTDLVTNPMDYLNPAHPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGVFGYAPWKRIVYLGSGGLQGAAR
ESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVA
AGPHIILPPSRHFIDVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA

Sequences:

>Translated_384_residues
MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFLEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTG
KNFSAVPTTDLVTNPMDYLNPAHPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGVFGYAPWKRIVYLGSGGLQGAAR
ESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVA
AGPHIILPPSRHFIDVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA
>Mature_383_residues
PKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFLEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGK
NFSAVPTTDLVTNPMDYLNPAHPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALLM
PAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGVFGYAPWKRIVYLGSGGLQGAARE
SALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAA
GPHIILPPSRHFIDVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA

Specific function: May be a tagatose-6-phosphate ketose/aldose isomerase [H]

COG id: COG2222

COG function: function code M; Predicted phosphosugar isomerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains [H]

Homologues:

Organism=Escherichia coli, GI1789525, Length=384, Percent_Identity=99.7395833333333, Blast_Score=794, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001347
- InterPro:   IPR014180 [H]

Pfam domain/function: PF01380 SIS [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41782; Mature: 41651

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFLEPLLRKENLRIILTG
CCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
AGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPAHPLLLISFGRSGNSPESV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHCCCCCCEEEEEECCCCCCCHHH
AAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALLMPAETHDRGFAMTSSITTMM
HHHHHHHHHCHHHEECCEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCEEHHHHHHHHH
ASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGVFGYAPWKRIVYLGSGGLQGAAR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEECCCCCCCHHH
ESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVFVSSHPYTRQYDLDLLAELR
HHHHHHHHHHHCHHEEEECCCCCCCCCCHHHCCCCEEEEEEECCCCCCEECCHHHHHHHH
RDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFIDVEQAFCFLMYAQTFALMQSLHMGNT
CCCCEEEEEEEEECCCCEEEECCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCC
PDTPSASGTVNRVVQGVIIHPWQA
CCCCCCCCHHHHHHCCCEECCCCC
>Mature Secondary Structure 
PKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFLEPLLRKENLRIILTG
CCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
AGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPAHPLLLISFGRSGNSPESV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHCCCCCCEEEEEECCCCCCCHHH
AAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALLMPAETHDRGFAMTSSITTMM
HHHHHHHHHCHHHEECCEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCEEHHHHHHHHH
ASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGVFGYAPWKRIVYLGSGGLQGAAR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEEEECCCCCCCHHH
ESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVFVSSHPYTRQYDLDLLAELR
HHHHHHHHHHHCHHEEEECCCCCCCCCCHHHCCCCEEEEEEECCCCCCEECCHHHHHHHH
RDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFIDVEQAFCFLMYAQTFALMQSLHMGNT
CCCCEEEEEEEEECCCCEEEECCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCC
PDTPSASGTVNRVVQGVIIHPWQA
CCCCCCCCHHHHHHCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8932697 [H]