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The map label for this gene is garD [H]

Identifier: 157162611

GI number: 157162611

Start: 3323902

End: 3325473

Strand: Direct

Name: garD [H]

Synonym: EcHS_A3318

Alternate gene names: 157162611

Gene position: 3323902-3325473 (Clockwise)

Preceding gene: 157162610

Following gene: 157162612

Centisome position: 71.58

GC content: 52.8

Gene sequence:

>1572_bases
ATGGCCAACATCGAAATCAGACAAGAAACGCCAACTGCGTTTTATATAAAAGTTCACGACACAGATAATGTGGCAATTAT
TGTTAATGATAATGGCCTGAAAGCAGGAACGCGTTTTCCGGATGGGCTGGAATTAATTGAACATATTCCCCAGGGGCATA
AAGTCGCATTGCTGGACATTCCGGCTAATGGTGAAATTATTCGTTATGGCGAAGTGATTGGTTACGCCGTGCGTGCAATC
TCACGCGGAAGCTGGATCGACGAATCAATGGTTGTACTACCGGAAGCGCCGCCGTTACACACGCTGCCACTGGCAACCAA
AGTCCCGGAACCCTTACCGCCGCTGGAAGGATACACCTTTGAGGGCTATCGCAATGCCGATGGCAGCGTGGGCACCAAAA
ACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTG
CTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGC
GGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGG
GTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATT
GTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACA
AAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCGGTGGCAGCGATGCGT
TTTCTGGTGTAACGGCAAACCCGGCGGTTGGCTATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCA
GAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGGA
GGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAG
GCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCTAAATCGGGTAAAAGCGCAATTGTTGAAGTGCTGTCG
CCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGT
GGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAA
TGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAA
ACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCA
ATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGA

Upstream 100 bases:

>100_bases
TTTAAACTCCTGACATTCTTATTTCACCCAATGAAGTCATTTATTTTTAAATGAGACCAGGTCCTCATTTTAATAACCCC
TGGCTGGAGAATATTGCACA

Downstream 100 bases:

>100_bases
TTTCTTTTGGAACGTCCCTCGCAAAATATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAT
GCATTTGCATTACCTTAGTT

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAI
SRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASI
VSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFS
EVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLS
PGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEE
TIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT

Sequences:

>Translated_523_residues
MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAI
SRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASI
VSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFS
EVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLS
PGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEE
TIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT
>Mature_522_residues
ANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIS
RGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLL
PKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASIV
SLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSE
VTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSP
GQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEET
IEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=523, Percent_Identity=99.8087954110899, Blast_Score=1072, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=512, Percent_Identity=31.8359375, Blast_Score=214, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 56392; Mature: 56261

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDI
CCCCEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
PANGEIIRYGEVIGYAVRAISRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTF
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHCCCCCCCCCCCEEE
EGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLLPKYPNVDGVVGLNHLYGCG
CCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHCCC
VAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASI
EEECCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCHHCCCCCCCEEEECCCCEE
VSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTAN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCC
PAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEE
CCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHH
TIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
ANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDI
CCCEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEE
PANGEIIRYGEVIGYAVRAISRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTF
CCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHCCCCCCCCCCCEEE
EGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLLPKYPNVDGVVGLNHLYGCG
CCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHCCC
VAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASI
EEECCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCHHCCCCCCCEEEECCCCEE
VSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTAN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCC
PAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
TDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCCC
VCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEE
CCCHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCHH
TIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]