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The map label for this gene is ygiC

Identifier: 157162513

GI number: 157162513

Start: 3225252

End: 3226412

Strand: Direct

Name: ygiC

Synonym: EcHS_A3215

Alternate gene names: 157162513

Gene position: 3225252-3226412 (Clockwise)

Preceding gene: 157162512

Following gene: 157162515

Centisome position: 69.46

GC content: 50.39

Gene sequence:

>1161_bases
ATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTA
CGGCGAGCCGTACTGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCG
AACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAA
CACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCTCGTCTTGATCTGGCGTGGGATGG
CACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCT
GGCTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGAT
CGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGG
AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAG
GTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTTATTTCCAACCTGTTCAAGCTGTATCCGTGGGAGTTTATGCTG
CGTGAGATGTTCTCCACCAAGCTGGAGGATGCAGGCGTACGCTGGCTGGAACCGGCGTGGAAGAGCATTATCTCCAACAA
GGCACTTCTACCGCTGTTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATC
CGCAAATGGAAAAATATGTGGTTAAGCCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACC
ATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGAATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAG
CTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCC
AGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAA

Upstream 100 bases:

>100_bases
CCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGT
TCAATGGGTGGCTGATACCG

Downstream 100 bases:

>100_bases
GCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCT
ATACCCTCAACCGGAATGGT

Product: glutathionylspermidine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPK
HTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKT
IEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE

Sequences:

>Translated_386_residues
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPK
HTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKT
IEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE
>Mature_386_residues
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPK
HTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKT
IEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli yjfC

Homologues:

Organism=Escherichia coli, GI1789416, Length=386, Percent_Identity=100, Blast_Score=804, Evalue=0.0,
Organism=Escherichia coli, GI1790629, Length=387, Percent_Identity=50.6459948320413, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789361, Length=391, Percent_Identity=27.3657289002558, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGIC_ECO57 (P0ADT7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85964
- PIR:   F91119
- RefSeq:   NP_289618.1
- RefSeq:   NP_311953.1
- ProteinModelPortal:   P0ADT7
- SMR:   P0ADT7
- EnsemblBacteria:   EBESCT00000025596
- EnsemblBacteria:   EBESCT00000060056
- GeneID:   916245
- GeneID:   961964
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4395
- KEGG:   ecs:ECs3926
- GeneTree:   EBGT00050000009387
- HOGENOM:   HBG320655
- OMA:   ALLPMLW
- ProtClustDB:   CLSK880562
- BioCyc:   ECOL83334:ECS3926-MONOMER
- InterPro:   IPR005494
- InterPro:   IPR016185

Pfam domain/function: PF03738 GSP_synth; SSF52440 PreATP-grasp-like

EC number: NA

Molecular weight: Translated: 45026; Mature: 45026

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCL
CCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCEECCCCCC
PTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLIDRFVELREQYGFQLLHLTCCR
HHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYV
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHH
VKPIFSREGANVSIIENGKTIEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQP
HHHHHCCCCCEEEEEECCCEEECCCCCCCCCCEEHHHCCCCCCCCCCEEEEEEEEECCCC
AGIGIREDRALITQDMSRFYPHIFVE
CCCCCCCCHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCL
CCCCCCCCCCCHHHHHHHCCCCEEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCEECCCCCC
PTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLIDRFVELREQYGFQLLHLTCCR
HHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYV
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHH
VKPIFSREGANVSIIENGKTIEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQP
HHHHHCCCCCEEEEEECCCEEECCCCCCCCCCEEHHHCCCCCCCCCCEEEEEEEEECCCC
AGIGIREDRALITQDMSRFYPHIFVE
CCCCCCCCHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796