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The map label for this gene is mdaB

Identifier: 157162504

GI number: 157162504

Start: 3217361

End: 3217942

Strand: Direct

Name: mdaB

Synonym: EcHS_A3206

Alternate gene names: 157162504

Gene position: 3217361-3217942 (Clockwise)

Preceding gene: 157162502

Following gene: 157162505

Centisome position: 69.29

GC content: 50.52

Gene sequence:

>582_bases
ATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACTGAACGACACCCTGACCGAAGT
CGCGGATGGCACACTGCGCGACCTCGGGCATGATGTTCGCATCGTTCGCGCCGACAGCGACTACGATGTCAAAGCGGAAG
TACAAAACTTTCTCTGGGCTGATGTGGTGATCTGGCAGATGCCAGGCTGGTGGATGGGCGCGCCGTGGACAGTGAAAAAA
TACATTGATGATGTATTCACCGAAGGTCACGGGACGCTGTATGCCAGCGATGGTCGTACCCGCAAAGATCCGTCGAAAAA
ATACGGTTCCGGCGGCCTGGTACAGGGCAAAAAATATATGCTTTCTCTGACCTGGAACGCACCAATGGAAGCCTTCACCG
AAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATG
GAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGTTCCCCGCTATACTGAAGAATATCGCAAGCA
TCTTGTGGAAATTTTTGGTTAA

Upstream 100 bases:

>100_bases
TTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACG
TCTCACTTTGAGGTTAAAAA

Downstream 100 bases:

>100_bases
CTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCG
TCAGGCGGTATTGGATCAGT

Product: modulator of drug activity B

Products: NADP; a reduced quinone; reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKK
YIDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG

Sequences:

>Translated_193_residues
MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKK
YIDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG
>Mature_192_residues
SNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKY
IDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGME
PLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG

Specific function: Unknown

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae HI_0648 and S.pombe SPAC5H10.05c

Homologues:

Organism=Escherichia coli, GI1789406, Length=193, Percent_Identity=100, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDAB_ECO57 (P0AEY7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85962
- PIR:   F91117
- RefSeq:   NP_289602.1
- RefSeq:   NP_311937.1
- PDB:   2AMJ
- PDBsum:   2AMJ
- ProteinModelPortal:   P0AEY7
- SMR:   P0AEY7
- EnsemblBacteria:   EBESCT00000026756
- EnsemblBacteria:   EBESCT00000055949
- GeneID:   916264
- GeneID:   958510
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4379
- KEGG:   ecs:ECs3910
- GeneTree:   EBGT00050000010022
- HOGENOM:   HBG289856
- OMA:   PTFICND
- ProtClustDB:   CLSK880554
- BioCyc:   ECOL83334:ECS3910-MONOMER
- InterPro:   IPR003680

Pfam domain/function: PF02525 Flavodoxin_2

EC number: NA

Molecular weight: Translated: 21891; Mature: 21760

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWA
CCCEEEEECCHHHHCCCCCCHHHHHHHCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYM
HEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCCCEEECCEEE
LSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGMEPLPTFIANDVIKMPDVPRY
EEEEECCCHHHHHHHHHHEECCCCCCEEECHHHCCCCCCCCCCHHHHHCCCCCCCCCCCH
TEEYRKHLVEIFG
HHHHHHHHHHHCC
>Mature Secondary Structure 
SNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRADSDYDVKAEVQNFLWA
CCEEEEECCHHHHCCCCCCHHHHHHHCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
DVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRKDPSKKYGSGGLVQGKKYM
HEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHCCCCCEEECCEEE
LSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGMEPLPTFIANDVIKMPDVPRY
EEEEECCCHHHHHHHHHHEECCCCCCEEECHHHCCCCCCCCCCHHHHHCCCCCCCCCCCH
TEEYRKHLVEIFG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; a quinone; Acceptor; NADPH [C]

Specific reaction: NADPH + a quinone = NADP + a reduced quinone Acceptor + NADPH = reduced acceptor + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796