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The map label for this gene is yghU

Identifier: 157162469

GI number: 157162469

Start: 3183557

End: 3184423

Strand: Direct

Name: yghU

Synonym: EcHS_A3169

Alternate gene names: 157162469

Gene position: 3183557-3184423 (Clockwise)

Preceding gene: 157162466

Following gene: 157162479

Centisome position: 68.56

GC content: 53.4

Gene sequence:

>867_bases
ATGACAGACAATACTTATCAGCCCGCGAAAGTCTGGACGTGGGATAAATCCGCTGGCGGCGCGTTCGCCAATATCAATCG
CCCGGTTTCTGGTCCGACGCATGAAAAAACGCTGCCCGTTGGCAAACACCCATTGCAACTTTATTCGCTGGGAACGCCGA
ACGGTCAGAAAGTAACGATTATGCTTGAGGAGCTGCTGGCGCTGGGCGTTACTGGTGCAGAGTACGACGCCTGGCTGATT
CGTATTGGCGATGGCGATCAATTCTCCAGCGGCTTTGTCGAAGTGAACCCAAACTCGAAGATCCCGGCGCTGCGCGATCA
TACGCATAATCCGCCGATCCGCGTGTTTGAATCTGGTTCGATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGC
CGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGT
TTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCT
CGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGC
CGTGGTTTGGCAACGTGGTGTTAGGTGGTGTGTATGATGCCGCTGAGTTTCTTGATGCGGGCAGTTATAAGCATGTACAA
CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGA
GCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAA

Upstream 100 bases:

>100_bases
TCATCGCAGCGGCTGGCGCAAAGGGTTAAAAAATTCACATTCTGTACAGCAAGTGACCTGCTACACTGCTTCAACACTAC
CACTCAGAAGGCAACTCACT

Downstream 100 bases:

>100_bases
GGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGA
TGCGACGCTGCCGCGTCTTA

Product: putative glutathione S-transferase YghU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHPLQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLI
RIGDGDQFSSGFVEVNPNSKIPALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGVYDAAEFLDAGSYKHVQ
RWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNTEDKRQG

Sequences:

>Translated_288_residues
MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHPLQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLI
RIGDGDQFSSGFVEVNPNSKIPALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGVYDAAEFLDAGSYKHVQ
RWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNTEDKRQG
>Mature_287_residues
TDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHPLQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIR
IGDGDQFSSGFVEVNPNSKIPALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGGF
GHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGVYDAAEFLDAGSYKHVQR
WAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNTEDKRQG

Specific function: Unknown

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain

Homologues:

Organism=Escherichia coli, GI87082195, Length=288, Percent_Identity=100, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1788640, Length=214, Percent_Identity=41.1214953271028, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081789, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324100, Length=257, Percent_Identity=29.9610894941634, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571670, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864592, Length=201, Percent_Identity=27.8606965174129, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGHU_ECOLI (Q46845)

Other databases:

- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003544.1
- RefSeq:   NP_417463.4
- PDB:   3C8E
- PDBsum:   3C8E
- ProteinModelPortal:   Q46845
- SMR:   Q46845
- DIP:   DIP-12212N
- STRING:   Q46845
- EnsemblBacteria:   EBESCT00000001040
- EnsemblBacteria:   EBESCT00000017923
- GeneID:   947472
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5492
- KEGG:   eco:b2989
- EchoBASE:   EB2827
- EcoGene:   EG13005
- eggNOG:   COG0625
- GeneTree:   EBGT00050000009210
- HOGENOM:   HBG753188
- OMA:   HPFQLYS
- ProtClustDB:   PRK11752
- BioCyc:   EcoCyc:G7553-MONOMER
- Genevestigator:   Q46845
- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:1.20.1050.10
- Gene3D:   G3DSA:3.40.30.10

Pfam domain/function: PF00043 GST_C; PF02798 GST_N; SSF47616 GST_C_like; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 32392; Mature: 32261

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50405 GST_CTER; PS50404 GST_NTER

Important sites: BINDING 54-54 BINDING 87-87 BINDING 101-101 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHPLQLYSLGTPNGQKVTI
CCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
MLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIPALRDHTHNPPIRVFESGS
EHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCHHCCCCCCCEEEEECCC
ILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGGFGHFYHYAPVKIEYAINRFT
EEEEEEHHCCCCCCHHHHHHHHHHEEEEEECCCCCHHCCCHHHHEEECCEEEEEEHHHHH
MEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGVYDAAEFLDAGSYKHVQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHCCHHHHHHHHCCCCHHHHH
RWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNTEDKRQG
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHPLQLYSLGTPNGQKVTI
CCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
MLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIPALRDHTHNPPIRVFESGS
EHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCHHCCCCCCCEEEEECCC
ILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGGFGHFYHYAPVKIEYAINRFT
EEEEEEHHCCCCCCHHHHHHHHHHEEEEEECCCCCHHCCCHHHHEEECCEEEEEEHHHHH
MEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGNVVLGGVYDAAEFLDAGSYKHVQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHCCHHHHHHHHCCCCHHHHH
RWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDASDFETNTEDKRQG
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503