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The map label for this gene is bioF [H]

Identifier: 157162458

GI number: 157162458

Start: 3171108

End: 3172280

Strand: Direct

Name: bioF [H]

Synonym: EcHS_A3158

Alternate gene names: 157162458

Gene position: 3171108-3172280 (Clockwise)

Preceding gene: 157162457

Following gene: 157162466

Centisome position: 68.29

GC content: 52.34

Gene sequence:

>1173_bases
ATGGGGCTATACGATAAATATGCGCGCCTTGCTGGCGAGCGACTGCAATTTAGTGACAACGGGTTAACACCGTTTGGCAC
CTGTATTGATGAAGTGTATTCCGCCACCGAAGGCCGGATCGGCAATAAAAAAGTGATTCTGGCCGGGACCAATAATTACC
TCGGTTTAACCTTTAATCATGATGCCATCGCCGAAGGTCAGGCAGCGTTAGCGGCCCAAGGCACCGGCACCACCGGTTCT
CGTATGGCAAACGGCAGTTATGCGCCGCACCTGGCGTTAGAGAAAGAGATTGCCGAGTTTTTCAATCGCCCTACCGCTAT
CGTTTTTTCAACCGGTTATACCGCTAATTTAGGCGTCATCAGCGCACTTGCCGACCATAACGCCGTGGTGTTACTGGATG
CCGACAGCCACGCCAGTATTTATGACGCCTGTTCTCTCGGTGGCGCGGAAATTATTCGTTTCCGCCATAACGATGCTAAA
GATCTGGAACGGCGCATGGTTCGCCTCGGTGAACGGGCGAAAGAGGCGATTATCATCGTTGAGGGCATCTACAGTATGCT
CGGTGACGTTGCGCCGCTGGCGGAAATTGTTGATATCAAACGTCGTCTCGGTGGTTATTTGATTGTCGATGAAGCGCATT
CGTTTGGCGTGTTAGGCGCAACGGGGCGCGGACTGGCTGAAGCTGTCGGCGTTGAAGATGATGTGGATATTATCGTTGGT
ACTTTCAGTAAAAGTCTGGCCTCGATTGGCGGTTTTGCTGTCGGTTCGGAAGCGATGGAAGTGCTGCGCTACGGCAGCCG
CCCGTATATTTTTACCGCCTCTCCTTCTCCGTCCTGCATTGCGACCGTGCGCTCATCGTTAAGAACCATTGCCACACAAC
CAGAGCTGCGGCAAAAGCTGATGGATAACGCCAACCATCTGTATGACGGTCTGCAAAAACTGGGTTATGAGTTAAGTTCA
CATATTAGCCCGGTAGTGCCAGTAATAATCGGTTCGAAAGAGGAAGGTTTACGCATCTGGCGCGAGCTTATCTCGCTGGG
CGTGTATGTGAATCTTATCCTGCCGCCTGCCGCGCCTGCAGGCATCACGCTGCTGCGCTGTAGCGTAAATGCTGCCCACA
GTCGTGAGCAAATCGATGCCATCATTCAGGCATTTGCTACTCTGAAGCAGTAA

Upstream 100 bases:

>100_bases
AAGACAGATTCGATATTTCCATTCCTATTAATATTCTGCTGGATGTCAAAACCCCTGCGCAATTAATGGAAACTCTACTT
CCATGGCTGGAGAATAAATA

Downstream 100 bases:

>100_bases
TGTGAGCCCGCCGTGGTGTTTGCGCCACGGCGGGTTTAATGTTGTCTGTCATACCAATACACCAGGCTCAACGCCACCAA
CACAAACGCTACCGGTAAAC

Product: putative serine palmitoyltransferase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKKVILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGS
RMANGSYAPHLALEKEIAEFFNRPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVG
TFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSS
HISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ

Sequences:

>Translated_390_residues
MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKKVILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGS
RMANGSYAPHLALEKEIAEFFNRPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVG
TFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSS
HISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ
>Mature_389_residues
GLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKKVILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSR
MANGSYAPHLALEKEIAEFFNRPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAKD
LERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGT
FSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSH
ISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI284448556, Length=364, Percent_Identity=32.1428571428571, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI7657118, Length=337, Percent_Identity=33.2344213649852, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4758668, Length=359, Percent_Identity=32.033426183844, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI119220554, Length=361, Percent_Identity=31.8559556786704, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI83977442, Length=354, Percent_Identity=28.2485875706215, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI83977440, Length=354, Percent_Identity=28.2485875706215, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI83977444, Length=354, Percent_Identity=28.2485875706215, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI5454084, Length=323, Percent_Identity=30.3405572755418, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4502025, Length=362, Percent_Identity=27.9005524861878, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI40316939, Length=362, Percent_Identity=27.9005524861878, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790046, Length=347, Percent_Identity=33.7175792507205, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1786993, Length=348, Percent_Identity=32.183908045977, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71994529, Length=351, Percent_Identity=33.6182336182336, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI32566772, Length=372, Percent_Identity=33.0645161290323, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17560914, Length=362, Percent_Identity=30.939226519337, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560912, Length=362, Percent_Identity=30.939226519337, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71982617, Length=292, Percent_Identity=30.8219178082192, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982625, Length=292, Percent_Identity=30.8219178082192, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320267, Length=355, Percent_Identity=31.2676056338028, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320438, Length=367, Percent_Identity=27.7929155313352, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323954, Length=281, Percent_Identity=30.6049822064057, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24662918, Length=346, Percent_Identity=33.8150289017341, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI17136286, Length=399, Percent_Identity=31.077694235589, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137420, Length=386, Percent_Identity=27.720207253886, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24653280, Length=277, Percent_Identity=30.3249097472924, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24653276, Length=277, Percent_Identity=30.3249097472924, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24653278, Length=277, Percent_Identity=30.3249097472924, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 41725; Mature: 41594

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKKVILAGTNNYLGLTFNH
CCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECC
DAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFNRPTAIVFSTGYTANLGVI
CHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
SALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIV
HHHCCCCEEEEEECCCCCCHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHEEEEH
EGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCHHHHCCCCCCCCEEEE
TFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKL
HHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHH
MDNANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPA
HHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCEEEEEEECCCCCC
GITLLRCSVNAAHSREQIDAIIQAFATLKQ
CEEEEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKKVILAGTNNYLGLTFNH
CCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECC
DAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFNRPTAIVFSTGYTANLGVI
CHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
SALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIV
HHHCCCCEEEEEECCCCCCHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHHHHHEEEEH
EGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCHHHHCCCCCCCCEEEE
TFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKL
HHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHH
MDNANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISLGVYVNLILPPAAPA
HHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCEEEEEEECCCCCC
GITLLRCSVNAAHSREQIDAIIQAFATLKQ
CEEEEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2110099 [H]