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The map label for this gene is glcD [H]

Identifier: 157162452

GI number: 157162452

Start: 3164156

End: 3165646

Strand: Reverse

Name: glcD [H]

Synonym: EcHS_A3152

Alternate gene names: 157162452

Gene position: 3165646-3164156 (Counterclockwise)

Preceding gene: 157162454

Following gene: 157162451

Centisome position: 68.17

GC content: 56.81

Gene sequence:

>1491_bases
TTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGAGCATGTCCC
TGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCACGCGTCCAT
TACTGGTTGTTCTGCCTAAGCAAATGGAACAGGTGACAGCGATTCTGGCTGTCTGCCATCGCCTGCGTGTACCGGTGGTG
ACCCGTGGTGCAGGCACCGGGCTTTCTGGTGGCGCGCTGCCGCTGGAAAAAGGTGTGTTGTTGGTGATGGCGCGCTTTAA
AGAGATCCTCGACATTAACCCCGTTGGTCGCCGCGCGCGCGTGCAGCCAGGCGTGCGTAACCTGGCGATCTCCCAGGCCG
TTGCACCGCATAATCTCTACTACGCACCGGACCCTTCCTCACAAATCGCCTGTTCCATTGGCGGCAATGTGGCTGAAAAT
GCCGGCGGCGTCCACTGCCTGAAATATGGTCTGACCGTACATAACCTGCTGAAAATTGAAGTGCAAACGCTGGACGGCGA
GGCACTGACGCTGGGATCGGACGCGCTGGATTCACCTGGTTTTGACCTGCTGGCGCTGTTCACCGGATCGGAAGGTATGC
TCGGCGTGACCACCGAAGTGACGGTAAAACTGCTGCCGAAGCCGCCCGTGGCGCGGGTTCTGTTAGCCAGCTTTGACTCG
GTAGAAAAAGCCGGACTTGCGGTTGGTGACATCATCGCCAATGGCATTATCCCCGGCGGGCTGGAGATGATGGATAACCT
GTCGATCCGCGCGGCGGAAGATTTTATTCATGCCGGTTATCCCGTCGACGCCGAAGCGATTTTGTTATGCGAGCTGGACG
GCGTGGAGTCTGACGTACAGGAAGACTGCGAGCGGGTTAACGACATCTTGTTGAAAGCGGGCGCGACTGACGTCCGTCTG
GCACAGGACGAAGCAGAGCGCGTACGTTTCTGGGCCGGTCGCAAAAATGCGTTCCCGGCGGTAGGACGTATCTCCCCGGA
TTACTACTGCATGGATGGCACCATCCCGCGTCGCGCCCTGCCTGGCGTACTGGAAGGCATTGCCCGTTTATCGCAGCAAT
ATGATTTACGTGTTGCCAACGTCTTTCATGCCGGAGATGGCAACATGCACCCGTTAATCCTTTTCGATGCCAACGAACCC
GGTGAATTTGCCCGCGCGGAAGAGCTGGGCGGGAAGATCCTCGAACTCTGCGTTGAAGTTGGCGGCAGCATCAGTGGCGA
ACATGGCATCGGGCGAGAAAAAATCAATCAAATGTGCGCCCAGTTCAACAGCGATGAAATCACGACCTTCCATGCGGTCA
AGGCGGCGTTTGACCCCGATGGTTTGCTGAACCCTGGGAAAAACATTCCCACGCTACACCGCTGTGCTGAATTTGGTGCC
ATGCATGTGCATCACGGTCATTTACCTTTCCCTGAACTGGAGCGTTTCTGA

Upstream 100 bases:

>100_bases
AGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACACAACAAAGC
GAAGCCTACTCATGAGCATC

Downstream 100 bases:

>100_bases
TGCTACGCGAGTGTGATTACAGCCAGGCGCTGCTGGAGCAGGTGAATCAGGCGATTAGCGATAAAACGCCGCTGGTGATT
CAGGGCAGCAATAGCAAAGC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF

Sequences:

>Translated_496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF
>Mature_496_residues
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVV
TRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAEN
AGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRL
AQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEP
GEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
MHVHHGHLPFPELERF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=30.5882352941176, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=30.1339285714286, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI119964728, Length=469, Percent_Identity=28.7846481876333, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1789351, Length=496, Percent_Identity=99.7983870967742, Blast_Score=1003, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=24.0556660039761, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=28.3062645011601, Blast_Score=178, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=431, Percent_Identity=26.6821345707657, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=27.2980501392758, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17534361, Length=471, Percent_Identity=28.2377919320594, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17556096, Length=453, Percent_Identity=21.1920529801325, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320023, Length=454, Percent_Identity=29.295154185022, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320764, Length=475, Percent_Identity=27.3684210526316, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320027, Length=436, Percent_Identity=25.9174311926606, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18921117, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639277, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639275, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653753, Length=474, Percent_Identity=21.5189873417722, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 53499; Mature: 53499

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPK
CCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEECCH
QMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRAR
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCC
VQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIE
CCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEEE
VQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
EEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQ
HHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCHHHH
EDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRAL
HHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHH
PGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELCVEV
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
MHVHHGHLPFPELERF
EEEECCCCCCHHHHCC
>Mature Secondary Structure
MYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPK
CCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEECCH
QMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRAR
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCCC
VQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIE
CCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEEEEE
VQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASFDS
EEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
VEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQ
HHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCHHHH
EDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRAL
HHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHH
PGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELCVEV
HHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
MHVHHGHLPFPELERF
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]