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The map label for this gene is gspD2 [H]

Identifier: 157162438

GI number: 157162438

Start: 3146921

End: 3148981

Strand: Reverse

Name: gspD2 [H]

Synonym: EcHS_A3138

Alternate gene names: 157162438

Gene position: 3148981-3146921 (Counterclockwise)

Preceding gene: 157162439

Following gene: 157162437

Centisome position: 67.81

GC content: 54.73

Gene sequence:

>2061_bases
GTGTTTTGGCGTGATATTACGTTGTCGGTCTGGCGTAAGAAGACAACTGGCCTCAAAACAAAAAAGCGTTTACTGCCGCT
GGTGCTGGCAGCGGCATTATGCAGTTCACCGGTCTGGGCGGAAGAAGCCACTTTCACCGCTAATTTTAAAGATACCGACC
TGAAATCGTTCATCGAAACCGTCGGCGCTAACCTTAATAAAACCATCATTATGGGGCCGGGCGTACAGGGGAAAGTGAGT
ATTCGCACCATGACCCCGCTCAATGAACGCCAGTATTACCAGCTATTCCTTAACCTGCTGGAAGCACAGGGGTATGCCGT
CGTACCGATGGAAAACGACGTGCTGAAGGTGGTGAAATCCAGCGCTGCGAAAGTCGAGCCGCTGCCGCTGGTCGGTGAAG
GCAGCGACAACTACGCGGGCGATGAAATGGTCACCAAAGTAGTGCCGGTACGTAATGTGTCAGTACGCGAGCTGGCACCG
ATTCTGCGCCAGATGATCGACAGCGCAGGCTCAGGCAACGTTGTTAATTACGATCCCTCCAACGTGATTATGCTCACCGG
ACGCGCCTCCGTCGTGGAGCGGCTGACGGAAGTGATCCAGAGTGTGGATCACGCGGGTAATCGCACTGAAGAGGTGATCC
CGCTGGATAACGCCTCGGCTTCGGAAATCGCCCGCGTGCTGGAAAGCCTGACGAAAAACAGCGGCGAGAACCAGCCTGCG
ACGCTGAAATCTCAAATTGTCGCCGATGAACGCACCAACAGTGTGATTGTCAGTGGTGACCCCGCCACGCGGGACAAAAT
GCGCCGTCTGATCCGTCGCTTGGACTCAGAAATGGAGCGCAGCGGCAACAGCCAGGTTTTCTATCTCAAATACAGCAAAG
CCGAAGATCTGGTCGATGTACTGAAGCAGGTCAGCGGCACGCTCACGGCGGCTAAAGAAGAGGCGGAAGGCACGGTCGGT
AGCGGGCGTGAGATTGTCTCCATCGCCGCCAGCAAACACAGTAATGCCCTGATTGTTACTGCGCCGCAGGACATCATGCA
ATCGCTGCAAAGCGTGATTGAACAACTGGATATTCGCCGTGCTCAGGTGCATGTCGAGGCGTTGATCGTGGAAGTTGCCG
AAGGTAGCAATATCAATTTCGGCGTGCAGTGGGCGTCGAAAGATGCCGGATTAATGCAGTTTGCTAATGGTACGCAGATC
CCTATCGGTACGCTGGGCGCAGCCATTTCTCAGGCGAAACCGCAGAAAGGCTCGACGGTGATCAGTGAAAACGGCGCTAC
CACCATTAATCCGGATACCAACGGCGATCTCTCCACGCTCGCCCAGCTTCTTTCCGGCTTTAGCGGTACGGCGGTTGGTG
TGGTGAAAGGCGACTGGATGGCGCTGGTGCAGGCGGTTAAAAACGACTCCAGCTCTAACGTGCTCTCCACGCCGAGCATC
ACCACGCTGGACAACCAGGAAGCCTTCTTCATGGTGGGCCAGGACGTTCCGGTATTAACCGGCTCTACCGTTGGCTCCAA
TAACAGCAATCCTTTCAATACAGTAGAAAGGAAAAAAGTCGGCATCATGCTGAAAGTCACGCCGCAGATTAACGAAGGTA
ACGCGGTACAGATGGTGATTGAGCAGGAAGTCTCGAAGGTGGAAGGACAGACCAGCCTCGACGTGGTGTTTGGTGAGCGC
AAACTGAAAACCACCGTGCTGGCTAACGATGGTGAGCTGATTGTGCTTGGTGGTCTGATGGACGACCAGGCGGGAGAAAG
CGTGGCGAAAGTGCCGCTGCTTGGCGATATCCCATTAATTGGTAACCTGTTTAAATCGACGGCGGATAAAAAAGAAAAAC
GTAACCTGATGGTGTTTATCCGTCCGACCATTCTGCGTGACGGTATGGCGGCAGACGGCGTGTCGCAGCGCAAATATAAC
TATATGCGCGCCGAACAGATCTATCGCGATGAGCAAGGCTTAAGCCTGATGCCGCACACCGCACAGCCGGTACTGCCAGC
GCAAAACCAGGCCTTACCGCCGGAAGTTCGCGCGTTCCTCAATGCCGGGAGAACGCGTTAA

Upstream 100 bases:

>100_bases
CAATGGATTCCATTCAACTTACGGTTTTACGCAAGGGTGCGCGCCACGACATTTCCATCGCGCTGCGCTAACCGCATTTA
ATCCAGGAGAATCATTCATC

Downstream 100 bases:

>100_bases
TGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGCA
TGGTGCGAAGCGTCGCTGGA

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQSVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREIVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQI
PIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNAGRTR

Sequences:

>Translated_686_residues
MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQSVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREIVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQI
PIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNAGRTR
>Mature_686_residues
MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVS
IRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQSVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVG
SGREIVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQI
PIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGER
KLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYN
YMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNAGRTR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=654, Percent_Identity=44.3425076452599, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789793, Length=307, Percent_Identity=24.7557003257329, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 74134; Mature: 74134

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIET
CCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
VGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKS
HCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
SAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMIDSAGSGNVVNYDPS
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
NVIMLTGRASVVERLTEVIQSVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
LKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRR
HHHHHCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQIPIGTLGAAISQAKPQKGSTV
HHHEEEHHEEEECCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCEE
ISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
EECCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCE
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVI
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCEEEHHH
EQEVSKVEGQTSLDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLI
HHHHHHHCCCCEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHH
GNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHT
HHHHHHHHCHHHCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AQPVLPAQNQALPPEVRAFLNAGRTR
CCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEATFTANFKDTDLKSFIET
CCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
VGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAVVPMENDVLKVVKS
HCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
SAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMIDSAGSGNVVNYDPS
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
NVIMLTGRASVVERLTEVIQSVDHAGNRTEEVIPLDNASASEIARVLESLTKNSGENQPA
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
TLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHH
LKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRR
HHHHHCHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQIPIGTLGAAISQAKPQKGSTV
HHHEEEHHEEEECCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCEE
ISENGATTINPDTNGDLSTLAQLLSGFSGTAVGVVKGDWMALVQAVKNDSSSNVLSTPSI
EECCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEECCCCE
TTLDNQEAFFMVGQDVPVLTGSTVGSNNSNPFNTVERKKVGIMLKVTPQINEGNAVQMVI
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCEEEHHH
EQEVSKVEGQTSLDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKVPLLGDIPLI
HHHHHHHCCCCEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCEEECCCCCCCCHH
GNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHT
HHHHHHHHCHHHCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AQPVLPAQNQALPPEVRAFLNAGRTR
CCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]