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The map label for this gene is pgk [H]

Identifier: 157162385

GI number: 157162385

Start: 3098970

End: 3100133

Strand: Reverse

Name: pgk [H]

Synonym: EcHS_A3084

Alternate gene names: 157162385

Gene position: 3100133-3098970 (Counterclockwise)

Preceding gene: 157162386

Following gene: 157162384

Centisome position: 66.76

GC content: 52.32

Gene sequence:

>1164_bases
ATGTCTGTAATTAAGATGACCGATCTGGATCTTGCTGGGAAACGTGTATTTATCCGTGCGGATCTGAACGTACCAGTAAA
AGACGGGAAAGTAACCAGCGACGCGCGTATCCGTGCTTCTCTGCCGACCATCGAACTGGCCCTGAAACAAGGCGCAAAAG
TGATGGTAACTTCCCACCTGGGTCGTCCTACCGAAGGCGAGTACAACGAAGAATTCTCTCTGCTGCCGGTTGTTAACTAC
CTGAAAGACAAACTGTCTAACCCGGTTCGTCTGGTTAAAGATTACCTCGACGGCGTTGACGTTGCTGAAGGTGAACTGGT
TGTTCTGGAAAACGTTCGCTTCAACAAAGGCGAGAAGAAAGACGACGAAACCCTGTCCAAAAAATACGCTGCACTGTGTG
ACGTGTTCGTAATGGACGCATTCGGTACTGCTCACCGCGCGCAGGCTTCTACTCACGGTATCGGTAAATTCGCTGACGTT
GCGTGCGCAGGCCCGCTGCTGGCAGCTGAACTGGACGCGCTGGGTAAAGCACTGAAAGAACCTGCTCGCCCGATGGTGGC
TATCGTTGGTGGTTCTAAAGTATCTACCAAACTGACCGTTCTGGACTCCCTGTCTAAAATCGCTGACCAGCTGATTGTTG
GTGGTGGTATCGCTAACACCTTTATCGCGGCACAAGGCCACGATGTGGGTAAATCCCTGTACGAAGCTGACCTGGTTGAC
GAAGCTAAACGTCTGCTGAGCACCTGCAACATCCCGGTTCCGTCTGATGTTCGCGTAGCAACCGAGTTCTCTGAAACTGC
ACCGGCTACCCTGAAATCTGTTAACGATGTGAAAGCTGACGAGCAGATCCTGGATATCGGTGATGCTTCCGCTCAGGAAC
TGGCTGAAATCCTGAAGAATGCGAAAACCATTCTGTGGAACGGTCCGGTTGGCGTGTTCGAATTCCCGAACTTCCGCAAA
GGTACTGAAATCGTGGCTAACGCTATCGCAGACAGCGAAGCGTTCTCCATCGCTGGCGGCGGCGACACTCTGGCAGCAAT
CGACCTGTTCGGCATTGCTGACAAAATCTCCTACATCTCCACTGGCGGCGGCGCATTCCTCGAATTCGTGGAAGGTAAAG
TACTGCCTGCAGTAGCGATGCTCGAAGAGCGCGCTAAGAAGTAA

Upstream 100 bases:

>100_bases
AACCGAATGCTCGACACGACGTTAGCTATGGCTACTGTTGCTTTCAGGTAAGACGCAAGCAGCGTCTGCAAAACTTTTAG
AATCAACGAGAGGATTCACC

Downstream 100 bases:

>100_bases
AAAATCACAGGGCAGGGAAACCTGCCCTTGTTTCAGCGCGCTTTTAGAGCACGCAATTTTTCATACTCTAAATAATTCGA
GTTGCAGGAAGGCGACAAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNY
LKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
EAKRLLSTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK

Sequences:

>Translated_387_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNY
LKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
EAKRLLSTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK
>Mature_386_residues
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYL
KDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVDE
AKRLLSTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKG
TEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=36.9829683698297, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=36.2530413625304, Blast_Score=234, Evalue=8e-62,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=99.7416020671835, Blast_Score=774, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=39.4673123486683, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319857, Length=416, Percent_Identity=39.6634615384615, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24581203, Length=416, Percent_Identity=39.9038461538462, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=39.0776699029126, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41105; Mature: 40973

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKE
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHH
EAKRLLSTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKN
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKVLPAVAMLEERAKK
CCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKE
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHH
EAKRLLSTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKN
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKVLPAVAMLEERAKK
CCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA