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The map label for this gene is argK

Identifier: 157162377

GI number: 157162377

Start: 3090498

End: 3091493

Strand: Direct

Name: argK

Synonym: EcHS_A3076

Alternate gene names: 157162377

Gene position: 3090498-3091493 (Clockwise)

Preceding gene: 157162376

Following gene: 157162378

Centisome position: 66.55

GC content: 53.92

Gene sequence:

>996_bases
ATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGAC
GCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACA
CCCTGCGACTGGGCGTTACCGGCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGA
GAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCG
CATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGC
GAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCG
GAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAA
AAAAGGGCTGATGGAAGTGGCTGATCTGATCGTTATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCC
GGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCA
CTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGTTTACA
ACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATG
AAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAG
CTCAGTGAATTTATCCAGACGCAATATTTTGATTAA

Upstream 100 bases:

>100_bases
TACGCCTTCCTGCAAGAGCGCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGCGCGACGTACT
GAATCTGATAAGCCAGCATC

Downstream 100 bases:

>100_bases
AGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAA
AACTTAATGCCTTAAGTAAA

Product: arginine/ornithine transport system ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIR
EGLKVAVIAVDPSSPVTGGSILGDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYESALHILRRKYDEWQPRVLTCSA
LEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQ
LSEFIQTQYFD

Sequences:

>Translated_331_residues
MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIR
EGLKVAVIAVDPSSPVTGGSILGDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYESALHILRRKYDEWQPRVLTCSA
LEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQ
LSEFIQTQYFD
>Mature_331_residues
MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIR
EGLKVAVIAVDPSSPVTGGSILGDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYESALHILRRKYDEWQPRVLTCSA
LEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQ
LSEFIQTQYFD

Specific function: Phosphorylates the AO and LAO periplasmic-binding proteins

COG id: COG1703

COG function: function code E; Putative periplasmic protein kinase ArgK and related GTPases of G3E family

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArgK family

Homologues:

Organism=Homo sapiens, GI26892295, Length=287, Percent_Identity=47.3867595818815, Blast_Score=262, Evalue=3e-70,
Organism=Escherichia coli, GI1789285, Length=331, Percent_Identity=100, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71994271, Length=297, Percent_Identity=41.0774410774411, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI71994275, Length=235, Percent_Identity=44.2553191489362, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARGK_ECOLI (P27254)

Other databases:

- EMBL:   X66836
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E65076
- RefSeq:   AP_003476.1
- RefSeq:   NP_417393.1
- PDB:   2P67
- PDBsum:   2P67
- ProteinModelPortal:   P27254
- SMR:   P27254
- STRING:   P27254
- PRIDE:   P27254
- EnsemblBacteria:   EBESCT00000003054
- EnsemblBacteria:   EBESCT00000016711
- GeneID:   947412
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2885
- KEGG:   eco:b2918
- EchoBASE:   EB1415
- EcoGene:   EG11445
- eggNOG:   COG1703
- GeneTree:   EBGT00050000011631
- HOGENOM:   HBG728764
- OMA:   GVMELAD
- ProtClustDB:   PRK09435
- BioCyc:   EcoCyc:EG11445-MONOMER
- BioCyc:   MetaCyc:EG11445-MONOMER
- Genevestigator:   P27254
- GO:   GO:0006810
- InterPro:   IPR005129
- InterPro:   IPR016121
- Gene3D:   G3DSA:1.10.287.130
- TIGRFAMs:   TIGR00750

Pfam domain/function: PF03308 ArgK

EC number: 2.7.-.- [C]

Molecular weight: Translated: 36704; Mature: 36704

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCGNTLRLGVT
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSILGDKTRMNDLARAEAAFIR
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
PVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQSETEVARMVDCFISLQIAGGG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEECCCC
DDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYESALHILRRKYDEWQPRVLTCSA
CHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHH
LEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYY
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCGNTLRLGVT
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSILGDKTRMNDLARAEAAFIR
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
PVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQSETEVARMVDCFISLQIAGGG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEECCCC
DDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYESALHILRRKYDEWQPRVLTCSA
CHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHH
LEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYY
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Arg [C]

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9733684; 9278503