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The map label for this gene is ubiH [H]

Identifier: 157162367

GI number: 157162367

Start: 3079851

End: 3081029

Strand: Reverse

Name: ubiH [H]

Synonym: EcHS_A3066

Alternate gene names: 157162367

Gene position: 3081029-3079851 (Counterclockwise)

Preceding gene: 157162368

Following gene: 157162366

Centisome position: 66.35

GC content: 60.22

Gene sequence:

>1179_bases
ATGAGCGTAATCATCGTCGGTGGCGGCATGGCGGGCGCGACGCTGGCGCTGGCTATTTCCCGGTTAAGTCACGGGGCGCT
GCCGGTACATTTGATTGAAGCGACTGCGCCAGAGTCACATGCTCATCCGGGCTTTGATGGACGAGCGATAGCGCTGGCGG
CGGGTACCTGTCAGCAACTGGCGCGCATCGGCGTCTGGCAATCTCTGGCGGATTGCGCAACTGCCATCACCACCGTGCAT
GTCAGCGATCGTGGTCACGCTGGATTTGTCACCCTCGCCGCAGAAGATTACCAACTGGCGGCGCTGGGACAGGTTGTCGA
ATTGCACAATGTCGGGCAACGGCTGTTTGCATTGCTGCGTAAAGCACCTGGCGTAACGCTGCATTGCCCTGATCGCGTGG
CTAACGTTGCCCGTACTCAGAGTCACGTTGAAGTGACGCTGGAGAGTGGCGAGACGCTGACGGGCCGCGTGCTGGTAGCC
GCTGATGGCACCCATTCAGCGTTAGCCACCGCGTGCGGCGTTGACTGGCAGCAGGAGCCTTACGAACAACTGGCAGTGAT
TGCCAACGTTGCTACTTCTGTTGCGCACGAAGGCCGCGCTTTTGAACGCTTCACGCAACATGGCCCGCTGGCGATGTTGC
CGATGTCTGACGGACGCTGTTCGCTGGTCTGGTGTCATCCACTGGAACGGCGCGAAGAGGTGCTGTCGTGGAGTGACGAG
AAGTTTTGCCGTGAGCTCCAGTCGGCCTTTGGCTGGCGACTTGGAAAAATTACCCACGCCGGTAAACGCAGTGCTTATCC
GCTGGCGTTAACCCGCGCCGCCAGAGCTATTACCCATCGTACCGTGCTGGTGGGCAATGCGGCGCAAACCCTGCACCCGA
TTGCCGGGCAAGGGTTCAACCTCGGTATGCGAGATGTGATGAGTCTTGCGGAAACCCTGACTCAGGCGCAGGAGCGCGGA
GAAGACATGGGGGATTACGGCGTATTGTGCCGTTATCAGCAGCGTCGACAGAGCGATCGCGAAGCAACCATCGGCGTCAC
GGACAGCCTGGTACATCTTTTTGCCAACCGTTGGGCACCGCTGGTTGTCGGGCGCAACATCGGACTGATGACGATGGAAT
TATTCACCCCGGCACGCGATGTGCTGGCGCAGCGCACCCTCGGTTGGGTGGCGCGTTGA

Upstream 100 bases:

>100_bases
ACGACATTGTGATTACCGAAACCGGTAACGAAAACCTCACCGCCAGCGTGGTGAAAAAGCCGGAAGAAATCGAAGCGTTG
ATGGCTGCTGCGAGAAAGCA

Downstream 100 bases:

>100_bases
AGGCGGTTTTAAGGAATAAACAATGCAAAGTGTTGATGTAGCCATTGTTGGTGGCGGCATGGTGGGGCTGGCGGTTGCCT
GCGGCTTACAGGGGAGCGGC

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Sequences:

>Translated_392_residues
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITTVH
VSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERG
EDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
>Mature_391_residues
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHV
SDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAA
DGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDEK
FCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGE
DMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=386, Percent_Identity=29.0155440414508, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI32967307, Length=374, Percent_Identity=29.9465240641711, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=99.4897959183673, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=30.2798982188295, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786883, Length=372, Percent_Identity=31.989247311828, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=425, Percent_Identity=25.8823529411765, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=24.7240618101545, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19920760, Length=420, Percent_Identity=23.5714285714286, Blast_Score=121, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42292; Mature: 42161

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHHEECCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
EEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHH
ARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
HHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEP
HCCCCEEECCHHHHHHHHCCCEEEEEEECCCEEEEEEEEEECCCHHHHHHHHCCCCCCCH
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCHH
KFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARAITHRTVLVGNAAQTLHPIAGQGFN
HHHHHHHHHHCCCHHHEECCCCCCCCCHHHHHHHHHHHHHEEEECCCHHHHCCCCCCCCC
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAP
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCCCCEECCHHHHHHHHHHCCCCE
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
EEEECCCCEEEHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]