BacMap

Definition	Escherichia coli HS, complete genome.
Accession	NC_009800
Length	4,643,538

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The map label for this gene is ygfF [H]

Identifier: 157162362

GI number: 157162362

Start: 3072671

End: 3073414

Strand: Reverse

Name: ygfF [H]

Synonym: EcHS_A3061

Alternate gene names: 157162362

Gene position: 3073414-3072671 (Counterclockwise)

Preceding gene: 157162363

Following gene: 157162360

Centisome position: 66.19

GC content: 54.7

Gene sequence:

>744_bases
ATGGCTATAGCACTTGTGACCGGTGGCAGTCGCGGCATCGGGCGGGCAACTGCATTACTGTTGGCGAAAGAAGAGTATAC
GGTGGCGGTTAATTATCAACAAAACCTCCATGCGGCGCAGGAAGTGGTGAACTTAATCACGCAAGCCGGTGGCAAGGCAT
TCGTGCTCCAGGCGGATATCAGCGACGAAAATCAGGTCATCGCAATGTTTACAGCAATCGATCAGCACGATGAACCGCTA
GCAGCGCTGGTCAATAACGCCGGGATCTTGTTTACCCAGTGCACCGTTGAAAACCTTACCGCAGAGCGAATCAACCGAGT
ACTTTCCACCAACGTGACGGGATATTTTCTCTGCTGCCGCGAGGCGGTAAAACGCATGGCGCTTAAAAATGGTGGCAGTG
GCGGCGCTATCGTCAATGTCTCTTCGGTGGCCTCACGGTTGGGTTCGCCAGGGGAATATGTTGATTACGCGGCATCGAAA
GGGGCGATTGATACGTTAACCACCGGACTATCGCTGGAAGTCGCCGCGCAGGGGATCCGCGTTAACTGCGTGCGGCCAGG
GTTTATTTATACCGAAATGCACGCCAGCGGCGGCGAGCCTGGACGCGTCGATCGCGTTAAGTCGAACATCCCCATGCAGC
GTGGTGGACAGGCAGAAGAGGTCGCGCAGGCCATTGTCTGGCTACTAAGTGATAAAGCCTCTTACGTCACGGGAAGTTTT
ATCGATTTGGCGGGCGGGAAATAA

Upstream 100 bases:

>100_bases
TGAAAGGGCGAGGGGAAAAGCGTGCCAACATTGAAGATTGAGCCAGTTTGTTAGCAATCTCAAAGATACGTCAACGAATT
AATTTTTCTCGGAAAAACAA

Downstream 100 bases:

>100_bases
AACAGGGAAGTTGTCTGACCGGATGCAACAAGTATTGCATCCGGTACTTCATCGACTTAAAGCTTCTCGCCGTTGCTGGC
AATCACCTCTTTGTACCAGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVNLITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPL
AALVNNAGILFTQCTVENLTAERINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSF
IDLAGGK

Sequences:

>Translated_247_residues
MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVNLITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPL
AALVNNAGILFTQCTVENLTAERINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSF
IDLAGGK
>Mature_246_residues
AIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVNLITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLA
ALVNNAGILFTQCTVENLTAERINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASKG
AIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSFI
DLAGGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=37.1428571428571, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI15277342, Length=254, Percent_Identity=33.8582677165354, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=33.201581027668, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=31.7269076305221, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI7705925, Length=244, Percent_Identity=31.5573770491803, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI109715829, Length=247, Percent_Identity=27.9352226720648, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI59889578, Length=201, Percent_Identity=28.8557213930348, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI5031737, Length=258, Percent_Identity=31.0077519379845, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=28.2868525896414, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI10190704, Length=251, Percent_Identity=27.8884462151394, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI31542939, Length=199, Percent_Identity=32.6633165829146, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI66933014, Length=175, Percent_Identity=32, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=98.3805668016194, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1789378, Length=246, Percent_Identity=34.9593495934959, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=29.5081967213115, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=33.1967213114754, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI2367365, Length=238, Percent_Identity=32.7731092436975, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786812, Length=250, Percent_Identity=31.6, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=29.5719844357977, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787905, Length=243, Percent_Identity=30.4526748971193, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082160, Length=247, Percent_Identity=29.1497975708502, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=28.740157480315, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789208, Length=245, Percent_Identity=26.9387755102041, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787526, Length=250, Percent_Identity=27.2, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787891, Length=244, Percent_Identity=26.2295081967213, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=255, Percent_Identity=32.5490196078431, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25147288, Length=244, Percent_Identity=34.0163934426229, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=30.859375, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=33.8709677419355, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI115534694, Length=243, Percent_Identity=32.9218106995885, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17536651, Length=250, Percent_Identity=32, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=28.5185185185185, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536025, Length=254, Percent_Identity=31.496062992126, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=32.1033210332103, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=259, Percent_Identity=30.8880308880309, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=256, Percent_Identity=29.296875, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=205, Percent_Identity=33.1707317073171, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=30.3501945525292, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=257, Percent_Identity=31.9066147859922, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=259, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=31.1538461538462, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=266, Percent_Identity=27.4436090225564, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=260, Percent_Identity=29.2307692307692, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=30.7086614173228, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=262, Percent_Identity=27.8625954198473, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=268, Percent_Identity=27.6119402985075, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=29.3893129770992, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=267, Percent_Identity=31.0861423220974, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=213, Percent_Identity=27.2300469483568, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=262, Percent_Identity=30.5343511450382, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=28.968253968254, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=247, Percent_Identity=28.3400809716599, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=29.811320754717, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=27.7992277992278, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=263, Percent_Identity=30.4182509505703, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=240, Percent_Identity=25, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=244, Percent_Identity=31.1475409836066, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324126, Length=252, Percent_Identity=26.5873015873016, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=36.5758754863813, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=36.4705882352941, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=35.15625, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571526, Length=254, Percent_Identity=34.251968503937, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24639444, Length=246, Percent_Identity=34.5528455284553, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643142, Length=245, Percent_Identity=33.469387755102, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24644337, Length=214, Percent_Identity=32.7102803738318, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355319, Length=253, Percent_Identity=30.0395256916996, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17737361, Length=238, Percent_Identity=33.1932773109244, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116007236, Length=242, Percent_Identity=28.5123966942149, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641232, Length=237, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25993; Mature: 25862

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVNLITQAGGKAFVLQADI
CEEEEEECCCCCCCHHEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECC
SDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAERINRVLSTNVTGYFLCCR
CCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHH
EAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASKGAIDTLTTGLSLEVAAQGIR
HHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCHHHHHCCCCCHHHHHCCCEEEEEECCEE
VNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSF
EEEECCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
IDLAGGK
EECCCCC
>Mature Secondary Structure 
AIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVNLITQAGGKAFVLQADI
EEEEEECCCCCCCHHEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECC
SDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAERINRVLSTNVTGYFLCCR
CCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHH
EAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASKGAIDTLTTGLSLEVAAQGIR
HHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCHHHHHCCCCCHHHHHCCCEEEEEECCEE
VNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQAEEVAQAIVWLLSDKASYVTGSF
EEEECCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
IDLAGGK
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 7764507 [H]