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The map label for this gene is ygfS [H]

Identifier: 157162346

GI number: 157162346

Start: 3056038

End: 3056526

Strand: Reverse

Name: ygfS [H]

Synonym: EcHS_A3045

Alternate gene names: 157162346

Gene position: 3056526-3056038 (Counterclockwise)

Preceding gene: 157162347

Following gene: 157162337

Centisome position: 65.82

GC content: 55.83

Gene sequence:

>489_bases
ATGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTGGCCTGTGTGGTCGCTCACCC
TTCAGAACAGGAGTTGAATGCCGATGTCTTTTTACCCCGGCTGAAGGTACAGCGACTGGACAGCATCAGTGCGCCGGTGA
TGTGCCATCAGTGTGAAAACGCCCCTTGTGTTGGCGCTTGCCCCGTGGGGGCGCTGACGATGGGCGAGCAGGTGGTGCAG
GCCAATTCTGCCCGTTGTATTGGCTGTCAGAGCTGCGTCAGTGCGTGCCCGTTTGGGATGATCACCATTCAGTCATTGCC
GGGGGATACCCGGCAACAAATCGTGAAATGCGATCTCTGTGAACAGCGGGAAGAGGGGCCAGCCTGCGTTGAATCTTGCC
CAACACAGGCGCTGCAGTTGCTTACTGAAAGAGAACTCAGGCGAGTCCGCCAGCAGCGTATTGTTGCCAGCGGTGAGAAT
CCTCTCTGA

Upstream 100 bases:

>100_bases
GGGATGCAGTTCATGGCGCGGATCTGGTTGTCACTGCCATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTG
TTTGATACGAAGGCATCGTG

Downstream 100 bases:

>100_bases
AAGAAATCTCCGCGCTGAAAGGCGCGGAGAATCTATTAGATTGCCCAACCACCCGCGTAAAATGCGACCAGTGCGGCAGT
AATGATCACCGTACCAATAT

Product: 4Fe-4S binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL

Sequences:

>Translated_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL
>Mature_162_residues
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQ
ANSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
PL

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=99.3827160493827, Blast_Score=320, Evalue=2e-89,
Organism=Escherichia coli, GI1788811, Length=166, Percent_Identity=44.578313253012, Blast_Score=141, Evalue=2e-35,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=45.859872611465, Blast_Score=137, Evalue=2e-34,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=46.0122699386503, Blast_Score=134, Evalue=2e-33,
Organism=Escherichia coli, GI1789067, Length=167, Percent_Identity=40.1197604790419, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=36.6336633663366, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1789079, Length=184, Percent_Identity=36.4130434782609, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1787749, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=26.9938650306748, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI1790326, Length=172, Percent_Identity=27.906976744186, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI2367345, Length=138, Percent_Identity=31.8840579710145, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17484; Mature: 17484

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
9.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCHHCCHHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGENPL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSISAPVMCHQCEN
CCCEEEECCHHHCCCCCEEEEEEEECCCCHHCCCHHCCHHHHHHHHHCCCCCEEEECCCC
APCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMITIQSLPGDTRQQIVKCDLC
CCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
EQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGENPL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]