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The map label for this gene is lepA [H]

Identifier: 157162048

GI number: 157162048

Start: 2743661

End: 2745460

Strand: Reverse

Name: lepA [H]

Synonym: EcHS_A2724

Alternate gene names: 157162048

Gene position: 2745460-2743661 (Counterclockwise)

Preceding gene: 157162049

Following gene: 157162047

Centisome position: 59.12

GC content: 53.72

Gene sequence:

>1800_bases
ATGAAGAATATACGTAACTTTTCGATCATAGCTCACATTGACCACGGTAAATCGACGCTGTCTGACCGTATTATCCAGAT
CTGCGGTGGCCTGTCTGACCGTGAAATGGAGGCGCAGGTTCTCGATTCCATGGATCTTGAGCGTGAGCGTGGCATTACCA
TCAAAGCGCAAAGCGTGACGCTGGACTACAAAGCGTCTGACGGCGAAACCTATCAGCTTAACTTTATCGACACCCCGGGC
CACGTAGACTTCTCCTATGAAGTTTCCCGTTCGCTGGCTGCCTGTGAAGGTGCATTGCTGGTGGTCGACGCCGGGCAGGG
CGTAGAAGCGCAAACCCTGGCAAACTGCTACACCGCCATGGAAATGGATCTCGAAGTTGTGCCGGTACTGAACAAGATTG
ACCTGCCGGCAGCCGATCCTGAACGCGTGGCGGAAGAAATTGAAGATATCGTCGGCATCGACGCCACCGACGCGGTGCGC
TGTTCAGCGAAAACCGGCGTTGGTGTGCAGGACGTTCTCGAACGTCTGGTGCGCGACATTCCGCCGCCGGAAGGCGATCC
GGAAGGCCCGTTGCAGGCACTAATTATCGACTCATGGTTCGACAACTACCTGGGCGTTGTTTCACTTATCCGTATTAAAA
ACGGCACCCTGCGTAAGGGCGACAAAGTGAAAGTCATGAGTACCGGGCAGACCTATAACGCCGACCGTCTGGGCATCTTC
ACGCCGAAACAGGTTGACCGCACTGAACTGAAATGTGGCGAAGTAGGCTGGCTCGTATGTGCGATTAAAGATATCCACGG
CGCTCCAGTCGGCGATACCTTAACGCTGGCGCGTAATCCGGCAGAAAAGGCGCTGCCTGGCTTTAAGAAAGTCAAACCGC
AGGTATACGCCGGTCTGTTCCCGGTAAGTTCCGACGACTATGAAGCCTTCCGTGACGCGCTGGGTAAACTCAGCCTGAAC
GATGCCTCACTGTTCTATGAGCCGGAAAGCTCCAGCGCGCTGGGCTTTGGTTTCCGCTGCGGCTTCCTCGGCCTGCTGCA
CATGGAGATCATCCAGGAACGTCTGGAACGTGAATACGATCTGGATCTGATCACCACTGCGCCGACCGTAGTGTATGAAG
TTGAAACCACGTCAAGAGAAGTTATCTACGTCGACAGCCCATCCAAGCTGCCTGCGGTAAATAACATCTACGAACTGCGC
GAGCCGATTGCAGAGTGTCACATGCTGCTGCCGCAGGCATATCTCGGCAACGTTATTACGTTGTGCGTAGAAAAACGCGG
CGTGCAGACCAATATGGTTTACCACGGTAATCAGGTGGCGCTGACGTACGAGATCCCGATGGCGGAAGTGGTGCTCGACT
TCTTCGATCGCCTGAAATCTACCTCGCGTGGTTATGCGTCTCTGGATTACAACTTCAAGCGCTTCCAGGCGTCCGACATG
GTACGTGTAGACGTATTAATCAACGGTGAACGTGTTGATGCGCTGGCGTTGATCACCCACCGTGATAATTCGCAAAACCG
CGGTCGCGAGTTGGTGGAGAAGATGAAAGATCTGATCCCACGCCAGCAGTTTGATATCGCCATTCAGGCAGCGATTGGTA
CGCACATCATTGCGCGATCCACCGTGAAACAGCTGCGTAAAAACGTACTGGCTAAATGTTATGGCGGCGATATCAGCCGT
AAGAAAAAGCTGCTGCAGAAGCAGAAAGAAGGTAAGAAACGCATGAAGCAGATCGGTAACGTCGAGCTGCCGCAGGAAGC
GTTCCTCGCCATTCTGCACGTCGGCAAAGACAACAAATAA

Upstream 100 bases:

>100_bases
GTGTAGAATGCGGCGTTTCTATTAATACAGACGTTAAGCTCAGAACAGCGACTTCTAAAAGCCTGGTTAACCGGGCATTA
AGGCACAATAATCATACTTT

Downstream 100 bases:

>100_bases
CCCTTAGGAGTTGGCATGGCGAATATGTTTGCCCTGATTCTGGTGATTGCCACACTGGTGACGGGCATTTTATGGTGCGT
GGATAAATTCTTTTTCGCAC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELR
EPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK

Sequences:

>Translated_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELR
EPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
>Mature_599_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKASDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLN
DASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELR
EPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=47.5873544093178, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=44.2953020134228, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=43.421052631579, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=43.421052631579, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI18390331, Length=152, Percent_Identity=36.1842105263158, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=32.2175732217573, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=32.2175732217573, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=32.3353293413174, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=32.3353293413174, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI34147630, Length=267, Percent_Identity=28.4644194756554, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=100, Blast_Score=1234, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=29.2490118577075, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=35.4838709677419, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789559, Length=231, Percent_Identity=29.4372294372294, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790412, Length=327, Percent_Identity=24.4648318042813, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789737, Length=327, Percent_Identity=24.4648318042813, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=613, Percent_Identity=40.7830342577488, Blast_Score=486, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=469, Percent_Identity=25.1599147121535, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=35.8823529411765, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=225, Percent_Identity=30.6666666666667, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.9234608985025, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.75, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.75, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.8131868131868, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=39.5833333333333, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=26.4227642276423, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=283, Percent_Identity=24.7349823321555, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=283, Percent_Identity=24.7349823321555, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=42.5956738768719, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=43.4782608695652, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=35.1851851851852, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=245, Percent_Identity=30.2040816326531, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=143, Percent_Identity=33.5664335664336, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17864358, Length=143, Percent_Identity=33.5664335664336, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66571; Mature: 66571

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVQDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCCEEEEEEEECCCCCCCCCEEEECCCCHHHHCCCHHHCCCHHHEEEE
PVSSDDYEAFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELREPIAECHMLLPQAYLGNVIT
CEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARS
EEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHCEEEEEHHHHHHHHHH
TVKQLRKNVLAKCYGGDISRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVQDVLERLVRDI
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLF
CCCCCCHHHCCCCCCEEEEEEEECCCCCCCCCEEEECCCCHHHHCCCHHHCCCHHHEEEE
PVSSDDYEAFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELREPIAECHMLLPQAYLGNVIT
CEEEECCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARS
EEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHCEEEEEHHHHHHHHHH
TVKQLRKNVLAKCYGGDISRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA