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The map label for this gene is rnc

Identifier: 157162046

GI number: 157162046

Start: 2741719

End: 2742399

Strand: Reverse

Name: rnc

Synonym: EcHS_A2722

Alternate gene names: 157162046

Gene position: 2742399-2741719 (Counterclockwise)

Preceding gene: 157162047

Following gene: 157162045

Centisome position: 59.06

GC content: 50.51

Gene sequence:

>681_bases
ATGAACCCCATCGTAATTAATCGGCTTCAACGGAAGCTGGGCTACACTTTTAATCATCAGGAACTGTTGCAGCAGGCATT
AACTCATCGTAGTGCCAGCAGTAAACATAACGAGCGTTTAGAATTTTTAGGCGACTCTATTCTGAGCTACGTTATCGCCA
ATGCGCTTTATCACCGTTTCCCTCGTGTGGATGAAGGCGATATGAGCCGGATGCGCGCCACGCTGGTCCGTGGCAATACG
CTGGCGGAACTGGCGCGCGAATTTGAGTTAGGCGAGTGCTTACGTTTAGGGCCAGGTGAACTTAAAAGCGGTGGATTTCG
TCGTGAGTCAATTCTCGCCGACACCGTCGAAGCATTAATTGGTGGCGTATTCCTCGATAGTGATATTCAAACCGTCGAGA
AATTAATCCTCAACTGGTATCAAACTCGTTTGGACGAAATTAGCCCAGGCGATAAACAAAAAGATCCGAAAACGCGCTTG
CAAGAATATTTGCAGGGTCGCCATCTGCCGCTGCCGACTTATCTGGTAGTCCAGGTACGTGGCGAAGCGCACGATCAGGA
ATTTACTATCCACTGCCAGGTCAGCGGCCTGAGTGAACCGGTGGTTGGCACAGGTTCAAGCCGTCGTAAGGCTGAGCAGG
CTGCCGCCGAACAGGCGTTGAAAAAACTGGAGCTGGAATGA

Upstream 100 bases:

>100_bases
TACCGGTAAACTGAAACTGCAGCGAAGCAGTTAGCAGAACCATGTATATCAGGTCTGTTTCGTGTGCTGAATTGTTGACG
CATTTATTTATTGGTATCGC

Downstream 100 bases:

>100_bases
GCATCGATAAAAGTTACTGCGGATTTATTGCCATCGTCGGACGTCCGAACGTTGGCAAATCCACATTGTTGAACAAACTG
CTGGGGCAGAAAATCTCCAT

Product: ribonuclease III

Products: NA

Alternate protein names: Ribonuclease III; RNase III

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNT
LAELAREFELGECLRLGPGELKSGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE

Sequences:

>Translated_226_residues
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNT
LAELAREFELGECLRLGPGELKSGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE
>Mature_226_residues
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNT
LAELAREFELGECLRLGPGELKSGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE

Specific function: Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs

COG id: COG0571

COG function: function code K; dsRNA-specific ribonuclease

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase III domain

Homologues:

Organism=Homo sapiens, GI155030236, Length=230, Percent_Identity=29.5652173913043, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI155030234, Length=230, Percent_Identity=29.5652173913043, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788920, Length=226, Percent_Identity=100, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17506883, Length=227, Percent_Identity=31.2775330396476, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI193202651, Length=227, Percent_Identity=31.2775330396476, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323895, Length=204, Percent_Identity=28.4313725490196, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17137682, Length=229, Percent_Identity=27.9475982532751, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNC_ECO24 (A7ZQ11)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463890.1
- ProteinModelPortal:   A7ZQ11
- SMR:   A7ZQ11
- STRING:   A7ZQ11
- EnsemblBacteria:   EBESCT00000023394
- GeneID:   5585969
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2853
- eggNOG:   COG0571
- GeneTree:   EBGT00050000011884
- HOGENOM:   HBG744556
- OMA:   ALTHKSF
- ProtClustDB:   PRK00102
- BioCyc:   ECOL331111:ECE24377A_2853-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00104
- InterPro:   IPR001159
- InterPro:   IPR014720
- InterPro:   IPR000999
- InterPro:   IPR011907
- Gene3D:   G3DSA:3.30.160.20
- Gene3D:   G3DSA:1.10.1520.10
- SMART:   SM00358
- SMART:   SM00535
- TIGRFAMs:   TIGR02191

Pfam domain/function: PF00035 dsrm; PF00636 Ribonuclease_3; SSF69065 RNase_III

EC number: =3.1.26.3

Molecular weight: Translated: 25550; Mature: 25550

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50137 DS_RBD; PS00517 RNASE_3_1; PS50142 RNASE_3_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRF
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELKSGGFRRESILADTVEALI
CCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRLQEYLQGRHLPLPTYLVVQVR
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
GEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLGDSILSYVIANALYHRF
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELKSGGFRRESILADTVEALI
CCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
GGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRLQEYLQGRHLPLPTYLVVQVR
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
GEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQALKKLELE
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA