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The map label for this gene is insB1

Identifier: 157162024

GI number: 157162024

Start: 2716921

End: 2717424

Strand: Reverse

Name: insB1

Synonym: EcHS_A2701

Alternate gene names: 157162024

Gene position: 2717424-2716921 (Counterclockwise)

Preceding gene: 157162025

Following gene: 157162023

Centisome position: 58.52

GC content: 54.76

Gene sequence:

>504_bases
ATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGCTATGTCGGGGCTAAAT
CGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGTCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAGCGCACTATG
GCGACGCTGGGGCGTCTTATGAGCCTGCTGTCACCCTTTGACGTGGTGATATGGATGACGGATGGCTGGCCGCTGTATGA
ATCCCGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGC
AGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTAT
CTGAACATAAAACACTATCAATAA

Upstream 100 bases:

>100_bases
GCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTTGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCCAACCCGTAATCTATTATTAATGGCTACGCTATTAGGTAGCGCACTATTTGCCAGGGCTGCGGAT
AAAGAAATGACCATTGGTGC

Product: IS1, transposase orfB

Products: NA

Alternate protein names: IS1a

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTM
ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ

Sequences:

>Translated_167_residues
MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTM
ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LNIKHYQ
>Mature_166_residues
PGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMA
TLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
NIKHYQ

Specific function: Absolutely required for transposition of IS1

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family

Homologues:

Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=100, Blast_Score=348, Evalue=1e-97,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=100, Blast_Score=348, Evalue=1e-97,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=100, Blast_Score=348, Evalue=1e-97,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=97.0059880239521, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1786470, Length=167, Percent_Identity=96.4071856287425, Blast_Score=335, Evalue=9e-94,
Organism=Escherichia coli, GI1786459, Length=167, Percent_Identity=96.4071856287425, Blast_Score=335, Evalue=9e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INSB1_ECOLI (P0CF25)

Other databases:

- EMBL:   J01729
- EMBL:   X52534
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JN0135
- RefSeq:   AP_000685.1
- RefSeq:   AP_002888.1
- RefSeq:   AP_004347.1
- RefSeq:   NP_414562.1
- RefSeq:   NP_416407.1
- RefSeq:   NP_417902.1
- ProteinModelPortal:   P0CF25
- EnsemblBacteria:   EBESCT00000005071
- EnsemblBacteria:   EBESCT00000015770
- GeneID:   944743
- GeneID:   945867
- GeneID:   947949
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0020
- KEGG:   eco:b0021
- EchoBASE:   EB4700
- EcoGene:   EG40002
- eggNOG:   COG1662
- GeneTree:   EBGT00070000031710
- HOGENOM:   HBG468514
- InterPro:   IPR005063

Pfam domain/function: PF03400 Transposase_27

EC number: NA

Molecular weight: Translated: 19565; Mature: 19434

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
YAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEE
YAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503