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The map label for this gene is iscU

Identifier: 157162004

GI number: 157162004

Start: 2697422

End: 2697808

Strand: Reverse

Name: iscU

Synonym: EcHS_A2680

Alternate gene names: 157162004

Gene position: 2697808-2697422 (Counterclockwise)

Preceding gene: 157162005

Following gene: 157162003

Centisome position: 58.1

GC content: 49.87

Gene sequence:

>387_bases
ATGGCTTACAGCGAAAAAGTTATCGACCATTACGAGAATCCGCGTAACGTGGGTTCCTTTGACAACAACGACGAGAACGT
CGGCAGCGGCATGGTGGGTGCACCGGCCTGTGGCGACGTGATGAAGTTGCAGATTAAAGTCAACGATGAAGGTATCATTG
AAGACGCGCGTTTTAAAACTTACGGCTGCGGTTCCGCTATCGCTTCCAGCTCCCTGGTCACCGAATGGGTGAAAGGGAAG
TCTCTCGACGAAGCGCAGGCGATCAAAAACACCGATATTGCTGAAGAACTTGAACTGCCGCCGGTGAAAATTCACTGTTC
TATTCTGGCAGAAGACGCGATCAAAGCCGCCATTGCGGACTATAAAAGCAAACGTGAAGCAAAATAA

Upstream 100 bases:

>100_bases
TGACCTTTCTCCGCTGTGGGAAATGTACAAGCAGGGCGTGGATCTGAACAGCATCGAATGGGCTCATCATTAATCGGTAT
CGGAATCAGGAGAATTTATA

Downstream 100 bases:

>100_bases
GAGTTGAGGTTTGGTTATGTCGATTACACTGAGCGACAGTGCAGCAGCGCGAGTAAATACCTTTCTGGCTAACCGCGGTA
AAGGGTTTGGCCTGCGTCTG

Product: scaffold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGK
SLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Sequences:

>Translated_128_residues
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGK
SLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK
>Mature_127_residues
AYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKS
LDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIADYKSKREAK

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential)

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family

Homologues:

Organism=Homo sapiens, GI56699456, Length=126, Percent_Identity=75.3968253968254, Blast_Score=207, Evalue=2e-54,
Organism=Homo sapiens, GI24307953, Length=126, Percent_Identity=74.6031746031746, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1788878, Length=128, Percent_Identity=100, Blast_Score=262, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17543474, Length=126, Percent_Identity=69.8412698412698, Blast_Score=193, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=72.5806451612903, Blast_Score=193, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324800, Length=124, Percent_Identity=74.1935483870968, Blast_Score=193, Evalue=7e-51,
Organism=Drosophila melanogaster, GI21355597, Length=126, Percent_Identity=74.6031746031746, Blast_Score=204, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFU_ECO57 (P0ACD6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91053
- PIR:   G85897
- RefSeq:   NP_289086.1
- RefSeq:   NP_311422.1
- PDB:   3LVL
- PDBsum:   3LVL
- ProteinModelPortal:   P0ACD6
- SMR:   P0ACD6
- EnsemblBacteria:   EBESCT00000028101
- EnsemblBacteria:   EBESCT00000060422
- GeneID:   915163
- GeneID:   957837
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3796
- KEGG:   ecs:ECs3395
- GeneTree:   EBGT00050000011269
- HOGENOM:   HBG584215
- OMA:   FTNPRNV
- ProtClustDB:   PRK11325
- BioCyc:   ECOL83334:ECS3395-MONOMER
- InterPro:   IPR011339
- InterPro:   IPR002871
- PANTHER:   PTHR10093
- TIGRFAMs:   TIGR01999

Pfam domain/function: PF01592 NifU_N

EC number: NA

Molecular weight: Translated: 13849; Mature: 13717

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHH
YKSKREAK
HHHHCCCC
>Mature Secondary Structure 
AYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQIKVNDEGIIEDARFKT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCC
YGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPVKIHCSILAEDAIKAAIAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHH
YKSKREAK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796