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The map label for this gene is guaB

Identifier: 157161983

GI number: 157161983

Start: 2670244

End: 2671710

Strand: Reverse

Name: guaB

Synonym: EcHS_A2659

Alternate gene names: 157161983

Gene position: 2671710-2670244 (Counterclockwise)

Preceding gene: 157161985

Following gene: 157161982

Centisome position: 57.54

GC content: 54.26

Gene sequence:

>1467_bases
ATGCTACGTATCGCTAAAGAAGCTCTGACGTTTGACGACGTTCTCCTCGTTCCTGCTCATTCTACCGTTCTGCCGAATAC
TGCTGACCTCAGCACCCAGCTGACGAAAACTATTCGTCTGAATATCCCTATGCTTTCCGCAGCAATGGATACCGTAACGG
AAGCGCGCCTGGCTATTGCTCTGGCTCAGGAAGGCGGTATCGGCTTTATCCACAAAAACATGTCCATTGAACGCCAGGCA
GAAGAAGTTCGCCGTGTGAAAAAACACGAATCTGGTGTGGTGACTGATCCGCAGACTGTTCTGCCAACCACGACGCTGCG
CGAAGTGAAAGAACTGACCGAGCGTAACGGTTTTGCGGGCTATCCGGTCGTTACCGAAGAAAACGAACTGGTGGGTATTA
TCACCGGTCGTGACGTGCGTTTTGTTACCGACCTGAACCAGCCGGTTAGCGTTTACATGACGCCGAAAGAGCGTCTGGTC
ACCGTGCGTGAAGGTGAAGCCCGTGAAGTGGTGCTGGCAAAAATGCACGAAAAACGCGTTGAAAAAGCGCTGGTGGTTGA
TGACGAATTCCACCTGATCGGCATGATCACCGTGAAAGATTTCCAGAAAGCGGAACGTAAACCGAACGCCTGTAAAGACG
AGCAAGGCCGTCTGCGTGTTGGTGCTGCAGTTGGCGCAGGTGCGGGTAACGAAGAGCGTGTTGACGCGCTGGTTGCCGCT
GGCGTTGACGTTCTGCTGATCGACTCCTCCCACGGTCACTCAGAAGGCGTTTTGCAGCGTATCCGTGAAACCCGTGCTAA
ATATCCGGATCTGCAAATCATCGGCGGCAACGTGGCAACAGCTGCAGGTGCACGCGCTTTGGCAGAAGCTGGTTGCAGCG
CGGTTAAAGTCGGTATCGGCCCTGGTTCTATCTGTACTACTCGTATCGTTACTGGCGTAGGTGTTCCGCAGATCACTGCC
GTTGCTGACGCAGTTGAAGCGCTGGAAGGCACAGGTATTCCGGTTATCGCAGATGGTGGTATTCGCTTCTCCGGCGACAT
CGCCAAAGCTATCGCCGCTGGCGCAAGCGCGGTAATGGTGGGTTCCATGCTGGCGGGTACTGAAGAATCTCCGGGTGAAA
TCGAACTCTACCAGGGCCGTTCTTACAAATCTTACCGTGGTATGGGTTCCCTGGGCGCGATGTCCAAAGGTTCCTCTGAC
CGTTATTTCCAGAGCGATAACGCTGCCGACAAACTGGTGCCGGAAGGTATCGAAGGTCGCGTAGCTTATAAAGGTCGCCT
GAAAGAGATCATTCACCAGCAGATGGGTGGCCTGCGCTCCTGTATGGGCCTGACCGGCTGTGGTACTATCGACGAACTGC
GTACTAAAGCGGAGTTTGTACGTATCAGCGGTGCGGGCATTCAGGAAAGCCACGTTCACGACGTGACCATTACTAAAGAG
TCCCCGAACTACCGTCTGGGCTCCTGA

Upstream 100 bases:

>100_bases
GATAGCAAGCATTTTTTGCAAAAAGGGGTAGATGCAATCGGTTACGCTCTGTATAATGCCGCGGCAATATTTATTAACCA
CTCTGGTCGAGATATTGCCC

Downstream 100 bases:

>100_bases
TTCTCTTCGCCCGACTTCATGTCGGGCGATTTATATTATCTGTTTCACTTGCCTCGGAATAAGCGTCAATGACGGAAAAC
ATTCATAAGCATCGCATCCT

Product: inosine 5'-monophosphate dehydrogenase

Products: NA

Alternate protein names: IMP dehydrogenase; IMPD; IMPDH

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQA
EEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRLRVGAAVGAGAGNEERVDALVAA
GVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITA
VADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSD
RYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKAEFVRISGAGIQESHVHDVTITKE
SPNYRLGS

Sequences:

>Translated_488_residues
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQA
EEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRLRVGAAVGAGAGNEERVDALVAA
GVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITA
VADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSD
RYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKAEFVRISGAGIQESHVHDVTITKE
SPNYRLGS
>Mature_488_residues
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQA
EEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRLRVGAAVGAGAGNEERVDALVAA
GVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITA
VADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSD
RYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKAEFVRISGAGIQESHVHDVTITKE
SPNYRLGS

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI66933016, Length=460, Percent_Identity=41.9565217391304, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI217035146, Length=466, Percent_Identity=41.4163090128755, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI34328928, Length=466, Percent_Identity=41.4163090128755, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI156616279, Length=466, Percent_Identity=41.4163090128755, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI34328930, Length=466, Percent_Identity=41.4163090128755, Blast_Score=325, Evalue=8e-89,
Organism=Homo sapiens, GI217035152, Length=455, Percent_Identity=41.7582417582418, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI217035148, Length=466, Percent_Identity=40.5579399141631, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI217035150, Length=466, Percent_Identity=37.9828326180258, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI156104880, Length=267, Percent_Identity=34.4569288389513, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI50541954, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541952, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541948, Length=247, Percent_Identity=36.0323886639676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50541956, Length=247, Percent_Identity=36.0323886639676, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788855, Length=488, Percent_Identity=100, Blast_Score=985, Evalue=0.0,
Organism=Escherichia coli, GI1786293, Length=221, Percent_Identity=36.6515837104072, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994385, Length=478, Percent_Identity=36.4016736401674, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71994389, Length=423, Percent_Identity=38.7706855791962, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17560440, Length=219, Percent_Identity=36.986301369863, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322012, Length=464, Percent_Identity=39.8706896551724, Blast_Score=322, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6323585, Length=465, Percent_Identity=40, Blast_Score=318, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6323464, Length=448, Percent_Identity=41.5178571428571, Blast_Score=313, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6319352, Length=342, Percent_Identity=39.766081871345, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319353, Length=119, Percent_Identity=42.8571428571429, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24641071, Length=487, Percent_Identity=41.2731006160164, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24641073, Length=487, Percent_Identity=41.2731006160164, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI28571163, Length=445, Percent_Identity=41.7977528089888, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): IMDH_ECO57 (P0ADG8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91050
- PIR:   G85894
- RefSeq:   NP_289062.1
- RefSeq:   NP_311397.1
- ProteinModelPortal:   P0ADG8
- SMR:   P0ADG8
- EnsemblBacteria:   EBESCT00000025403
- EnsemblBacteria:   EBESCT00000058592
- GeneID:   917779
- GeneID:   957789
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3772
- KEGG:   ecs:ECs3370
- GeneTree:   EBGT00050000009443
- HOGENOM:   HBG298985
- OMA:   CVGLITV
- ProtClustDB:   PRK05567
- BioCyc:   ECOL83334:ECS3370-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR005990
- InterPro:   IPR018529
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000130
- SMART:   SM00116
- TIGRFAMs:   TIGR01302

Pfam domain/function: PF00571 CBS; PF00478 IMPDH

EC number: =1.1.1.205

Molecular weight: Translated: 52023; Mature: 52023

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS51371 CBS; PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 305-305 BINDING 338-338 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIA
CCEEHHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEEEEECCCHHHHHHHHHHHHHEEEE
LAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAG
EECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCC
YPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRV
CCEEECCCCEEEEEECCCEEEEECCCCCEEEEECCHHHEEEEECCCCHHHHHHHHHHHHH
EKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRLRVGAAVGAGAGNEERVDALVAA
HHEEEECCCEEEEEEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHC
GVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKVGIG
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECC
PGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMV
CCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCHHHHHHHCCCHHHHH
GSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
HHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCE
VAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKAEFVRISGAGIQESHVHDVTITKE
EEEHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEEEEEEC
SPNYRLGS
CCCCCCCC
>Mature Secondary Structure
MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTEARLAIA
CCEEHHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEEEEECCCHHHHHHHHHHHHHEEEE
LAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLPTTTLREVKELTERNGFAG
EECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCC
YPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLVTVREGEAREVVLAKMHEKRV
CCEEECCCCEEEEEECCCEEEEECCCCCEEEEECCHHHEEEEECCCCHHHHHHHHHHHHH
EKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRLRVGAAVGAGAGNEERVDALVAA
HHEEEECCCEEEEEEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHC
GVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGGNVATAAGARALAEAGCSAVKVGIG
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECC
PGSICTTRIVTGVGVPQITAVADAVEALEGTGIPVIADGGIRFSGDIAKAIAAGASAVMV
CCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCHHHHHHHCCCHHHHH
GSMLAGTEESPGEIELYQGRSYKSYRGMGSLGAMSKGSSDRYFQSDNAADKLVPEGIEGR
HHHHCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCE
VAYKGRLKEIIHQQMGGLRSCMGLTGCGTIDELRTKAEFVRISGAGIQESHVHDVTITKE
EEEHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEEEEEEC
SPNYRLGS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796