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The map label for this gene is hda [H]

Identifier: 157161957

GI number: 157161957

Start: 2640012

End: 2640758

Strand: Reverse

Name: hda [H]

Synonym: EcHS_A2631

Alternate gene names: 157161957

Gene position: 2640758-2640012 (Counterclockwise)

Preceding gene: 157161958

Following gene: 157161956

Centisome position: 56.87

GC content: 53.01

Gene sequence:

>747_bases
GTGGTAAACTTCTCGCGATTTTGTGAAATCCTGGTTGAGGTATCTCTGAACACACCGGCACAGCTCTCTTTGCCACTTTA
TCTTCCTGACGACGAAACCTTTGCAAGTTTCTGGCCGGGGGATAACTCCTCTTTACTGGCCGCGCTGCAAAACGTGCTGC
GTCAGGAACATAGCGGTTACATCTATCTCTGGGCACGCGAAGGCGCGGGGCGCAGCCATCTGCTGCACGCGGCTTGCGCG
GAATTGTCGCAGCGTGGCGATGCGGTGGGCTATGTCCCGCTGGATAAACGCACCTGGTTTGTTCCGGAAGTGCTCGACGG
TATGGAGCATTTGTCGCTGGTCTGTATCGACAACATTGAGTGTATTGCAGGCGATGAGTTGTGGGAGATGGCGATTTTCG
ATCTCTACAATCGAATTCTGGAATCGGGCAAAACACGATTGTTGATCACCGGCGATCGTCCACCGCGGCAGTTGAATCTG
GGATTACCGGATCTCGCGTCGCGACTCGACTGGGGGCAGATCTACAAATTGCAGCCACTTTCTGATGAAGATAAGTTGCA
GGCGCTACAGTTACGCGCGCGTTTGCGTGGTTTTGAACTGCCGGAAGATGTGGGGCGTTTCTTGCTGAAGCGGCTCGACA
GAGAAATGCGCACGCTATTTATGACGTTGGATCAGTTGGATCGTGCGTCGATTACCGCGCAACGTAAGCTGACCATTCCG
TTTGTGAAAGAAATTCTGAAGTTGTAG

Upstream 100 bases:

>100_bases
CCTGAAGAAGTGGTGCTGGACGCAGAAGACGCGGATATAACAGACAAATAATGTACCGACCGGGCAGTGTTGCTGCCCGG
TTCAAACATCATGGGATTCT

Downstream 100 bases:

>100_bases
ATAAGGTGTTTATTGTCGGATGCGGCGCGAACGCCTTATCCGAACTACGCGAAGTTAATCCATAGTATTCGTAGGCCGGA
TAAGACGTGGCAAGCGTCGC

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACA
ELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIP
FVKEILKL

Sequences:

>Translated_248_residues
MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACA
ELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIP
FVKEILKL
>Mature_248_residues
MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACA
ELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIP
FVKEILKL

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=233, Percent_Identity=99.5708154506438, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI2367267, Length=250, Percent_Identity=27.6, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 28370; Mature: 28370

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGY
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCE
IYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHEEEEEECCCE
CIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPL
EECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCC
SDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECH
FVKEILKL
HHHHHHCC
>Mature Secondary Structure
MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGY
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCE
IYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHEEEEEECCCE
CIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPL
EECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCC
SDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECH
FVKEILKL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA