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The map label for this gene is eutR [H]

Identifier: 157161904

GI number: 157161904

Start: 2582827

End: 2583879

Strand: Reverse

Name: eutR [H]

Synonym: EcHS_A2574

Alternate gene names: 157161904

Gene position: 2583879-2582827 (Counterclockwise)

Preceding gene: 157161905

Following gene: 157161901

Centisome position: 55.64

GC content: 53.28

Gene sequence:

>1053_bases
ATGAAAAAGACCCGTACAGCCAATTTGCACCATCTTTATCATGAACCCTTACCCGAAAACCTGAAGCTCACGCCGAAGGT
CGAAGTGGATAATGTTCATCAACGACAGACAACGGATGTCTATGAACATGCTTTAACGATTACCGCCTGGCAGCAGATTT
ACGATCAGCTGCATCCGGGCAAGTTTCATGGTGAATTTACGGAAATTCTACTCGATGATATTCAGGTTTTTCGTGAATAC
ACCGGTCTGGCGCTGCGTCAGTCGTGCCTGGTCTGGCCGAACTCGTTCTGGTTTGGCATTCCGGCGACGCGCGGTGAGCA
GGGATTTATCGGTTCGCAATGTCTGGGAAGCGTGGAAATCGCCACCCGCCCTGGTGGCACTGAATTTGAACTGAGCACGC
CGGATGATTACACGATCCTGGGCGTGGTGCTTTCTGAAGATGTCATCACCCGGCAGGCTAACTTTTTGCATAACCCGGAT
CGGGTATTACATATGTTGCGTAACCAGTCGGCGCTGGAAGTGAAAGAGCAGCATAAAGCCGCGCTGTGGGGCTTTGTCCA
ACAGGCGCTGGCGACGTTTTGCGAGAATCCGGAAAATCTCCATCAGCCTGCAGTGCGAAAAGTGCTGGGGGATAATTTGC
TAATGGCGATGGGGGCCATGCTGGAAGAAGCGCAACCAATGGTGACGGCGGAAAGCATCAGTCATCAGAGTTACCGTCGA
TTGCTTTCCCGCGCCCGTGAATATGTGCTGGAAAACATGTCCGAACCGGTGACGGTGCTGGATTTGTGTAATCAACTGCA
TGTCAGCCGCCGCACGCTACAAAACGCGTTTCACGCTATTTTAGGCATTGGCCCGAACGCGTGGCTGAAACGCATTCGCC
TGAACGCCGTACGCCGCGAACTGATAAGTCCGTGGTCGCAAAGTATGACGGTAAAAGACGCCGCCATGCAGTGGGGATTC
TGGCATCTGGGGCAATTTGCCACGGATTACCAGCAGCTGTTTTCCGAGAAGCCGTCACTGACGCTGCATCAGCGGATGCG
GGAGTGGGGGTGA

Upstream 100 bases:

>100_bases
TTCTGCCGGGACGTTACGTAAACGCAGTAGTCGTTATCGTCTCAAGCCCCATTAACCTGTCGGAGGTGCCGGGTGTCCTA
CAACACCCGGCATTAACATC

Downstream 100 bases:

>100_bases
GGGAGTTACACCCAATCCCTGACCTTAATAAACTCACTTAACGCCGCTTCTGGGCTGCCATCTTTTGGCTGATAATCATA
TTCCCAGCGTACCAGTGGCG

Product: transcriptional regulator EutR

Products: NA

Alternate protein names: Ethanolamine operon regulatory protein [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSVEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFSEKPSLTLHQRMREWG

Sequences:

>Translated_350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSVEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFSEKPSLTLHQRMREWG
>Mature_350_residues
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREY
TGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSVEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
RVLHMLRNQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSYRR
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGF
WHLGQFATDYQQLFSEKPSLTLHQRMREWG

Specific function: Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=350, Percent_Identity=99.7142857142857, Blast_Score=730, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 40188; Mature: 40188

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPG
CCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSVEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCEEE
ATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPDRVLHMLRNQSALEVKEQHKA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
ALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSYRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLFSEKPSLTLHQRMREWG
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHALTITAWQQIYDQLHPG
CCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPATRGEQGFIGSQCLGSVEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHCCCEEE
ATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPDRVLHMLRNQSALEVKEQHKA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHH
ALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMGAMLEEAQPMVTAESISHQSYRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNAFHAILGIGPNAWLKRIRLNAVRRE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLFSEKPSLTLHQRMREWG
HHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503 [H]