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The map label for this gene is cysT [H]

Identifier: 157161890

GI number: 157161890

Start: 2569243

End: 2570076

Strand: Reverse

Name: cysT [H]

Synonym: EcHS_A2560

Alternate gene names: 157161890

Gene position: 2570076-2569243 (Counterclockwise)

Preceding gene: 157161891

Following gene: 157161889

Centisome position: 55.35

GC content: 55.4

Gene sequence:

>834_bases
ATGTTTGCGGTTTCTTCAAGACGCGTGCTGCCGGGCTTTACCTTAAGCCTCGGCACCAGTCTGCTGTTTGTTTGCCTGAT
TTTGCTGCTGCCACTCTCCGCGCTGGTGATGCAGCTGGCCCAGATTAGCTGGGCGCAGTACTGGGAGGTGATCACCAACC
CGCAGGTGGTCGCAGCCTACAAAGTGACGCTGCTGTCGGCGTTTGTGGCATCGATTTTTAACGGCGTTTTCGGTCTGCTG
ATGGCGTGGATCTTAACTCGTTATCGCTTCCCTGGCCGCACGCTGCTTGATGCGCTGATGGATTTACCCTTTGCGCTGCC
AACGGCAGTCGCCGGTTTAACGCTGGCCTCGCTCTTTTCTGTAAACGGTTTTTACGGTGAATGGCTGGCGAAGTTTGATA
TCAAAGTCACCTATACCTGGCTGGGGATTGCGGTGGCGATGGCCTTTACCAGCATTCCGTTTGTGGTACGTACCGTGCAG
CCGGTGCTGGAAGAGTTAGGCCCGGAATATGAAGAAGCGGCGGAAACACTTGGTGCAACGCGCTGGCAGAGTTTCTGCAA
AGTGGTGCTGCCGGAGCTTTCTCCGGCGCTGGTGGCGGGCGTGGCGCTGTCGTTTACCCGTAGTCTTGGTGAATTTGGCG
CGGTGATTTTTATCGCCGGAAATATCGCGTGGAAGACGGAAGTGACGTCGCTGATGATTTTTGTGCGCTTGCAGGAGTTT
GATTACCCGGCGGCGAGCGCGATTGCTTCGGTGATCCTCGCGGCATCCCTGATACTGCTGTTCTCAATTAACACTCTGCA
AAGTCGCTTTGGTCGGCGTGTGGTAGGTCATTAA

Upstream 100 bases:

>100_bases
TCCGCGTGGAAGACAAATTTGGCTCCTGGCCGGAAGTGATGAAAACCCACTTCACCAGCGGCGGCGAGTTAGACAAGCTG
TTAGCGGCGGGGCGTAATTA

Downstream 100 bases:

>100_bases
TGGCGGAAGTGACACAATTGAAGCGTTATGACGCGCGCCCGATTAACTGGGGCAAATGGTTTCTGATTGGCATCGGGATG
CTGGTTTCGGCGTTCATCCT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQISWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLILLFSINTLQSRFGRRVVGH

Sequences:

>Translated_277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQISWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLILLFSINTLQSRFGRRVVGH
>Mature_277_residues
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQISWAQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLL
MAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLILLFSINTLQSRFGRRVVGH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=277, Percent_Identity=99.2779783393502, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=35.5212355212355, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=34.4827586206897, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787368, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30274; Mature: 30274

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQISWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLILLFSINTLQSRFGRRVVGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQISWAQYWEVITNPQVVAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDLPFALPTAVAGLTLASLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLILLFSINTLQSRFGRRVVGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]