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The map label for this gene is ligA [H]

Identifier: 157161876

GI number: 157161876

Start: 2555725

End: 2557740

Strand: Reverse

Name: ligA [H]

Synonym: EcHS_A2546

Alternate gene names: 157161876

Gene position: 2557740-2555725 (Counterclockwise)

Preceding gene: 157161877

Following gene: 157161875

Centisome position: 55.08

GC content: 54.61

Gene sequence:

>2016_bases
ATGGAATCAATCGAACAACAACTGACAGAACTGCGAACGACGCTTCGCCATCATGAATATCTTTATCATGTGATGGATGC
GCCGGAAATTCCCGACGCTGAATACGACAGGCTGATGCGCGAACTGCGCGAGCTGGAAACCAAACATCCAGAACTGATTA
CGCCTGATTCGCCTACCCAACGTGTAGGTGCTGCGCCGCTGGCGGCTTTCAGCCAGATTCGCCATGAAGTGCCAATGCTG
TCGCTGGATAACGTTTTTGATGAAGAAAGCTTTCTTGCCTTCAACAAACGAGTGCAGGACCGTCTGAAAAGCAACGAGAA
AGTCACCTGGTGCTGTGAGCTGAAGCTGGATGGCCTTGCTGTCAGTATTCTGTATGAAAATGGCGTTTTAGTCAGTGCCG
CGACCCGTGGCGATGGCACCACCGGGGAAGATATCACGTCTAACGTGCGTACCATTCGCGCCATTCCACTGAAGCTACAC
GGAGAGAATATCCCGGCGCGTCTGGAAGTGCGTGGTGAAGTGTTCCTGCCGCAGGCGGGGTTCGAAAAGATTAACGAAGA
TGCGCGACGCACGGGCGGGAAAGTTTTTGCTAACCCACGTAATGCGGCAGCTGGTTCACTGCGTCAGCTTGATCCGCGTA
TTACAGCGAAGCGACCGCTCACTTTTTTCTGCTATGGCGTTGGTGTTCTGGAAGGTGGCGAGCTGCCGGATACTCATCTT
GGCCGTTTACTGCAATTTAAACAGTGGGGGTTGCCGGTCAGCGATCGGGTAACGCTTTGTGAATCGGCGGAAGAAGTGCT
GGCGTTCTATCACAAAGTGGAAGAAGACCGCCCGACGCTGGGCTTTGATATCGACGGCGTGGTGATTAAGGTCAACTCAC
TGGCACAGCAGGAGCAGCTTGGCTTTGTCGCGCGTGCCCCGCGCTGGGCGGTAGCGTTTAAATTCCCGGCGCAGGAGCAG
ATGACCTTTGTGCGTGACGTCGAGTTTCAGGTTGGGCGTACTGGCGCGATTACGCCTGTTGCGCGTCTGGAACCTGTCCA
TGTTGCAGGCGTGCTGGTGAGTAACGCAACCTTACACAATGCGGATGAAATCGAACGTCTTGGTTTACGCATTGGCGATA
AAGTGGTGATTCGCCGCGCTGGCGACGTGATCCCGCAGGTGGTTAACGTCGTGCTTTCTGAACGCCCGGAAGATACCCGT
GAGGTTGTATTCCCGACGCATTGTCCGGTATGTGGTTCTGACGTTGAGCGTGTGGAAGGTGAAGCGGTTGCCCGCTGTAC
CGGTGGCCTGATTTGCGGTGCGCAGCGTAAAGAGTCGCTGAAACACTTTGTTTCCCGCCGTGCGATGGATGTTGACGGAA
TGGGCGACAAAATCATCGATCAGCTGGTTGAAAAAGAATATGTCCATACTCCGGCAGATCTGTTCAAACTCACCGCAGGC
AAACTGACCGGACTGGAGCGTATGGGGCCAAAATCGGCACAAAACGTGGTTAACGCGCTGGAAAAAGCGAAAGAAACCAC
CTTTGCTCGCTTCCTCTATGCACTTGGCATCCGTGAAGTCGGCGAGGCCACCGCAGCAGGTCTGGCGGCATATTTCGGCA
CGCTGGAAGCGCTGGAAGCCGCTTCGATTGAAGAGCTGCAAAAGGTGCCTGATGTTGGCATTGTCGTTGCATCCCACGTT
CACAACTTCTTTGCCGAAGAAAGCAACCGCAATGTCATCAGCGAGCTGTTGGCGGAAGGTGTTCACTGGCCTGCGCCGAT
CGTTATCAACGCGGAAGAGATTGACAGCCCGTTTGCTGGTAAAACCGTGGTGCTTACGGGCAGCTTAAGCCAGATGTCGC
GTGATGACGCTAAAGCTCGACTGGTCGAACTGGGCGCGAAAGTCGCGGGCAGCGTGTCGAAGAAAACCGATCTGGTGATA
GCGGGTGAAGCTGCAGGATCTAAACTGGCGAAGGCGCAGGAACTGGGCATTGAAGTCATCGACGAAGCGGAAATGCTGCG
TTTGCTGGGTAGCTGA

Upstream 100 bases:

>100_bases
ATCCGCGAAGTCAAAGACGCCAACGCCTGATACACTTAAGGCAAATTAACTCCTCTTCGAACCCCCGCTTGTCGGGGGTT
TTTAGCATTGATGGTGCGAT

Downstream 100 bases:

>100_bases
GATGGAAAAAGAGCAGCTGATTGAAATAGCCAATACGATAATGCCGTTTGGCAAATACAAAGGGCGTCGCTTAATCGACC
TGCCGGAGGAGTATCTGCTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMLRLLGS

Sequences:

>Translated_671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMLRLLGS
>Mature_671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMLRLLGS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=99.7019374068554, Blast_Score=1363, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=518, Percent_Identity=23.3590733590734, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73580; Mature: 73580

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLA
HHCCHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
CHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEG
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEA
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCHHC
DEAEMLRLLGS
CHHHHHHHHCC
>Mature Secondary Structure
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLA
HHCCHHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
CHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEG
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEA
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCHHC
DEAEMLRLLGS
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA