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The map label for this gene is ypdF [H]

Identifier: 157161858

GI number: 157161858

Start: 2536949

End: 2538034

Strand: Reverse

Name: ypdF [H]

Synonym: EcHS_A2522

Alternate gene names: 157161858

Gene position: 2538034-2536949 (Counterclockwise)

Preceding gene: 157161859

Following gene: 157161857

Centisome position: 54.66

GC content: 56.91

Gene sequence:

>1086_bases
ATGACATTACTCGCTTCGCTGCGCGACTGGCTTAAGGCGCAACAACTGGATGCAGTGCTTCTCTCCTCACGGCAGAACAA
ACAGCCGCATCTGGGGATCTCCACCGGATCAGGTTATGTGGTGATTAGCCGTGAAAGTGCGCACATTCTGGTGGATTCGC
GCTATTACGTTGAGGTGGAAGCCCGTGCGCAAGGCTACCAGCTGCATTTGCTTGACGCGACGAACACGCTTACCACTATC
GTCAATCAAATCATTGCCGATGAACAGTTGCAAACGCTCGGTTTTGAGGGCCAGCAGGTGAGTTGGGAAACCGCGCACCG
CTGGCAGTCTGAACTCAATGCGAAACTGGTTAGCGCCACGCCGGATGTGCTGCGGCAAATCAAAACGCCAGAGGAGGTGG
AGAAAATCCGCCTCGCCTGTGGGATTGCTGATCGCGGTGCAGAGCATATTCGCCGCTTTATTCAGGCGGGGATGAGCGAG
CGCGAGATAGCCGCTGAACTGGAGTGGTTTATGCGCCAGCAGGGCGCAGAAAAAGCCTCTTTTGACACCATTGTCGCCAG
TGGCTGGCGTGGGGCGCTGCCGCACGGCAAAGCCAGCGACAAGATTGTTGCAGCGGGCGAGTTTGTCACTCTCGATTTCG
GTGCGCTGTATCAGGGCTACTGCTCTGATATGACGCGCACCTTGCTGGTGAATGGCGAAGGGGTGAGCGCCGAATCTCAC
CCGCTGTTTAACGTCTATCAAATTGTCCTGCAGGCACAGCTCGCAGCAATCTCCGCGATTCGCCCCGGCGTGCGCTGCCA
GCAGGTTGACGATGCCGCGCGCCGGGTCATTACAGAAGCAGGTTATGGCGACTATTTCGGTCATAACACCGGTCACGCTA
TCGGCATTGAAGTTCATGAAGATCCGCGTTTTTCACCGCGGGACACCACGACGCTACAGCCAGGCATGTTACTGACCGTG
GAGCCGGGGATTTATTTGCCAGGGCAAGGGGGCGTGCGCATCGAAGATGTTGTGCTGGTTACCCCGCAAGGCGCAGAAGT
GCTCTACGCCATGCCGAAAACAGTGTTGCTCACGGGAGAGGCATAA

Upstream 100 bases:

>100_bases
TGGGGGCAGTCATAACTGCGCTGATGGTGGTGTTCCTGCGTCTGATGATGTTCCGTAAAGGCAAATTGTTAATCGATAGC
CTGTAAGAAGGAAAATCCAG

Downstream 100 bases:

>100_bases
TGGATTTATCGCTATTAAAAGCGTTGAGCGAGGCAGATGCGATCGCCTCCTCGGAACAGGAAGTGCGGCAGATCCTGCTG
GAAGAAGCGGATCGCCTGCA

Product: aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTI
VNQIIADEQLQTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Sequences:

>Translated_361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTI
VNQIIADEQLQTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA
>Mature_360_residues
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIV
NQIIADEQLQTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSER
EIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESHP
LFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVE
PGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Specific function: Hydrolyzes the N-terminal methionine when the next amino acid is alanine, proline or serine. The substrate preference for methionyl aminopeptidase activity is Pro > Ala > Ser. Also able to hydrolyze the Xaa-Pro peptide bond when the first amino acid is al

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

Organism=Homo sapiens, GI11559925, Length=254, Percent_Identity=30.3149606299213, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI149589008, Length=307, Percent_Identity=27.6872964169381, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI260593665, Length=307, Percent_Identity=27.6872964169381, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI260593663, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI264681563, Length=198, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI93141226, Length=174, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI264681565, Length=195, Percent_Identity=31.7948717948718, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788728, Length=361, Percent_Identity=99.7229916897507, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1789275, Length=254, Percent_Identity=29.9212598425197, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786364, Length=207, Percent_Identity=25.1207729468599, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790282, Length=300, Percent_Identity=26, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508215, Length=312, Percent_Identity=28.2051282051282, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989583, Length=250, Percent_Identity=25.2, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17509539, Length=169, Percent_Identity=33.1360946745562, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321118, Length=285, Percent_Identity=27.0175438596491, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320922, Length=245, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323273, Length=253, Percent_Identity=24.901185770751, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920384, Length=261, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357079, Length=294, Percent_Identity=27.891156462585, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137632, Length=172, Percent_Identity=33.7209302325581, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 39608; Mature: 39477

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYVEVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECEEEEEEE
ARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSWETAHRWQSELNAKLVSAT
ECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHCCEEEEEECCCCCCCCCC
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGYGDYFGHNTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEC
DPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C
>Mature Secondary Structure 
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYVEVE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECEEEEEEE
ARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSWETAHRWQSELNAKLVSAT
ECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHCCEEEEEECCCCCCCCCC
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGYGDYFGHNTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEC
DPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]