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The map label for this gene is pdxB [H]

Identifier: 157161808

GI number: 157161808

Start: 2478286

End: 2479422

Strand: Reverse

Name: pdxB [H]

Synonym: EcHS_A2471

Alternate gene names: 157161808

Gene position: 2479422-2478286 (Counterclockwise)

Preceding gene: 157161810

Following gene: 157161807

Centisome position: 53.4

GC content: 52.86

Gene sequence:

>1137_bases
GTGAAAATCCTTGTTGATGAAAATATGCCTTATGCCCGCGACTTATTTAGCCGTTTGGGTGAGGTGACCGCGGTTCCCGG
GCGTCCAATCCCCGTCGCTCAACTGGCAGACGCGGATGCGCTGATGGTGCGTTCGGTCACGAAAGTGAATGAATCTTTGC
TGGCAGGAAAACCCATTAAATTTGTTGGCACTGCCACTGCGGGGACCGACCATGTCGATGAAGCATGGTTGAAGCAGGCG
GGAATTGGTTTTTCCGCTGCACCTGGCTGTAATGCGATTGCGGTGGTGGAATATGTTTTCTCCTCACTACTGATGCTTGC
CGAACGCGATGGATTTTCACTGCACGACCGTACGGTGGGGATCGTGGGCGTTGGTAACGTTGGACGTCGATTGCAGGCGC
GACTGGAAGCGTTAGGGATTAAAACATTGCTTTGCGATCCGCCTCGCGCTGACCGTGGGGATGAGGGTGATTTCCGCTCG
CTGGATGAATTAGTTCAGCATGCGGATATTCTGACTTTCCATACGCCACTCTTCAAAGATGGGCCGTACAAAACGCTGCA
TCTGGCGGATGAAAAACTGATCCGTAGCCTGAAGCCGGGAGCGATTCTGATTAACGCCTGCCGTGGCGCAGTCGTCGATA
ATACTGCGTTGCTGACCTGCCTGAATGAAGGCCAGAAGTTAAGCGTAGTGCTGGATGTCTGGGAAGGCGAACCGGAACTT
AACGTGGAGTTGCTGACAAAAGTGGATATCGGCACGCCGCATATCGCAGGTTATACCCTGGAAGGTAAAGCACGCGGTAC
TACGCAAGTGTTTGAAGCCTATAGCAAGTTTATTGGGCATGAACAGCACGTTGCGCTGGATACATTACTGCCCGCGCCAG
AGTTTGGTCGCATTACGCTGCATGGCCCGCTCGATCAACCGACGCTGAAAAGGCTGGTGCATTTGGTGTATGATGTGCGC
CGCGATGACGCACCGCTGCGTAAAGTCGCCGGGATACCGGGTGAGTTCGATAAACTGCGCAAAAACTATCTTGAGCGCCG
TGAATGGTCATCTCTGTATGTAATTTGTGATGACGCCAGTGCGGCATCATTGCTGTGTAAACTGGGTTTTAACGCCGTTC
ATCATCCGGCACGTTAA

Upstream 100 bases:

>100_bases
ATTACGTATCCTTATACCTGAAATCTTCGCAAGTATGCCTGGCCGCGAGATTATGGCACACTTGTCCGGTTAACTCTCGT
CTCATACAGGTAACACAAAC

Downstream 100 bases:

>100_bases
TCTCTTCTTCATGCTCTCTGCTGTAACATTGGCAGGGAGCTTTGCTATTTCTGGAGTAAACCACCATGTCTGAAGGCTGG
AACATTGCCGTCCTGGGCGC

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Sequences:

>Translated_378_residues
MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR
>Mature_378_residues
MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
NVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=257, Percent_Identity=30.3501945525292, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=26.5873015873016, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=26.5873015873016, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI145580578, Length=276, Percent_Identity=25, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4557499, Length=276, Percent_Identity=25, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI6912396, Length=284, Percent_Identity=27.4647887323944, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788660, Length=378, Percent_Identity=98.9417989417989, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=205, Percent_Identity=25.8536585365854, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789279, Length=305, Percent_Identity=28.1967213114754, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082289, Length=286, Percent_Identity=28.6713286713287, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=28.1368821292776, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=323, Percent_Identity=28.4829721362229, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=323, Percent_Identity=27.5541795665635, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=286, Percent_Identity=25.8741258741259, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=308, Percent_Identity=25.6493506493506, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921140, Length=273, Percent_Identity=26.007326007326, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24646446, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646448, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646452, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646450, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574286, Length=232, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41306; Mature: 41306

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHDRTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEE
IVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNESLLAGKPIK
CEEEECCCCCHHHHHHHHHCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
FVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFSLHDRTVG
EEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCEEE
IVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRSLDELVQHADILTFHTPLFKD
EEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLNEGQKLSVVLDVWEGEPEL
CCCCEEEHHHHHHHHHCCCCEEEEECCCCCEECCCEEEEEECCCCEEEEEEEEECCCCCC
NVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHVALDTLLPAPEFGRITL
CEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKNYLERREWSSLYVICDDAS
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCH
AASLLCKLGFNAVHHPAR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA