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The map label for this gene is hisM [H]

Identifier: 157161794

GI number: 157161794

Start: 2466088

End: 2466804

Strand: Reverse

Name: hisM [H]

Synonym: EcHS_A2457

Alternate gene names: 157161794

Gene position: 2466804-2466088 (Counterclockwise)

Preceding gene: 157161795

Following gene: 157161793

Centisome position: 53.12

GC content: 49.65

Gene sequence:

>717_bases
GTGATCGAAATCTTACATGAATACTGGAAACCGCTGCTGTGGACCGACGGTTATCGCTTTACTGGTGTGGCGATCACTCT
GTGGCTGCTTATTTTGTCGGTAGTGATAGGCGGAGTCCTGGCGCTGTTTCTGGCGATTGGTCGTGTCTCCAGTAATAAAT
ACATCCAGTTTCCAATCTGGTTATTTACCTATATTTTTCGCGGTACGCCGCTGTATGTTCAGTTGCTGGTGTTCTATTCC
GGCATGTACACGCTTGAGATTGTTAAGGGAACCGAATTCCTTAACGCTTTCTTCCGCAGTGGCCTGAACTGTACCGTGCT
GGCGCTGACGCTTAACACCTGCGCTTACACTACCGAGATTTTTGCTGGGGCAATCCGTTCGGTTCCGCATGGGGAAATTG
AAGCCGCCAGAGCCTATGGCTTCTCGACTTTTAAAATGTATCGTTGCATTATTTTGCCTTCTGCGCTGCGTATTGCGTTA
CCGGCATACAGCAACGAAGTGATCCTGATGCTGCACTCTACTGCGCTGGCGTTTACGGCCACTGTGCCGGATCTGCTGAA
AATAGCCTGCGATATTAACGCCGCCACGTATCAACCTTTTACCGCCTTCGGCATTGCCGCGGTGCTCTATTTAATCATCT
CTTATGTCCTGATCAGCCTCTTTCGCAGAGCGGAAAAACGCTGGTTGCAGCATGTGAAACCTTCTTCAACGCACTGA

Upstream 100 bases:

>100_bases
TCGTCTGTGGCGTGATTTACCTGGTTTTCACCACTGTTTCCAATGGTGTGCTGCTGTTCCTTGAGCGCCGCTACTCCGTG
GGTGTGAAGAGGGCTGACCT

Downstream 100 bases:

>100_bases
GAATACGATGTCCGAGAATAAATTAAACGTTATCGATTTGCACAAACGCTACGGCAAACATGAAGTGCTGAAAGGGGTCT
CACTGCAAGCGAATGCCGGA

Product: histidine ABC transporter, permease protein HisM

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIACDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH

Sequences:

>Translated_238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIACDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
>Mature_238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIACDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=238, Percent_Identity=99.5798319327731, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1787086, Length=208, Percent_Identity=31.7307692307692, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1788226, Length=213, Percent_Identity=34.2723004694836, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788646, Length=225, Percent_Identity=32, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787030, Length=206, Percent_Identity=30.5825242718447, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=26.6968325791855, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1786874, Length=217, Percent_Identity=27.6497695852535, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082239, Length=212, Percent_Identity=27.8301886792453, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786873, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994930, Length=205, Percent_Identity=27.8048780487805, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26817; Mature: 26817

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIW
CHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHH
TVPDLLKIACDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIW
CHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHH
TVPDLLKIACDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]