Click here to switch to the map view.

The map label for this gene is pta1

Identifier: 157161785

GI number: 157161785

Start: 2456318

End: 2458462

Strand: Direct

Name: pta1

Synonym: EcHS_A2447

Alternate gene names: 157161785

Gene position: 2456318-2458462 (Clockwise)

Preceding gene: 157161784

Following gene: 157161786

Centisome position: 52.9

GC content: 55.52

Gene sequence:

>2145_bases
GTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAAT
GGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTA
CGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCC
AGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGT
TGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAA
TCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACAGCTTCGGC
GGTGCCAAAAACACCAACATCACCGGCGTTATCGTTAACAAACTGAACGCACCGGTTGATGAACAGGGTCGTACTCGCCC
GGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAAGTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCC
CGCTGCCGGTTCTCGGCGCTGTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAAT
GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGCGCACGCAGCATTCCGCACAT
GCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCCGCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAG
CCATGAACGGCGTAGAAATCGGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAA
CGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAA
CCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGA
TCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAACTTGCG
CGCAAAGCGGGCAAACGTATCGTACTGCCGGAAGGTGACGAACCGCGTACCGTTAAAGCAGCCGCTATCTGTGCTGAACG
TGGTATCGCAACTTGCGTACTGCTGGGTAATCCGGCAGAGATCAACCGTGTTGCAGCGTCTCAGGGTGTAGAACTGGGTG
CAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGC
ATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGA
TGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCA
GCTCCCTGGTATCTTCCGTGTTCTTCATGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGAT
CCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGC
TATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGCGACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGG
AAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCG
CCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGT
ACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCG
CACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAA

Upstream 100 bases:

>100_bases
AAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCG
GTAACGAAAGAGGATAAACC

Downstream 100 bases:

>100_bases
TCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAAT
CTGCATTAATCCTTTCTGAT

Product: phosphate acetyltransferase

Products: NA

Alternate protein names: Phosphotransacetylase

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLS
SNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLN
ATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCE
RAFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELA
RKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKG
MTETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPD
PTAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKA
PNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ

Sequences:

>Translated_714_residues
MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLS
SNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLN
ATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCE
RAFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELA
RKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKG
MTETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPD
PTAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKA
PNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ
>Mature_713_residues
SRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSS
NQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFGG
AKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLNA
TIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCER
AFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELAR
KAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKGM
TETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDP
TAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKAP
NSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ

Specific function: Conversion of acetate to acetyl-CoA; second step. [C]

COG id: COG0857

COG function: function code R; BioD-like N-terminal domain of phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family

Homologues:

Organism=Escherichia coli, GI1788635, Length=714, Percent_Identity=100, Blast_Score=1461, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=318, Percent_Identity=38.3647798742138, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788806, Length=349, Percent_Identity=31.2320916905444, Blast_Score=166, Evalue=6e-42,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PTA_ECOLI (P0A9M8)

Other databases:

- EMBL:   D17576
- EMBL:   D21123
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G65001
- PIR:   JX0357
- PIR:   S50130
- RefSeq:   AP_002897.1
- RefSeq:   NP_416800.1
- ProteinModelPortal:   P0A9M8
- DIP:   DIP-35815N
- IntAct:   P0A9M8
- MINT:   MINT-1263208
- STRING:   P0A9M8
- PhosSite:   P0A9M8
- SWISS-2DPAGE:   P0A9M8
- PRIDE:   P0A9M8
- EnsemblBacteria:   EBESCT00000004864
- EnsemblBacteria:   EBESCT00000018341
- GeneID:   946778
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2294
- KEGG:   eco:b2297
- EchoBASE:   EB4147
- EcoGene:   EG20173
- eggNOG:   COG0857
- GeneTree:   EBGT00050000008963
- HOGENOM:   HBG576804
- OMA:   KPIAQPH
- ProtClustDB:   PRK05632
- BioCyc:   EcoCyc:PHOSACETYLTRANS-MONOMER
- BioCyc:   MetaCyc:PHOSACETYLTRANS-MONOMER
- Genevestigator:   P0A9M8
- GO:   GO:0005737
- InterPro:   IPR010766
- InterPro:   IPR016475
- InterPro:   IPR004614
- InterPro:   IPR002505
- PIRSF:   PIRSF006107
- TIGRFAMs:   TIGR00651

Pfam domain/function: PF07085 DRTGG; PF01515 PTA_PTB

EC number: =2.3.1.8

Molecular weight: Translated: 77173; Mature: 77042

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRAN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEC
SSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
SLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFGGAKNTNITGVIVNKLNAPVD
HCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCC
EQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLN
CCCCCCCCHHHHHCCCCCCEECCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCC
ATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEI
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEE
GALLLTGGYEMDARISKLCERAFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEK
EEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEEEECCCCHHHHHHH
VQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELARKAGKRIVLPEGDEPRTVKA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCHHH
AAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKG
HHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
MTETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSV
CHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCHHHHHHHHCCCHHHHHHCCCCHHHHHHH
FFMLLPEQVYVYGDCAINPDPTAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGS
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCH
DVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTG
HHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
NTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ
CHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPRTGGDAPDQTTTIVRAN
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEC
SSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDAEVVLVEGLVPTRKHQFAQ
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
SLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFGGAKNTNITGVIVNKLNAPVD
HCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCC
EQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVLGAVPWSFDLIATRAIDMARHLN
CCCCCCCCHHHHHCCCCCCEECCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCC
ATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSLLVTSADRPDVLVAACLAAMNGVEI
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCEE
GALLLTGGYEMDARISKLCERAFATGLPVFMVNTNTWQTSLSLQSFNLEVPVDDHERIEK
EEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEEEECCCCHHHHHHH
VQEYVANYINADWIESLTATSERSRRLSPPAFRYQLTELARKAGKRIVLPEGDEPRTVKA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCHHH
AAICAERGIATCVLLGNPAEINRVAASQGVELGAGIEIVDPEVVRESYVGRLVELRKNKG
HHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
MTETVAREQLEDNVVLGTLMLEQDEVDGLVSGAVHTTANTIRPPLQLIKTAPGSSLVSSV
CHHHHHHHHHCCCEEEEEEEECCHHHHHHHHCHHHHHHHHCCCHHHHHHCCCCHHHHHHH
FFMLLPEQVYVYGDCAINPDPTAEQLAEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGS
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCH
DVEKVREATRLAQEKRPDLMIDGPLQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTG
HHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
NTTYKAVQRSADLISIGPMLQGMRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ
CHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883769; 7918659; 9205837; 9278503; 2536666