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The map label for this gene is nuoI

Identifier: 157161769

GI number: 157161769

Start: 2437479

End: 2438021

Strand: Reverse

Name: nuoI

Synonym: EcHS_A2430

Alternate gene names: 157161769

Gene position: 2438021-2437479 (Counterclockwise)

Preceding gene: 157161770

Following gene: 157161768

Centisome position: 52.5

GC content: 53.78

Gene sequence:

>543_bases
ATGACCTTAAAAGAATTGTTAGTAGGTTTCGGCACCCAGGTTCGTAGTATCTGGATGATCGGCCTGCACGCGTTCGCAAA
ACGCGAAACGCGAATGTACCCGGAAGAGCCGGTCTATCTGCCGCCCCGTTATCGTGGTCGTATCGTTCTGACCCGCGACC
CGGACGGCGAAGAGCGTTGCGTAGCCTGTAACCTCTGTGCGGTAGCCTGCCCGGTCGGCTGTATCTCGCTGCAAAAAGCA
GAAACCAAAGACGGTCGCTGGTATCCGGAATTTTTCCGTATCAACTTCTCGCGCTGCATTTTCTGTGGTCTGTGCGAAGA
AGCCTGTCCGACCACGGCGATTCAGTTAACCCCGGATTTCGAAATGGGGGAATACAAGCGCCAGGATCTGGTTTACGAGA
AAGAGGATCTGCTGATCTCCGGTCCGGGCAAATACCCGGAATATAACTTCTACCGGATGGCAGGTATGGCAATCGACGGC
AAAGATAAGGGCGAAGCAGAGAACGAAGCCAAGCCTATCGACGTCAAGAGCCTGTTACCGTAA

Upstream 100 bases:

>100_bases
TGTCCTTCGGCTGGAAAATCTGCCTGCCGCTGACGCTGATCAACTTGCTGGTAACGGCGGCTGTCATTCTCTGGCAGGCG
CAATAAGGGGCAATAAGACC

Downstream 100 bases:

>100_bases
GGAGAGGGGCAATGGAGTTCGCTTTTTATATCTGTGGCCTGATAGCCATACTTGCGACCTTGCGAGTGATCACCCATACC
AATCCGGTACACGCACTGCT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I; NUO9

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
ETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KDKGEAENEAKPIDVKSLLP

Sequences:

>Translated_180_residues
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKA
ETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
KDKGEAENEAKPIDVKSLLP
>Mature_179_residues
TLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAE
TKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGK
DKGEAENEAKPIDVKSLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=127, Percent_Identity=40.1574803149606, Blast_Score=101, Evalue=3e-22,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=100, Blast_Score=372, Evalue=1e-105,
Organism=Escherichia coli, GI1789075, Length=146, Percent_Identity=28.0821917808219, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555194, Length=145, Percent_Identity=40.6896551724138, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864306, Length=132, Percent_Identity=40.9090909090909, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ECO57 (P0AFD8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91024
- PIR:   F85868
- RefSeq:   NP_288855.1
- RefSeq:   NP_311192.1
- ProteinModelPortal:   P0AFD8
- SMR:   P0AFD8
- EnsemblBacteria:   EBESCT00000028814
- EnsemblBacteria:   EBESCT00000059682
- GeneID:   916873
- GeneID:   957341
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3540
- KEGG:   ecs:ECs3165
- GeneTree:   EBGT00050000009896
- HOGENOM:   HBG724359
- OMA:   DFFRINF
- ProtClustDB:   PRK05888
- BioCyc:   ECOL83334:ECS3165-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20538; Mature: 20407

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERC
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCEE
VACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEHHHHHHCCCCCEEECCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
CCCCHHHHCCEEECCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRYRGRIVLTRDPDGEERC
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCCEE
VACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEHHHHHHCCCCCEEECCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDGKDKGEAENEAKPIDVKSLLP
CCCCHHHHCCEEECCCEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796