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The map label for this gene is nuoL [H]

Identifier: 157161766

GI number: 157161766

Start: 2434776

End: 2436617

Strand: Reverse

Name: nuoL [H]

Synonym: EcHS_A2427

Alternate gene names: 157161766

Gene position: 2436617-2434776 (Counterclockwise)

Preceding gene: 157161767

Following gene: 157161765

Centisome position: 52.47

GC content: 57.06

Gene sequence:

>1842_bases
ATGAACATGCTTGCCTTAACCATTATTTTGCCATTGATTGGCTTCGTCCTGCTGGCGTTCTCCCGTGGGCGCTGGTCTGA
AAACGTTTCGGCGATCGTCGGCGTTGGCTCTGTGGGCCTGGCGGCGCTGGTAACCGCCTTTATCGGCGTTGATTTCTTCG
CTAACGGCGAGCAGGCATACAGCCAGCCGCTGTGGACGTGGATGTCGGTAGGCGACTTTAACATCGGTTTTAACCTGGTG
CTGGACGGCCTGTCGCTGACCATGCTCTCGGTGGTCACTGGTGTGGGTTTCCTTATTCACATGTACGCCTCCTGGTACAT
GCGCGGTGAAGAGGGCTACTCTCGCTTCTTCGCCTACACCAACCTGTTCATCGCCAGCATGGTAGTTCTGGTTCTCGCCG
ATAACCTGCTGCTGATGTACCTCGGCTGGGAAGGCGTGGGCCTGTGCTCCTATCTGCTGATCGGTTTCTATTACACCGAT
CCGAAGAATGGCGCAGCGGCAATGAAAGCGTTCGTCGTGACCCGTGTGGGTGACGTTTTCCTCGCTTTCGCACTATTCAT
TCTTTACAACGAACTGGGCACCCTGAACTTCCGCGAAATGGTGGAACTGGCACCAGCGCACTTTGCTGACGGCAATAACA
TGCTGATGTGGGCGACGCTGATGCTGCTGGGCGGTGCGGTCGGTAAATCTGCGCAGTTGCCGTTGCAGACATGGCTTGCC
GACGCGATGGCGGGCCCGACGCCTGTCTCCGCGCTGATCCACGCCGCAACCATGGTAACCGCGGGTGTCTACCTGATCGC
CCGTACCCACGGCCTGTTCCTGATGACGCCGGAAGTTCTGCATCTGGTGGGTATTGTCGGGGCGGTTACGCTGCTGCTGG
CCGGTTTTGCCGCGCTGGTACAGACCGACATCAAACGTGTTCTCGCTTACTCTACCATGAGCCAGATTGGCTACATGTTC
CTCGCGCTTGGCGTGCAGGCATGGGATGCGGCGATTTTCCACTTGATGACCCACGCGTTCTTTAAAGCGCTGCTGTTCCT
GGCATCCGGTTCCGTCATTCTGGCCTGCCATCACGAACAGAACATCTTCAAGATGGGCGGTCTGCGTAAATCTATTCCGC
TGGTTTATCTCTGCTTCCTGGTGGGCGGCGCAGCACTGTCGGCACTACCGCTGGTCACTGCGGGCTTCTTCAGTAAGGAT
GAGATCCTCGCGGGTGCGATGGCGAATGGTCATATCAATCTGATGGTGGCAGGTCTGGTCGGTGCGTTTATGACCTCGCT
CTACACCTTCCGTATGATTTTCATCGTCTTCCACGGAAAAGAACAAATTCACGCTCACGCCGTGAAAGGGGTAACTCACA
GCCTGCCGCTGATTGTGCTGCTGATCCTTTCCACCTTCGTTGGCGCACTGATTGTACCGCCGCTGCAGGGCGTGCTTCCG
CAAACGACGGAACTGGCGCACGGCAGCATGTTGACCCTGGAAATTACCTCTGGCGTGGTCGCGGTGGTCGGCATTCTGCT
GGCAGCCTGGCTGTGGCTGGGTAAACGTACTCTGGTGACCTCCATCGCCAACAGTGCGCCGGGCCGTCTGCTGGGCACCT
GGTGGTACAACGCCTGGGGATTTGACTGGCTGTATGACAAAGTGTTCGTCAAGCCGTTCCTGGGTATTGCCTGGTTGCTG
AAACGCGATCCGCTGAACTCAATGATGAACATCCCGGCTGTCCTTTCCCGCTTTGCAGGTAAAGGTCTGCTGTTAAGTGA
GAACGGCTATCTGCGCTGGTATGTGGCATCCATGAGCATCGGTGCGGTCGTGGTGCTGGCACTGTTGATGGTACTGCGTT
GA

Upstream 100 bases:

>100_bases
GCCTCGCGGCGGCAGAAGCGAGTATCGGCCTTGCGCTGCTGCTGCAACTTCACCGTCGTCGCCAGAACCTGAACATCGAT
TCAGTAAGTGAGATGCGCGG

Downstream 100 bases:

>100_bases
GTTAAGGATTGTGGGATTGCCCCTGGGCATATAAGAACGTAGGTCGGATAAGCCGCCTTCGCGGTGCATCCGACACTACC
CCCAGTCCGAAGAGAATTTT

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLV
LDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLA
DAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMF
LALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKD
EILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLL
KRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR

Sequences:

>Translated_613_residues
MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLV
LDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLA
DAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMF
LALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKD
EILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLL
KRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR
>Mature_613_residues
MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLV
LDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLA
DAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMF
LALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKD
EILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLL
KRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=482, Percent_Identity=34.8547717842324, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1788614, Length=613, Percent_Identity=99.836867862969, Blast_Score=1216, Evalue=0.0,
Organism=Escherichia coli, GI1788829, Length=421, Percent_Identity=33.9667458432304, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788827, Length=466, Percent_Identity=28.9699570815451, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=375, Percent_Identity=28.5333333333333, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788613, Length=407, Percent_Identity=26.7813267813268, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI2367154, Length=287, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=393, Percent_Identity=26.2086513994911, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66409; Mature: 66409

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQAY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHCCCHHHH
SQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYT
HCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTDPKNGAAAMKAFVVTRVGDVF
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHH
LAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLA
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
DAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALV
HHHCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
QTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
NIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKDEILAGAMANGHINLMVAGLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
GAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWG
CCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEHHHCCCC
FDWLYDKVFVKPFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEHHHH
GAVVVLALLMVLR
HHHHHHHHHHHHC
>Mature Secondary Structure
MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALVTAFIGVDFFANGEQAY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCHHHHHHHHHHHHHHHHHCCCHHHH
SQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMYASWYMRGEEGYSRFFAYT
HCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
NLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTDPKNGAAAMKAFVVTRVGDVF
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHH
LAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLA
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
DAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALV
HHHCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
QTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
NIFKMGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKDEILAGAMANGHINLMVAGLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
GAFMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNAWG
CCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEHHHCCCC
FDWLYDKVFVKPFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEHHHH
GAVVVLALLMVLR
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]