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The map label for this gene is menB

Identifier: 157161749

GI number: 157161749

Start: 2416749

End: 2417606

Strand: Reverse

Name: menB

Synonym: EcHS_A2408

Alternate gene names: 157161749

Gene position: 2417606-2416749 (Counterclockwise)

Preceding gene: 157161750

Following gene: 157161748

Centisome position: 52.06

GC content: 54.9

Gene sequence:

>858_bases
ATGATTTATCCTGATGAAGCAATGCTTTACGCACCGGTTGAATGGCACGACTGCTCCGAAGGTTTCGAGGACATTCGTTA
TGAAAAATCCACCGACGGTATCGCAAAAATCACCATTAATCGTCCGCAGGTGCGCAATGCCTTCCGTCCTCTGACGGTAA
AAGAGATGATCCAGGCACTGGCAGATGCGCGTTATGACGACAATATCGGCGTGATCATTCTGACTGGCGCAGGCGATAAA
GCGTTCTGCTCCGGTGGTGACCAGAAAGTGCGTGGTGATTACGGCGGCTATAAAGATGATTCCGGCGTACATCACTTGAA
CGTGCTGGACTTCCAGCGTCAGATCCGTACCTGTCCGAAACCGGTTGTCGCGATGGTGGCTGGCTACTCCATCGGCGGTG
GTCACGTTCTGCACATGATGTGCGACCTGACTATCGCAGCAGATAATGCCATCTTCGGTCAGACTGGCCCGAAAGTCGGT
TCCTTCGACGGCGGCTGGGGCGCTTCCTACATGGCTCGTATCGTCGGGCAGAAAAAAGCGCGAGAAATCTGGTTCCTGTG
CCGTCAGTACGACGCAAAACAGGCGCTGGATATGGGCCTTGTCAACACCGTGGTACCGCTGGCGGATCTGGAAAAAGAAA
CCGTCCGTTGGTGCCGCGAAATGCTGCAAAACAGCCCGATGGCGCTGCGCTGCCTGAAAGCTGCGCTGAACGCCGACTGT
GACGGGCAGGCGGGGCTGCAGGAGCTGGCGGGCAACGCCACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCG
CAACGCTTTCAACCAGAAACGTCAGCCTGATTTCAGCAAATTTAAACGGAATCCGTAA

Upstream 100 bases:

>100_bases
ATGTCATTCCTCGCGCCGGACATAACGCGCATCGGGAAAATCCCGCTGGCGTAATCGCAAGTCTGGCGCAGATCTTGCGT
TTCTGACTAAAGGACACAAT

Downstream 100 bases:

>100_bases
TGCGTAGTGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGC
GACGGGCTGTACGTTTGCCT

Product: naphthoate synthase

Products: NA

Alternate protein names: DHNA-CoA synthase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP

Sequences:

>Translated_285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
>Mature_285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA)

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=271, Percent_Identity=32.4723247232472, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=30.952380952381, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=30.4545454545455, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=30.4545454545455, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=30.4545454545455, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=28.1853281853282, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=25, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=25, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI70995211, Length=240, Percent_Identity=27.5, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=142, Percent_Identity=28.8732394366197, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=142, Percent_Identity=28.8732394366197, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538573, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=24.6808510638298, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=24.6808510638298, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757968, Length=222, Percent_Identity=26.5765765765766, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=222, Percent_Identity=26.5765765765766, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=285, Percent_Identity=100, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=31.9838056680162, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=27.2373540856031, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142681, Length=248, Percent_Identity=29.4354838709677, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=29.5719844357977, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=229, Percent_Identity=24.8908296943231, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=30.188679245283, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=241, Percent_Identity=26.1410788381743, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=27.3062730627306, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=197, Percent_Identity=25.3807106598985, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=24.2152466367713, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=33.0827067669173, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=33.0827067669173, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653139, Length=278, Percent_Identity=26.978417266187, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=28.6259541984733, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=28.2608695652174, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=178, Percent_Identity=30.8988764044944, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENB_ECOL6 (P0ABU1)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_754691.1
- ProteinModelPortal:   P0ABU1
- SMR:   P0ABU1
- MINT:   MINT-1227404
- EnsemblBacteria:   EBESCT00000044259
- GeneID:   1038333
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2805
- GeneTree:   EBGT00050000009097
- HOGENOM:   HBG748731
- OMA:   QWCREIL
- ProtClustDB:   PRK07396
- BRENDA:   4.1.3.36
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198
- Gene3D:   G3DSA:1.10.12.10
- TIGRFAMs:   TIGR01929

Pfam domain/function: PF00378 ECH

EC number: =4.1.3.36

Molecular weight: Translated: 31633; Mature: 31633

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: BINDING 155-155 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQAL
CCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHH
ADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPK
HCCCCCCCCCEEEEECCCCCHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCH
PVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVGSFDGGWGASYMARIVGQKKA
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
REIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
CCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQAL
CCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHH
ADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPK
HCCCCCCCCCEEEEECCCCCHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCH
PVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVGSFDGGWGASYMARIVGQKKA
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
REIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
CCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157