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The map label for this gene is pmrD

Identifier: 157161746

GI number: 157161746

Start: 2414059

End: 2414325

Strand: Reverse

Name: pmrD

Synonym: EcHS_A2405

Alternate gene names: 157161746

Gene position: 2414325-2414059 (Counterclockwise)

Preceding gene: 157161747

Following gene: 157161738

Centisome position: 51.99

GC content: 46.82

Gene sequence:

>267_bases
ATGGAATGGCTGGTCAAAAAATCGTGCTGCAATAAACAAGATAACAGACATGTACTTATGCTCTGCGATGCTGGCGGCGC
GATAAAAATGATCGCCGAAGTGAAGAGTGACTTCGCCGTGAAAGTGGGGGATTTACTCTCGCCTTTGCAGAATGCGCTTT
ATTGTATTAATCGTGAAAAGCTGCATACGGTGAAAGTCCTCTCGGCAAGCAGTTATAGCCCCGATGAGTGGGAACGGCAG
TGTAAAGTGGCAGGGAAAACTCAGTAA

Upstream 100 bases:

>100_bases
CGTTTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATGTGAAAACTC
TTAGCAAACAGGATAATGCA

Downstream 100 bases:

>100_bases
CGTTGTTTTGTCATGGGCAGGAAAATCAGCATCAACCCGCTCATAATACAGGCTACTCCCAGTAGTGCTTTCAGCGAAAA
AGTCCCTTCCCAGCCGGGAA

Product: polymyxin B resistance protein pmrD

Products: NA

Alternate protein names: BasR post-transcriptional activator; Polymyxin resistance protein pmrD

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CKVAGKTQ

Sequences:

>Translated_88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CKVAGKTQ
>Mature_88_residues
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQ
CKVAGKTQ

Specific function: Interacts with phosphorylated BasR protein to mediate transcriptional induction of BasR-activated genes to induce polymyxin resistance in some natural isolates

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pmrD family

Homologues:

Organism=Escherichia coli, GI87082076, Length=88, Percent_Identity=100, Blast_Score=184, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PMRD_ECOLI (P37590)

Other databases:

- EMBL:   L35031
- EMBL:   AY725349
- EMBL:   AY725350
- EMBL:   AY725351
- EMBL:   AY725352
- EMBL:   AY725353
- EMBL:   AY725354
- EMBL:   AY725355
- EMBL:   AY725356
- EMBL:   AY725357
- EMBL:   AY725358
- EMBL:   AY725359
- EMBL:   AY725360
- EMBL:   AY725361
- EMBL:   AY725362
- EMBL:   AY725363
- EMBL:   AY725364
- EMBL:   AY725365
- EMBL:   AY725366
- EMBL:   AY725367
- EMBL:   AY725368
- EMBL:   AY725369
- EMBL:   AY725370
- EMBL:   AY725371
- EMBL:   AY725372
- EMBL:   AY725373
- EMBL:   AY725374
- EMBL:   AY725375
- EMBL:   AY725376
- EMBL:   AY725377
- EMBL:   AY725378
- EMBL:   AY725379
- EMBL:   AY725380
- EMBL:   AY725381
- EMBL:   AY725382
- EMBL:   AY725383
- EMBL:   AY725384
- EMBL:   AY725385
- EMBL:   AY725386
- EMBL:   AY725387
- EMBL:   AY725388
- EMBL:   AY725389
- EMBL:   AY725390
- EMBL:   AY725391
- EMBL:   AY725392
- EMBL:   AY725393
- EMBL:   AY725394
- EMBL:   AY725395
- EMBL:   AY725396
- EMBL:   AY725397
- EMBL:   AY725398
- EMBL:   AY725399
- EMBL:   AY725400
- EMBL:   AY725401
- EMBL:   AY725402
- EMBL:   AY725403
- EMBL:   AY725404
- EMBL:   AY725405
- EMBL:   AY725406
- EMBL:   AY725407
- EMBL:   AY725408
- EMBL:   AY725409
- EMBL:   AY725410
- EMBL:   AY725411
- EMBL:   AY725412
- EMBL:   AY725413
- EMBL:   AY725414
- EMBL:   AY725415
- EMBL:   AY725416
- EMBL:   AY725417
- EMBL:   AY725418
- EMBL:   AY725419
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64997
- RefSeq:   AP_002857.1
- RefSeq:   NP_416762.2
- PDB:   2JSO
- PDBsum:   2JSO
- ProteinModelPortal:   P37590
- SMR:   P37590
- EnsemblBacteria:   EBESCT00000004360
- EnsemblBacteria:   EBESCT00000016143
- GeneID:   945130
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2254
- KEGG:   eco:b2259
- EchoBASE:   EB2537
- EcoGene:   EG12671
- GeneTree:   EBGT00050000009346
- OMA:   NTMEWLV
- ProtClustDB:   PRK15450
- BioCyc:   EcoCyc:G7172-MONOMER
- Genevestigator:   P37590
- InterPro:   IPR020146
- ProDom:   PD028730

Pfam domain/function: PF11183 PmrD

EC number: NA

Molecular weight: Translated: 9871; Mature: 9871

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREK
CCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCC
LHTVKVLSASSYSPDEWERQCKVAGKTQ
EEEEEEEECCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGDLLSPLQNALYCINREK
CCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCC
LHTVKVLSASSYSPDEWERQCKVAGKTQ
EEEEEEEECCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503