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The map label for this gene is arnE

Identifier: 157161744

GI number: 157161744

Start: 2413344

End: 2413679

Strand: Direct

Name: arnE

Synonym: EcHS_A2403

Alternate gene names: 157161744

Gene position: 2413344-2413679 (Clockwise)

Preceding gene: 157161743

Following gene: 157161745

Centisome position: 51.97

GC content: 53.57

Gene sequence:

>336_bases
ATGATCTGGCTAACATTAGTCTTTGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGT
GGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCT
GGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCCTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCT
GCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGTGGCATTGTGATCCT
CGGGAGTACGGTGTAA

Upstream 100 bases:

>100_bases
TTTCGGTTGACCGCGATGAAGATATCAACAGTCTCGCCATTCCGCCCGCAGATGCCATCGATCGTCAGGAGCGTCTGGTG
CTGATTCAGTATCGTCCCAA

Downstream 100 bases:

>100_bases
TGGGCCTGATATGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCAAGTCATCTG
CCGCCGATGACGCACTTATG

Product: SMR family multidrug efflux pump

Products: NA

Alternate protein names: L-Ara4N-phosphoundecaprenol flippase subunit ArnE; Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLALACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLA
AVKLWHEPVSPRHWCGVAFIIGGIVILGSTV

Sequences:

>Translated_111_residues
MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLALACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLA
AVKLWHEPVSPRHWCGVAFIIGGIVILGSTV
>Mature_111_residues
MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLALACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLA
AVKLWHEPVSPRHWCGVAFIIGGIVILGSTV

Specific function: Translocates 4-amino-4-deoxy-L-arabinose- phosphoundecaprenol (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the periplasmic side of the inner membrane

COG id: COG0697

COG function: function code GER; Permeases of the drug/metabolite transporter (DMT) superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DUF6 domain

Homologues:

Organism=Escherichia coli, GI87082074, Length=111, Percent_Identity=100, Blast_Score=217, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARNE_ECO24 (A7ZP76)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463605.1
- ProteinModelPortal:   A7ZP76
- STRING:   A7ZP76
- EnsemblBacteria:   EBESCT00000022452
- GeneID:   5590443
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2553
- eggNOG:   COG0697
- GeneTree:   EBGT00050000011874
- HOGENOM:   HBG682839
- OMA:   YPMLSIN
- ProtClustDB:   PRK15051
- BioCyc:   ECOL331111:ECE24377A_2553-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01869
- InterPro:   IPR000620
- InterPro:   IPR022883

Pfam domain/function: PF00892 DUF6

EC number: NA

Molecular weight: Translated: 12192; Mature: 12192

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x160b00d8)-; HASH(0x147dcebc)-; HASH(0x161a60a4)-;

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLALACLGLAMVLWLLVLQN
CHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIGGIVILGSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLALACLGLAMVLWLLVLQN
CHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIGGIVILGSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA