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The map label for this gene is atoB [H]

Identifier: 157161708

GI number: 157161708

Start: 2367091

End: 2368275

Strand: Direct

Name: atoB [H]

Synonym: EcHS_A2365

Alternate gene names: 157161708

Gene position: 2367091-2368275 (Clockwise)

Preceding gene: 157161707

Following gene: 157161715

Centisome position: 50.98

GC content: 53.25

Gene sequence:

>1185_bases
ATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTATCGGTAGTTTTAACGGTTCACTCGCTTCCACCAGCGCCAT
CGACCTGGGGGCGACAGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGG
GTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGC
GGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGC
GCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC
GTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATT
ACCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGC
GGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAATGTTGTCACTCGGAAGAAAACCTTCGTCT
TCAGTCAAGACGAATTCCCGAAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGA
ACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATGGAAGAATCTGCGGCGCTGGC
AGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAG
TACCTGCCACGCAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT
GCTGCACAGTTCCTTGCCGTTGGGAAAACCCTGGGCTTTGATTCTGAGAAAGTGAATGTCAACGGCGGGGCCATCGCGCT
CGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTCACACTATTACATGCCATGCAGGCACGCGATAAAACGCTGG
GGCTGGCAACACTGTGCATTGGCGGCGGCCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAA

Upstream 100 bases:

>100_bases
CATGGGCTACTGCATCACTGCCCTGCTCTTCTCCGGTGTCATTTTCGTCATTGGTTTAACGCTGTTCTGACGGCACCCCT
ACAAACAGAAGGAATATAAA

Downstream 100 bases:

>100_bases
TCAATAAAAACACCCGATAGCGAAAGTTATCGGGTGTTTTCTTGAACATCGACGGCGAAGGTAACCCCATTAATCACCAG
TCAAAACTTTTCACCAGCGT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN

Sequences:

>Translated_394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
>Mature_394_residues
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVC
GFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAF
AAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=54.1025641025641, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=43.4343434343434, Blast_Score=311, Evalue=7e-85,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=44.4162436548223, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=41.1471321695761, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=32.4009324009324, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=32.3232323232323, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1788554, Length=394, Percent_Identity=99.746192893401, Blast_Score=787, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=57.9081632653061, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=45.7920792079208, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.962962962963, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=35.6973995271868, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=45.8015267175573, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=44.0203562340967, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=42.3857868020305, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=33.9108910891089, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=32.3943661971831, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=44.7236180904523, Blast_Score=302, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=38.0352644836272, Blast_Score=222, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=53.0456852791878, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640423, Length=394, Percent_Identity=46.7005076142132, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=43.6224489795918, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40340; Mature: 40340

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAG
CCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
LGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSL
CCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
APYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGITAENVAKEYGITREMQDELA
CCEEEECCCCCCCCCCCCCEEEHHHHCCEEEEECCEEECCCHHHHHHHHCCCHHHHHHHH
LHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKA
CEECCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHH
LQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILV
HHHHCCEEECEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHH
TLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure
MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKIDSQHVDEVIMGNVLQAG
CCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
LGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAIQAGQAQSIVAGGMENMSL
CCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCC
APYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGITAENVAKEYGITREMQDELA
CCEEEECCCCCCCCCCCCCEEEHHHHCCEEEEECCEEECCCHHHHHHHHCCCHHHHHHHH
LHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDEFPKANSTAEALGALRPAFDKAG
HHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
TVTAGNASGINDGAAALVIMEESAALAAGLTPLARIKSYASGGVPPALMGMGPVPATQKA
CEECCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCCCCHHHHCCCCCCHHHHH
LQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFDSEKVNVNGGAIALGHPIGASGARILV
HHHHCCEEECEEEEECCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHH
TLLHAMQARDKTLGLATLCIGGGQGIAMVIERLN
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]