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The map label for this gene is atoA

Identifier: 157161706

GI number: 157161706

Start: 2365091

End: 2365741

Strand: Direct

Name: atoA

Synonym: EcHS_A2363

Alternate gene names: 157161706

Gene position: 2365091-2365741 (Clockwise)

Preceding gene: 157161705

Following gene: 157161707

Centisome position: 50.93

GC content: 53.46

Gene sequence:

>651_bases
ATGGATGCGAAACAACGTATTGCGCGCCGTGTGGCGCAAGAGCTTCGTGATGGTGACATCGTTAACTTAGGGATCGGTTT
ACCCACAATGGTCGCCAATTATTTACCGGAGGGTATTCATATCACTCTGCAATCGGAAAACGGCTTCCTCGGTTTAGGCC
CGGTCACGACAGCGCATCCAGATCTGGTGAACGCTGGCGGGCAACCGTGCGGTGTTTTACCCGGTGCAGCCATGTTTGAT
AGCGCCATGTCATTTGCGCTAATCCGTGGCGGTCATATTGATGCCTGCGTGCTCGGCGGTTTGCAAGTAGACGAAGAAGC
AAACCTCGCGAACTGGGTAGTACCTGGGAAAATGGTGCCCGGTATGGGTGGCGCGATGGATCTGGTGACCGGGTCGCGCA
AAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCG
CAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGC
CGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGG
GTGATTTATGA

Upstream 100 bases:

>100_bases
AGCCAGATGAACTGGTCGAAACCGGCGAGCTGCAACCTGACCATATTGTCACCCCTGGTGCCGTTATCGACCACATCATC
GTTTCACAGGAGAGCAAATA

Downstream 100 bases:

>100_bases
TTGGTCGCATATCGCGTTTTATGACGCGTTTTGTCAGCCGGTGGCTTCCCGATCCACTGATCTTTGCCATGTTGCTGACA
TTGCTAACATTCGTGATCGC

Product: 3-oxoacid CoA-transferase, B subunit

Products: NA

Alternate protein names: Acetyl-CoA:acetoacetate-CoA transferase subunit beta

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQSENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFD
SAMSFALIRGGHIDACVLGGLQVDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL

Sequences:

>Translated_216_residues
MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQSENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFD
SAMSFALIRGGHIDACVLGGLQVDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL
>Mature_216_residues
MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQSENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFD
SAMSFALIRGGHIDACVLGGLQVDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family

Homologues:

Organism=Homo sapiens, GI4557817, Length=214, Percent_Identity=47.196261682243, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI11545841, Length=213, Percent_Identity=46.0093896713615, Blast_Score=190, Evalue=9e-49,
Organism=Escherichia coli, GI1788552, Length=216, Percent_Identity=100, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17531517, Length=213, Percent_Identity=45.5399061032864, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21358325, Length=211, Percent_Identity=50.7109004739336, Blast_Score=213, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24655794, Length=211, Percent_Identity=50.7109004739336, Blast_Score=213, Evalue=6e-56,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): ATOA_ECOLI (P76459)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64992
- RefSeq:   AP_002821.1
- RefSeq:   NP_416726.1
- ProteinModelPortal:   P76459
- SMR:   P76459
- IntAct:   P76459
- STRING:   P76459
- PRIDE:   P76459
- EnsemblBacteria:   EBESCT00000003172
- EnsemblBacteria:   EBESCT00000015172
- GeneID:   946719
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2216
- KEGG:   eco:b2222
- EchoBASE:   EB1621
- EcoGene:   EG11670
- eggNOG:   COG2057
- GeneTree:   EBGT00050000012113
- HOGENOM:   HBG353770
- OMA:   HYDPTIV
- ProtClustDB:   CLSK891766
- BioCyc:   EcoCyc:ATOA-MONOMER
- BioCyc:   MetaCyc:ATOA-MONOMER
- Genevestigator:   P76459
- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164
- PANTHER:   PTHR13707
- SMART:   SM00882
- TIGRFAMs:   TIGR02428

Pfam domain/function: PF01144 CoA_trans

EC number: =2.8.3.8

Molecular weight: Translated: 22960; Mature: 22960

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01274 COA_TRANSF_2

Important sites: ACT_SITE 46-46

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQSENGFLGLGPVTTAHP
CCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCC
DLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQVDEEANLANWVVPGKMVP
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCCCCEEECCCCCC
GMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTAQHAVHMLVTELAVFRFIDGK
CCCCCEEEECCCCEEEEEEHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
MWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL
EEEHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQSENGFLGLGPVTTAHP
CCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCC
DLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQVDEEANLANWVVPGKMVP
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCCCCEEECCCCCC
GMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTAQHAVHMLVTELAVFRFIDGK
CCCCCEEEECCCCEEEEEEHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
MWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL
EEEHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503