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The map label for this gene is napH

Identifier: 157161685

GI number: 157161685

Start: 2339694

End: 2340557

Strand: Reverse

Name: napH

Synonym: EcHS_A2342

Alternate gene names: 157161685

Gene position: 2340557-2339694 (Counterclockwise)

Preceding gene: 157161686

Following gene: 157161684

Centisome position: 50.4

GC content: 55.9

Gene sequence:

>864_bases
ATGGCAAATCGTAAACGTGACGCCGGGCGCGAGGCGCTGGAGAAAAAAGGCTGGTGGCGCAGTCACCGTTGGCTGGTGTT
ACGTCGCCTTTGTCAGTTCTTCGTGCTGGGGATGTTTTTGAGCGGTCCATGGTTTGGTGTGTGGATCTTGCACGGCAACT
ACAGCAGTAGCCTGTTATTCGACACCGTTCCGCTGACCGATCCGCTGATGACGCTGCAAAGTCTTGCCAGTGGTCATCTG
CCTGCCACGGTGGCGTTGACCGGTGCGGTGATTATCACCGTGCTCTACGCCCTGGCGGGAAAGCGATTATTTTGCAGCTG
GGTCTGCCCGCTGAACCCGATTACCGACTTAGCGAACTGGTTACGCAGGCGCTTTGACCTCAATCAGTCTGCGACGATCC
CTCGCCACATTCGCTACGTGCTGTTGGTGGTCATTCTGGTGGGATCAGCCTTAACTGGCACGCTCATTTGGGAATGGATC
AACCCCGTTTCTCTGATGGGGCGTAGCCTGGTAATGGGCTTCGGCAGCGGCGCGCTGCTTATTCTCGCGCTGTTTTTATT
TGATCTACTGGTCGTTGAACACGGCTGGTGCGGGCACATTTGTCCGGTTGGCGCGCTGTATGGCGTGCTGGGTAGCAAAG
GTGTGATTACCGTTGCGGCCACCGATCGGCAGAAATGTAACCGCTGTATGGATTGTTTTCATGTTTGCCCGGAACCGCAT
GTGCTACGTGCCCCGGTGCTGGATGAACAAAGCCCGGTGCAGGTCACCAGCCGCGATTGCATGACGTGCGGTCGCTGCGT
GGATGTCTGTTCTGAGGATGTATTTACAATAACTACACGATGGAGTTCGGGAGCGAAATCATGA

Upstream 100 bases:

>100_bases
GTATGCGTGCTGGAACAACCGGCAATCAAGGTGTTACCGCTGTCACTGGCGAAAGGGGAGTTAGGTCACCATTACCGCTT
CGGCTGGCTGGAGGGGAACA

Downstream 100 bases:

>100_bases
AAAGCCATGACCTGAAGAAAGCGCTGTGTCAATGGACGGCGATGCTGGCCCTGGTGGTAAGCGGCGCGGTTTGGGCGGCT
AATGGCGTCGATTTTAGCCA

Product: quinol dehydrogenase membrane component

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHL
PATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPH
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS

Sequences:

>Translated_287_residues
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHL
PATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPH
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
>Mature_286_residues
ANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLP
ATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWIN
PVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPHV
LRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS

Specific function: Involved in electron transfer

COG id: COG0348

COG function: function code C; Polyferredoxin

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1788532, Length=287, Percent_Identity=100, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1787226, Length=265, Percent_Identity=27.1698113207547, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPH_ECOLI (P33934)

Other databases:

- EMBL:   U00008
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64990
- RefSeq:   AP_002800.1
- RefSeq:   NP_416708.1
- ProteinModelPortal:   P33934
- SMR:   P33934
- DIP:   DIP-10310N
- IntAct:   P33934
- MINT:   MINT-1276100
- STRING:   P33934
- EnsemblBacteria:   EBESCT00000004766
- EnsemblBacteria:   EBESCT00000004767
- EnsemblBacteria:   EBESCT00000004768
- EnsemblBacteria:   EBESCT00000016564
- GeneID:   945984
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2192
- KEGG:   eco:b2204
- EchoBASE:   EB1992
- EcoGene:   EG12062
- eggNOG:   COG0348
- GeneTree:   EBGT00050000011445
- HOGENOM:   HBG296648
- OMA:   WEWVNPV
- ProtClustDB:   PRK09477
- BioCyc:   EcoCyc:NAPH-MONOMER
- BioCyc:   MetaCyc:NAPH-MONOMER
- Genevestigator:   P33934
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011886
- TIGRFAMs:   TIGR02163

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31874; Mature: 31743

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16229d60)-; HASH(0x16025c4c)-; HASH(0x1096fd08)-; HASH(0x13f066a8)-;

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLF
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
DTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANW
ECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
LRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWINPVSLMGRSLVMGFGSGALL
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHH
ILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCC
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
EEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCH
>Mature Secondary Structure 
ANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLF
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
DTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANW
ECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
LRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWINPVSLMGRSLVMGFGSGALL
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHH
ILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCC
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
EEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503