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The map label for this gene is bcr

Identifier: 157161664

GI number: 157161664

Start: 2320748

End: 2321938

Strand: Reverse

Name: bcr

Synonym: EcHS_A2320

Alternate gene names: 157161664

Gene position: 2321938-2320748 (Counterclockwise)

Preceding gene: 157161665

Following gene: 157161663

Centisome position: 50.0

GC content: 53.23

Gene sequence:

>1191_bases
GTGACCACCCGACAGCATTCGTCGTTTGCTATTGTTTTTATCCTTGGCCTGCTGGCCATGTTGATGCCGCTGTCGATTGA
TATGTATCTGCCCGCGCTACCGGTAATTTCAGCGCAGTTTGGCGTACCGGCGGGCAGTACGCAGATGACCCTCAGTACTT
ATATTCTGGGCTTTGCGTTGGGGCAGTTAATCTACGGGCCGATGGCAGACAGCTTCGGGCGTAAGCCGGTGGTGCTCGGC
GGTACGCTGGTGTTTGCCGCCGCCGCGGTGGCGTGTGCGTTGGCAAACACCATCGATCAGCTGATTGTGATGCGTTTCTT
CCACGGGCTGGCTGCGGCTGCGGCCAGCGTGGTCATTAACGCCCTGATGCGCGATATTTACCCGAAAGAAGAGTTCTCGC
GGATGATGTCGTTTGTCATGCTGGTGACAACCATTGCACCGCTGATGGCACCGATAGTTGGCGGCTGGGTGCTGGTGTGG
CTGAGCTGGCATTACATCTTCTGGATCCTGGCATTAGCGGCGATTCTGGCTTCGGCAATGATTTTCTTCCTGATTAAAGA
AACCTTACCACCGGAGCGTCGTCAGCCATTTCACATTCGTACCACTATTGGTAACTTTGCAGCGCTGTTCCGCCATAAAC
GTGTCCTGAGCTACATGCTTGCCAGTGGTTTCAGCTTTGCCGGGATGTTCTCATTCTTAAGCGCCGGACCGTTTGTTTAT
ATTGAAATTAACCACGTCGCGCCGGAAAACTTTGGTTATTACTTTGCGCTAAACATTGTTTTTCTGTTCGTGATGACCAT
CTTTAACAGCCGCTTCGTCCGCCGCATTGGCGCGTTAAATATGTTCCGCTCGGGGTTGTGGATACAATTTATTATGGCAG
CGTGGATGGTCATCAGTGCGCTGCTGGGGCTGGGATTTTGGTCGCTGGTGGTTGGCGTTGCGGCGTTTGTGGGCTGCGTG
TCGATGGTGTCATCCAATGCGATGGCGGTCATTCTTGATGAGTTTCCCCATATGGCGGGAACGGCATCTTCGCTGGCAGG
AACCTTCCGTTTTGGCATAGGGGCAATTGTTGGCGCATTGCTTTCTCTTGCGACCTTTAACTCTGCATGGCCGATGATTT
GGTCAATTGCATTCTGCGCAACCAGCTCCATTCTCTTCTGTCTGTACGCCAGTCGGCCGAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TACGCTGGATGCTGATTTAGCCCCCGGTGAATATCGTCCGTTAACTGAAGAAGAAATTGCCAGCGTCGTCTAACTTTTCA
ATGACTTTCAGGAGCCCGTT

Downstream 100 bases:

>100_bases
TCTATTGCACAACGAGGAAGCTAAAAGGCTTCCTTTGTTGATGCATGTCAACCACAAATCTATCATTCCCCCGATATATG
TTTATTTTATGTAAAATCAA

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR

Sequences:

>Translated_396_residues
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLG
GTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR
>Mature_395_residues
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGG
TLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWL
SWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYI
EINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVS
MVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=100, Blast_Score=787, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=337, Percent_Identity=28.7833827893175, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790146, Length=226, Percent_Identity=30.9734513274336, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082312, Length=339, Percent_Identity=26.5486725663717, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787065, Length=303, Percent_Identity=23.7623762376238, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790794, Length=284, Percent_Identity=20.0704225352113, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=29.7101449275362, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081738, Length=357, Percent_Identity=25.4901960784314, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787880, Length=209, Percent_Identity=30.1435406698565, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=25.6281407035176, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=26.6272189349112, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325414, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321577, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=182, Percent_Identity=22.5274725274725, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=205, Percent_Identity=22.9268292682927, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BCR_ECOLI (P28246)

Other databases:

- EMBL:   X63703
- EMBL:   U00008
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64987
- RefSeq:   AP_002780.1
- RefSeq:   NP_416687.1
- ProteinModelPortal:   P28246
- SMR:   P28246
- STRING:   P28246
- EnsemblBacteria:   EBESCT00000000683
- EnsemblBacteria:   EBESCT00000015675
- GeneID:   944808
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5363
- KEGG:   eco:b2182
- EchoBASE:   EB1390
- EcoGene:   EG11419
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008820
- HOGENOM:   HBG300735
- OMA:   FCATCSI
- ProtClustDB:   PRK11102
- BioCyc:   EcoCyc:BCR-MONOMER
- Genevestigator:   P28246
- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- TIGRFAMs:   TIGR00880
- TIGRFAMs:   TIGR00710

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43353; Mature: 43222

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x156f5180)-; HASH(0x16004bac)-; HASH(0x1607e9dc)-; HASH(0x14e46514)-; HASH(0x15a3c384)-; HASH(0x16036d80)-; HASH(0x160a0294)-; HASH(0x14693e3c)-; HASH(0x15e05754)-; HASH(0x15df7e1c)-; HASH(0x160a8bec)-; HASH(0x15dfb208)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure 
TTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
GQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
IEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSLATFNSAWPMIWSIAFCATSSILFCLYASRPKKR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948